FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100057614> ?p ?o ?g. }

• W2100057614 endingPage "259" @default.
• W2100057614 startingPage "247" @default.
• W2100057614 abstract "A putative 8,7SI (sterol 8,7-isomerase) from Zea mays, termed Zm8,7SI, has been isolated from an EST (expressed sequence tag) library and subcloned into the yeast erg2 mutant lacking 8,7SI activity. Zm8,7SI restored endogenous ergosterol synthesis. An in vitro enzymatic assay in the corresponding yeast microsomal extract indicated that the preferred Δ8-sterol substrate possesses a single C4α methyl group, in contrast with 8,7SIs from animals and fungi, thus reflecting the diversity in the structure of their active site in relation to the distinct sterol biosynthetic pathways. In accordance with the proposed catalytic mechanism, a series of lipophilic ammonium-ion-containing derivatives possessing a variety of structures and biological properties, potently inhibited the Zm8,7SI in vitro. To evaluate the importance of a series of conserved acidic and tryptophan residues which could be involved in the Zm8,7SI catalytic mechanism, 20 mutants of Zm8,7SI were constructed as well as a number of corresponding mutants of the Saccharomyces cerevisiae 8,7SI. The mutated isomerases were assayed in vivo by sterol analysis and quantification of Δ5,7-sterols and directly in vitro by examination of the activities of the recombinant Zm8,7SI mutants. These studies have identified His74, Glu78, Asp107, Glu121, Trp66 and Trp193 that are required for Zm8,7SI activity and show that binding of the enzyme–substrate complex is impaired in the mutant T124I. They underline the functional homology between the plant and animal 8,7SIs on one hand, in contrast with the yeast 8,7SI on the other hand, in accordance with their molecular diversity and distinct mechanisms." @default.
• W2100057614 created "2016-06-24" @default.
• W2100057614 creator A5041630420 @default.
• W2100057614 creator A5057386212 @default.
• W2100057614 creator A5080724123 @default.
• W2100057614 creator A5090863272 @default.
• W2100057614 date "2008-08-12" @default.
• W2100057614 modified "2023-09-25" @default.
• W2100057614 title "Identification of essential amino acid residues in a sterol 8,7-isomerase from <i>Zea mays</i> reveals functional homology and diversity with the isomerases of animal and fungal origin" @default.
• W2100057614 cites W110934164 @default.
• W2100057614 cites W1499672184 @default.
• W2100057614 cites W1577282977 @default.
• W2100057614 cites W1644961050 @default.
• W2100057614 cites W1871616926 @default.
• W2100057614 cites W1964353889 @default.
• W2100057614 cites W1966937225 @default.
• W2100057614 cites W1976934653 @default.
• W2100057614 cites W1989294901 @default.
• W2100057614 cites W1989507808 @default.
• W2100057614 cites W1990017308 @default.
• W2100057614 cites W2011187123 @default.
• W2100057614 cites W2017139247 @default.
• W2100057614 cites W2019165625 @default.
• W2100057614 cites W2024890702 @default.
• W2100057614 cites W2026638336 @default.
• W2100057614 cites W2026766527 @default.
• W2100057614 cites W2028015487 @default.
• W2100057614 cites W2028967650 @default.
• W2100057614 cites W2036990500 @default.
• W2100057614 cites W2038267058 @default.
• W2100057614 cites W2044946913 @default.
• W2100057614 cites W2046599354 @default.
• W2100057614 cites W2055182510 @default.
• W2100057614 cites W2062792722 @default.
• W2100057614 cites W2068462651 @default.
• W2100057614 cites W2068525621 @default.
• W2100057614 cites W2072252524 @default.
• W2100057614 cites W2079763071 @default.
• W2100057614 cites W2086660631 @default.
• W2100057614 cites W2090316565 @default.
• W2100057614 cites W2105645345 @default.
• W2100057614 cites W2111616120 @default.
• W2100057614 cites W2113514561 @default.
• W2100057614 cites W2116474322 @default.
• W2100057614 cites W2117158343 @default.
• W2100057614 cites W2136060117 @default.
• W2100057614 cites W2139622987 @default.
• W2100057614 cites W2141349182 @default.
• W2100057614 cites W2148735872 @default.
• W2100057614 cites W2159316863 @default.
• W2100057614 cites W2166714856 @default.
• W2100057614 cites W2169729296 @default.
• W2100057614 cites W229404243 @default.
• W2100057614 cites W2306732009 @default.
• W2100057614 cites W2331980353 @default.
• W2100057614 cites W2404280758 @default.
• W2100057614 cites W2487439801 @default.
• W2100057614 cites W2529858948 @default.
• W2100057614 cites W264166021 @default.
• W2100057614 cites W40425401 @default.
• W2100057614 cites W4293247451 @default.
• W2100057614 cites W4321429334 @default.
• W2100057614 cites W53557829 @default.
• W2100057614 doi "https://doi.org/10.1042/bj20080292" @default.
• W2100057614 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18459942" @default.
• W2100057614 hasPublicationYear "2008" @default.
• W2100057614 type Work @default.
• W2100057614 sameAs 2100057614 @default.
• W2100057614 citedByCount "17" @default.
• W2100057614 countsByYear W21000576142012 @default.
• W2100057614 countsByYear W21000576142013 @default.
• W2100057614 countsByYear W21000576142014 @default.
• W2100057614 countsByYear W21000576142015 @default.
• W2100057614 countsByYear W21000576142016 @default.
• W2100057614 countsByYear W21000576142019 @default.
• W2100057614 countsByYear W21000576142020 @default.
• W2100057614 countsByYear W21000576142023 @default.
• W2100057614 crossrefType "journal-article" @default.
• W2100057614 hasAuthorship W2100057614A5041630420 @default.
• W2100057614 hasAuthorship W2100057614A5057386212 @default.
• W2100057614 hasAuthorship W2100057614A5080724123 @default.
• W2100057614 hasAuthorship W2100057614A5090863272 @default.
• W2100057614 hasBestOaLocation W21000576142 @default.
• W2100057614 hasConcept C104317684 @default.
• W2100057614 hasConcept C143065580 @default.
• W2100057614 hasConcept C181199279 @default.
• W2100057614 hasConcept C2777576037 @default.
• W2100057614 hasConcept C2777910421 @default.
• W2100057614 hasConcept C2778163477 @default.
• W2100057614 hasConcept C2778718757 @default.
• W2100057614 hasConcept C2779222958 @default.
• W2100057614 hasConcept C2779969316 @default.
• W2100057614 hasConcept C2781264208 @default.
• W2100057614 hasConcept C55493867 @default.
• W2100057614 hasConcept C86803240 @default.
• W2100057614 hasConceptScore W2100057614C104317684 @default.
• W2100057614 hasConceptScore W2100057614C143065580 @default.
• W2100057614 hasConceptScore W2100057614C181199279 @default.

• next