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• W2100059140 endingPage "C89" @default.
• W2100059140 startingPage "C78" @default.
• W2100059140 abstract "Nephrolithiasis remains a major health problem in Western countries. Seventy to 80% of kidney stones are composed of calcium oxalate, and small changes in urinary oxalate affect risk of kidney stone formation. Intestinal oxalate secretion mediated by the anion exchanger SLC26A6 plays an essential role in preventing hyperoxaluria and calcium oxalate nephrolithiasis, indicating that understanding the mechanisms regulating intestinal oxalate transport is critical for management of hyperoxaluria. Purinergic signaling modulates several intestinal processes through pathways including PKC activation, which we previously found to inhibit Slc26a6 activity in mouse duodenal tissue. We therefore examined whether purinergic stimulation with ATP and UTP affects oxalate transport by human intestinal Caco-2-BBe (C2) cells. We measured [¹⁴C]oxalate uptake in the presence of an outward Cl⁻ gradient as an assay of Cl⁻/oxalate exchange activity, ≥50% of which is mediated by SLC26A6. We found that ATP and UTP significantly inhibited oxalate transport by C2 cells, an effect blocked by the PKC inhibitor Gö-6983. Utilizing pharmacological agonists and antagonists, as well as PKC-δ knockdown studies, we observed that ATP inhibits oxalate transport through the P2Y₂ receptor, PLC, and PKC-δ. Biotinylation studies showed that ATP inhibits oxalate transport by lowering SLC26A6 surface expression. These findings are of potential relevance to pathophysiology of inflammatory bowel disease-associated hyperoxaluria, where supraphysiological levels of ATP/UTP are expected and overexpression of the P2Y₂ receptor has been reported. We conclude that ATP and UTP inhibit oxalate transport by lowering SLC26A6 surface expression in C2 cells through signaling pathways including the P2Y₂ purinergic receptor, PLC, and PKC-δ." @default.
• W2100059140 created "2016-06-24" @default.
• W2100059140 creator A5011446385 @default.
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• W2100059140 date "2013-07-01" @default.
• W2100059140 modified "2023-10-18" @default.
• W2100059140 title "Extracellular nucleotides inhibit oxalate transport by human intestinal Caco-2-BBe cells through PKC-δ activation" @default.
• W2100059140 cites W1532386517 @default.
• W2100059140 cites W1539795210 @default.
• W2100059140 cites W1556273428 @default.
• W2100059140 cites W1826133046 @default.
• W2100059140 cites W1896319706 @default.
• W2100059140 cites W1906804143 @default.
• W2100059140 cites W1963812065 @default.
• W2100059140 cites W1964008466 @default.
• W2100059140 cites W1967218829 @default.
• W2100059140 cites W1977884485 @default.
• W2100059140 cites W1978070601 @default.
• W2100059140 cites W1980760061 @default.
• W2100059140 cites W1982504166 @default.
• W2100059140 cites W1985650893 @default.
• W2100059140 cites W1987062630 @default.
• W2100059140 cites W1988606070 @default.
• W2100059140 cites W1998063951 @default.
• W2100059140 cites W1999353521 @default.
• W2100059140 cites W2002518345 @default.
• W2100059140 cites W2004295712 @default.
• W2100059140 cites W2008975884 @default.
• W2100059140 cites W2014272062 @default.
• W2100059140 cites W2019658196 @default.
• W2100059140 cites W2020880088 @default.
• W2100059140 cites W2025083211 @default.
• W2100059140 cites W2025904016 @default.
• W2100059140 cites W2027226283 @default.
• W2100059140 cites W2029718212 @default.
• W2100059140 cites W2033417601 @default.
• W2100059140 cites W2033969190 @default.
• W2100059140 cites W2034261640 @default.
• W2100059140 cites W2036530058 @default.
• W2100059140 cites W2038128655 @default.
• W2100059140 cites W2039936968 @default.
• W2100059140 cites W2048670782 @default.
• W2100059140 cites W2054924316 @default.
• W2100059140 cites W2061974142 @default.
• W2100059140 cites W2063597885 @default.
• W2100059140 cites W2064061221 @default.
• W2100059140 cites W2073023383 @default.
• W2100059140 cites W2075148529 @default.
• W2100059140 cites W2075171391 @default.
• W2100059140 cites W2076928712 @default.
• W2100059140 cites W2078064195 @default.
• W2100059140 cites W2080237684 @default.
• W2100059140 cites W2081152684 @default.
• W2100059140 cites W2085013466 @default.
• W2100059140 cites W2091180702 @default.
• W2100059140 cites W2091650990 @default.
• W2100059140 cites W2093316720 @default.
• W2100059140 cites W2095523245 @default.
• W2100059140 cites W2096548759 @default.
• W2100059140 cites W2096663326 @default.
• W2100059140 cites W2097455693 @default.
• W2100059140 cites W2099039975 @default.
• W2100059140 cites W2100853481 @default.
• W2100059140 cites W2104084275 @default.
• W2100059140 cites W2108112343 @default.
• W2100059140 cites W2108988197 @default.
• W2100059140 cites W2112089376 @default.
• W2100059140 cites W2113263764 @default.
• W2100059140 cites W2118744783 @default.
• W2100059140 cites W2125281084 @default.
• W2100059140 cites W2128875373 @default.
• W2100059140 cites W2144408702 @default.
• W2100059140 cites W2144998523 @default.
• W2100059140 cites W2151496784 @default.
• W2100059140 cites W2157901841 @default.
• W2100059140 cites W2159331385 @default.
• W2100059140 cites W2169715286 @default.
• W2100059140 cites W2170784049 @default.
• W2100059140 cites W2170857576 @default.
• W2100059140 cites W2203487265 @default.
• W2100059140 cites W2207061326 @default.
• W2100059140 cites W2243541242 @default.
• W2100059140 cites W2247034861 @default.
• W2100059140 cites W2269095518 @default.
• W2100059140 cites W2319970965 @default.
• W2100059140 cites W2398692990 @default.
• W2100059140 cites W2404185951 @default.
• W2100059140 cites W2429436704 @default.
• W2100059140 cites W2955403164 @default.
• W2100059140 cites W2146232553 @default.
• W2100059140 doi "https://doi.org/10.1152/ajpcell.00339.2012" @default.
• W2100059140 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3725516" @default.
• W2100059140 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23596171" @default.
• W2100059140 hasPublicationYear "2013" @default.
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