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- W2100043746 abstract "The gene is a highly polymorphic major histocompatibility complex (MHC) class II gene and plays an important role in variability of immune responsiveness and disease resistance. In the present study, the MHC class II gene in water buffalo populations from Northwest regions of Iran was investigated through PCR-SSCP. Genomic DNA was extracted from whole blood samples collected from 50 buffaloes. A 284 bp segment of exon 2 of was amplified by standard PCR, using locus-specific primers. The PCR products were subjected to a non-denaturing gel electrophoresis. A number of 11 different SSCP patterns indicating allelic variation were identified. The three most frequent patterns 1, 4 and 10 accounted for 58% of the total patterns. Results indicated that exon 2 of the gene is highly polymorphic among the examined animals. DRB3" @default.
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- W2100043746 date "2010-01-01" @default.
- W2100043746 modified "2023-09-24" @default.
- W2100043746 title "ANALYSIS OF POLYMORPHISM OF MHC CLASS II BULA DRB3 EXON 2 GENE IN NORTH WEST IRANIAN POPULATIONS OF THE WATER BUFFALO THROUGH PCR-SSCP (BUBALUS BUBALIS)" @default.
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