FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100040663> ?p ?o ?g. }

• W2100040663 endingPage "1137" @default.
• W2100040663 startingPage "1125" @default.
• W2100040663 abstract "Metallothioneins (MTs) are low-molecular-weight, cysteine-rich proteins believed to play a role in cytosolic zinc (Zn) and copper (Cu) homeostasis. However, evidence for the functional properties of MTs has been hampered by methodological problems in the isolation and characterization of the proteins. Here, we document that barley (Hordeum vulgare) MT3 and MT4 proteins exist in planta and that they differ in tissue localization as well as in metal coordination chemistry. Combined transcriptional and histological analyses showed temporal and spatial correlations between transcript levels and protein abundance during grain development. MT3 was present in tissues of both maternal and filial origin throughout grain filling. In contrast, MT4 was confined to the embryo and aleurone layer, where it appeared during tissue specialization and remained until maturity. Using state-of-the-art speciation analysis by size-exclusion chromatography inductively coupled plasma mass spectrometry and electrospray ionization time-of-flight mass spectrometry on recombinant MT3 and MT4, their specificity and capacity for metal ion binding were quantified, showing a strong preferential Zn binding relative to Cu and cadmium (Cd) in MT4, which was not the case for MT3. When complementary DNAs from barley MTs were expressed in Cu- or Cd-sensitive yeast mutants, MT3 provided a much stronger complementation than did MT4. We conclude that MT3 may play a housekeeping role in metal homeostasis, while MT4 may function in Zn storage in developing and mature grains. The localization of MT4 and its discrimination against Cd make it an ideal candidate for future biofortification strategies directed toward increasing food and feed Zn concentrations." @default.
• W2100040663 created "2016-06-24" @default.
• W2100040663 creator A5008567312 @default.
• W2100040663 creator A5010036182 @default.
• W2100040663 creator A5055116772 @default.
• W2100040663 creator A5070395842 @default.
• W2100040663 creator A5085416485 @default.
• W2100040663 creator A5088628110 @default.
• W2100040663 creator A5091650390 @default.
• W2100040663 date "2012-05-11" @default.
• W2100040663 modified "2023-10-12" @default.
• W2100040663 title "Barley Metallothioneins: MT3 and MT4 Are Localized in the Grain Aleurone Layer and Show Differential Zinc Binding " @default.
• W2100040663 cites W1490297081 @default.
• W2100040663 cites W1880282918 @default.
• W2100040663 cites W1965073499 @default.
• W2100040663 cites W1971691741 @default.
• W2100040663 cites W1971884395 @default.
• W2100040663 cites W1994429618 @default.
• W2100040663 cites W1996223187 @default.
• W2100040663 cites W2000456081 @default.
• W2100040663 cites W2003009756 @default.
• W2100040663 cites W2013055299 @default.
• W2100040663 cites W2013164482 @default.
• W2100040663 cites W2013948741 @default.
• W2100040663 cites W2017748038 @default.
• W2100040663 cites W2019591778 @default.
• W2100040663 cites W2025004420 @default.
• W2100040663 cites W2027577142 @default.
• W2100040663 cites W2038591876 @default.
• W2100040663 cites W2042388618 @default.
• W2100040663 cites W2045009949 @default.
• W2100040663 cites W2049741584 @default.
• W2100040663 cites W2051126279 @default.
• W2100040663 cites W2052865318 @default.
• W2100040663 cites W2053686601 @default.
• W2100040663 cites W2058401582 @default.
• W2100040663 cites W2065743337 @default.
• W2100040663 cites W2079387486 @default.
• W2100040663 cites W2083167998 @default.
• W2100040663 cites W2083278094 @default.
• W2100040663 cites W2085771792 @default.
• W2100040663 cites W2088209162 @default.
• W2100040663 cites W2092729576 @default.
• W2100040663 cites W2098575506 @default.
• W2100040663 cites W2099593668 @default.
• W2100040663 cites W2099888478 @default.
• W2100040663 cites W2100205328 @default.
• W2100040663 cites W2101407526 @default.
• W2100040663 cites W2105052969 @default.
• W2100040663 cites W2105338725 @default.
• W2100040663 cites W2108244474 @default.
• W2100040663 cites W2113186717 @default.
• W2100040663 cites W2114055738 @default.
• W2100040663 cites W2121571447 @default.
• W2100040663 cites W2123662095 @default.
• W2100040663 cites W2124946184 @default.
• W2100040663 cites W2125121305 @default.
• W2100040663 cites W2127239419 @default.
• W2100040663 cites W2131429570 @default.
• W2100040663 cites W2136056539 @default.
• W2100040663 cites W2136727751 @default.
• W2100040663 cites W2144362290 @default.
• W2100040663 cites W2146503498 @default.
• W2100040663 cites W2147702594 @default.
• W2100040663 cites W2148710028 @default.
• W2100040663 cites W2150163895 @default.
• W2100040663 cites W2154086735 @default.
• W2100040663 cites W2155181409 @default.
• W2100040663 cites W2157113999 @default.
• W2100040663 cites W2161645265 @default.
• W2100040663 cites W2163208441 @default.
• W2100040663 cites W2164395223 @default.
• W2100040663 cites W2165172658 @default.
• W2100040663 cites W2169887349 @default.
• W2100040663 cites W4242962521 @default.
• W2100040663 doi "https://doi.org/10.1104/pp.112.197798" @default.
• W2100040663 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3387699" @default.
• W2100040663 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22582132" @default.
• W2100040663 hasPublicationYear "2012" @default.
• W2100040663 type Work @default.
• W2100040663 sameAs 2100040663 @default.
• W2100040663 citedByCount "48" @default.
• W2100040663 countsByYear W21000406632013 @default.
• W2100040663 countsByYear W21000406632014 @default.
• W2100040663 countsByYear W21000406632015 @default.
• W2100040663 countsByYear W21000406632016 @default.
• W2100040663 countsByYear W21000406632017 @default.
• W2100040663 countsByYear W21000406632018 @default.
• W2100040663 countsByYear W21000406632019 @default.
• W2100040663 countsByYear W21000406632020 @default.
• W2100040663 countsByYear W21000406632021 @default.
• W2100040663 countsByYear W21000406632022 @default.
• W2100040663 countsByYear W21000406632023 @default.
• W2100040663 crossrefType "journal-article" @default.
• W2100040663 hasAuthorship W2100040663A5008567312 @default.
• W2100040663 hasAuthorship W2100040663A5010036182 @default.
• W2100040663 hasAuthorship W2100040663A5055116772 @default.
• W2100040663 hasAuthorship W2100040663A5070395842 @default.

• next