Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100036305> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2100036305 endingPage "1412" @default.
- W2100036305 startingPage "1404" @default.
- W2100036305 abstract "The GnRH-expressing neurons are the ultimate regulator of reproductive function. GnRH gene expression is limited to this small population of neurons in the hypothalamus. Transfections using 3 kb of the rat or mouse 5'-regulatory region provide specific gene expression in the hypothalamic cell line GT1-7. The combination of two elements, a 300-bp enhancer and a 173-bp promoter, recapitulates specificity in GT1-7 cells. It was not known whether these elements could specifically target gene expression throughout development in the whole animal. We demonstrate that the 3-kb rat GnRH regulatory region provides a higher degree of specificity than the equivalent mouse sequence in a mouse hypothalamic cell line. Moreover, combination of the enhancer and the promoter of the rat gene targets expression to GnRH neurons in transgenic mice in a developmentally appropriate manner. Transgene expression is regulated by activin A, a known activator of GnRH gene expression. In contrast, the enhancer on a heterologous promoter produces inappropriate expression in vivo. We conclude that the enhancer and promoter regions of the rat GnRH gene are necessary for targeted expression to hypothalamic neurons and are sufficient to confer regulated, cell type-specific expression to a reporter gene in vivo." @default.
- W2100036305 created "2016-06-24" @default.
- W2100036305 creator A5008221415 @default.
- W2100036305 creator A5017821858 @default.
- W2100036305 creator A5018272606 @default.
- W2100036305 creator A5028439881 @default.
- W2100036305 creator A5050968979 @default.
- W2100036305 creator A5091248614 @default.
- W2100036305 date "2002-04-01" @default.
- W2100036305 modified "2023-09-23" @default.
- W2100036305 title "Neuron-Specific Expression in Vivo by Defined Transcription Regulatory Elements of the GnRH Gene" @default.
- W2100036305 cites W1926946830 @default.
- W2100036305 cites W1974910300 @default.
- W2100036305 cites W1997917613 @default.
- W2100036305 cites W2004605227 @default.
- W2100036305 cites W2007123195 @default.
- W2100036305 cites W2013287098 @default.
- W2100036305 cites W2024658716 @default.
- W2100036305 cites W2069582794 @default.
- W2100036305 cites W2072753013 @default.
- W2100036305 cites W2092153968 @default.
- W2100036305 cites W2103529406 @default.
- W2100036305 cites W2106882534 @default.
- W2100036305 cites W2141115613 @default.
- W2100036305 cites W4237884234 @default.
- W2100036305 cites W4245524405 @default.
- W2100036305 doi "https://doi.org/10.1210/endo.143.4.8751" @default.
- W2100036305 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11897697" @default.
- W2100036305 hasPublicationYear "2002" @default.
- W2100036305 type Work @default.
- W2100036305 sameAs 2100036305 @default.
- W2100036305 citedByCount "30" @default.
- W2100036305 countsByYear W21000363052013 @default.
- W2100036305 countsByYear W21000363052015 @default.
- W2100036305 countsByYear W21000363052016 @default.
- W2100036305 countsByYear W21000363052018 @default.
- W2100036305 countsByYear W21000363052019 @default.
- W2100036305 countsByYear W21000363052023 @default.
- W2100036305 crossrefType "journal-article" @default.
- W2100036305 hasAuthorship W2100036305A5008221415 @default.
- W2100036305 hasAuthorship W2100036305A5017821858 @default.
- W2100036305 hasAuthorship W2100036305A5018272606 @default.
- W2100036305 hasAuthorship W2100036305A5028439881 @default.
- W2100036305 hasAuthorship W2100036305A5050968979 @default.
- W2100036305 hasAuthorship W2100036305A5091248614 @default.
- W2100036305 hasBestOaLocation W21000363051 @default.
- W2100036305 hasConcept C101762097 @default.
- W2100036305 hasConcept C102230213 @default.
- W2100036305 hasConcept C104317684 @default.
- W2100036305 hasConcept C111936080 @default.
- W2100036305 hasConcept C141035611 @default.
- W2100036305 hasConcept C150194340 @default.
- W2100036305 hasConcept C153911025 @default.
- W2100036305 hasConcept C161733203 @default.
- W2100036305 hasConcept C165864922 @default.
- W2100036305 hasConcept C21592294 @default.
- W2100036305 hasConcept C54355233 @default.
- W2100036305 hasConcept C86803240 @default.
- W2100036305 hasConcept C95444343 @default.
- W2100036305 hasConceptScore W2100036305C101762097 @default.
- W2100036305 hasConceptScore W2100036305C102230213 @default.
- W2100036305 hasConceptScore W2100036305C104317684 @default.
- W2100036305 hasConceptScore W2100036305C111936080 @default.
- W2100036305 hasConceptScore W2100036305C141035611 @default.
- W2100036305 hasConceptScore W2100036305C150194340 @default.
- W2100036305 hasConceptScore W2100036305C153911025 @default.
- W2100036305 hasConceptScore W2100036305C161733203 @default.
- W2100036305 hasConceptScore W2100036305C165864922 @default.
- W2100036305 hasConceptScore W2100036305C21592294 @default.
- W2100036305 hasConceptScore W2100036305C54355233 @default.
- W2100036305 hasConceptScore W2100036305C86803240 @default.
- W2100036305 hasConceptScore W2100036305C95444343 @default.
- W2100036305 hasIssue "4" @default.
- W2100036305 hasLocation W21000363051 @default.
- W2100036305 hasLocation W21000363052 @default.
- W2100036305 hasOpenAccess W2100036305 @default.
- W2100036305 hasPrimaryLocation W21000363051 @default.
- W2100036305 hasRelatedWork W1974703394 @default.
- W2100036305 hasRelatedWork W2036161725 @default.
- W2100036305 hasRelatedWork W2060892434 @default.
- W2100036305 hasRelatedWork W2092584888 @default.
- W2100036305 hasRelatedWork W2093633615 @default.
- W2100036305 hasRelatedWork W2140322682 @default.
- W2100036305 hasRelatedWork W2147725961 @default.
- W2100036305 hasRelatedWork W2167465117 @default.
- W2100036305 hasRelatedWork W3163786000 @default.
- W2100036305 hasRelatedWork W4244403405 @default.
- W2100036305 hasVolume "143" @default.
- W2100036305 isParatext "false" @default.
- W2100036305 isRetracted "false" @default.
- W2100036305 magId "2100036305" @default.
- W2100036305 workType "article" @default.