FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100050857> ?p ?o ?g. }

• W2100050857 endingPage "335" @default.
• W2100050857 startingPage "325" @default.
• W2100050857 abstract "Anaplasma marginale is the causative agent of bovine anaplasmosis, a disease which can be protected by vaccination with the less pathogenic Anaplasma species, A. centrale. Currently, there is no polymerase chain reaction (PCR) assay available which differentiates between different species of Anaplasma or which can differentiate isolates of A. marginale within outbreaks and between different countries. A molecular test specific for A. marginale would be ideal for the identification of Anaplasma species in wild ruminants, as possible reservoirs of anaplasmosis, and to differentiate between A. marginale from A. centrale. A PCR assay was designed to amplify the major surface protein 1alpha gene of the rickettsial bovine pathogen, A. marginale both as an inter- and intra-specific test. The test did not amplify A. centrale or A. ovis, and discriminated A. marginale by amplifying repeat regions within the msp1alpha gene which vary in number between many isolates. The nested A. marginale amplicons varied in size from 630 to 1190bp representing one to eight internal repeats. All 22 Australian isolates tested amplified a 630bp product (one repeat) in contrast to all 19 non-Australian isolates tested. Eight sequences from Australian isolates from different geographical regions confirmed the conserved nature of the Australian A. marginale msp1alpha genes. The Australian 'repeat unit' MSP1a deduced amino acid sequence has been designated as Australian type 1. The msp1alpha PCR method developed here enabled the amplification and comparison of A. marginale isolates originating from North and South America, Africa, Israel and Australia. The method is sensitive and specific for A. marginale. Although additional msp1alpha products were amplified from at least two Australian isolates, the results suggest limited introduction of A. marginale into Australia." @default.
• W2100050857 created "2016-06-24" @default.
• W2100050857 creator A5007223482 @default.
• W2100050857 creator A5008769046 @default.
• W2100050857 creator A5049150531 @default.
• W2100050857 creator A5032010438 @default.
• W2100050857 date "2002-05-01" @default.
• W2100050857 modified "2023-10-18" @default.
• W2100050857 title "A msp1α polymerase chain reaction assay for specific detection and differentiation of Anaplasma marginale isolates" @default.
• W2100050857 cites W145515610 @default.
• W2100050857 cites W1805763996 @default.
• W2100050857 cites W1858013354 @default.
• W2100050857 cites W1907706797 @default.
• W2100050857 cites W1953990936 @default.
• W2100050857 cites W1964235168 @default.
• W2100050857 cites W1967808868 @default.
• W2100050857 cites W1981024135 @default.
• W2100050857 cites W1984681199 @default.
• W2100050857 cites W2010426365 @default.
• W2100050857 cites W2018147322 @default.
• W2100050857 cites W2023591054 @default.
• W2100050857 cites W2027015918 @default.
• W2100050857 cites W2036500090 @default.
• W2100050857 cites W2052276531 @default.
• W2100050857 cites W2055043387 @default.
• W2100050857 cites W2059858984 @default.
• W2100050857 cites W2069536855 @default.
• W2100050857 cites W2074570887 @default.
• W2100050857 cites W2112651984 @default.
• W2100050857 cites W2132554048 @default.
• W2100050857 cites W2133427302 @default.
• W2100050857 cites W2143035914 @default.
• W2100050857 cites W2155686097 @default.
• W2100050857 cites W2158714788 @default.
• W2100050857 cites W2164529987 @default.
• W2100050857 cites W2170417972 @default.
• W2100050857 cites W2418189535 @default.
• W2100050857 cites W4248441758 @default.
• W2100050857 doi "https://doi.org/10.1016/s0378-1135(02)00017-2" @default.
• W2100050857 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11955782" @default.
• W2100050857 hasPublicationYear "2002" @default.
• W2100050857 type Work @default.
• W2100050857 sameAs 2100050857 @default.
• W2100050857 citedByCount "96" @default.
• W2100050857 countsByYear W21000508572012 @default.
• W2100050857 countsByYear W21000508572013 @default.
• W2100050857 countsByYear W21000508572014 @default.
• W2100050857 countsByYear W21000508572015 @default.
• W2100050857 countsByYear W21000508572016 @default.
• W2100050857 countsByYear W21000508572017 @default.
• W2100050857 countsByYear W21000508572018 @default.
• W2100050857 countsByYear W21000508572019 @default.
• W2100050857 countsByYear W21000508572020 @default.
• W2100050857 countsByYear W21000508572021 @default.
• W2100050857 countsByYear W21000508572022 @default.
• W2100050857 countsByYear W21000508572023 @default.
• W2100050857 crossrefType "journal-article" @default.
• W2100050857 hasAuthorship W2100050857A5007223482 @default.
• W2100050857 hasAuthorship W2100050857A5008769046 @default.
• W2100050857 hasAuthorship W2100050857A5032010438 @default.
• W2100050857 hasAuthorship W2100050857A5049150531 @default.
• W2100050857 hasConcept C104317684 @default.
• W2100050857 hasConcept C159047783 @default.
• W2100050857 hasConcept C2776732713 @default.
• W2100050857 hasConcept C2777479583 @default.
• W2100050857 hasConcept C2777511614 @default.
• W2100050857 hasConcept C2779620486 @default.
• W2100050857 hasConcept C2781329793 @default.
• W2100050857 hasConcept C49105822 @default.
• W2100050857 hasConcept C54355233 @default.
• W2100050857 hasConcept C8185291 @default.
• W2100050857 hasConcept C86803240 @default.
• W2100050857 hasConceptScore W2100050857C104317684 @default.
• W2100050857 hasConceptScore W2100050857C159047783 @default.
• W2100050857 hasConceptScore W2100050857C2776732713 @default.
• W2100050857 hasConceptScore W2100050857C2777479583 @default.
• W2100050857 hasConceptScore W2100050857C2777511614 @default.
• W2100050857 hasConceptScore W2100050857C2779620486 @default.
• W2100050857 hasConceptScore W2100050857C2781329793 @default.
• W2100050857 hasConceptScore W2100050857C49105822 @default.
• W2100050857 hasConceptScore W2100050857C54355233 @default.
• W2100050857 hasConceptScore W2100050857C8185291 @default.
• W2100050857 hasConceptScore W2100050857C86803240 @default.
• W2100050857 hasIssue "4" @default.
• W2100050857 hasLocation W21000508571 @default.
• W2100050857 hasLocation W21000508572 @default.
• W2100050857 hasOpenAccess W2100050857 @default.
• W2100050857 hasPrimaryLocation W21000508571 @default.
• W2100050857 hasRelatedWork W1830682822 @default.
• W2100050857 hasRelatedWork W1963174328 @default.
• W2100050857 hasRelatedWork W1990690755 @default.
• W2100050857 hasRelatedWork W1996362265 @default.
• W2100050857 hasRelatedWork W2085731833 @default.
• W2100050857 hasRelatedWork W2100050857 @default.
• W2100050857 hasRelatedWork W2148844706 @default.
• W2100050857 hasRelatedWork W2264980412 @default.
• W2100050857 hasRelatedWork W2323557000 @default.
• W2100050857 hasRelatedWork W4378516877 @default.

• next