Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100061657> ?p ?o ?g. }
- W2100061657 endingPage "96" @default.
- W2100061657 startingPage "585" @default.
- W2100061657 abstract "DNA aneuploidy characterizes a proportion of malignant bone marrow (BM)-localized plasma cells in multiple myeloma (MM). This analysis shows that for most MM patients, circulating clonotypic B cells in MM are also hyperdiploid. Although all normal B cells and some malignant B cells are diploid, hyperdiploidy is likely to be exclusive to those that are malignant. Hyperdiploid MM B cells express CD34 and have clonotypic IgH transcripts, confirming them as part of the malignant clone. For MM, 92% (70/76) of patients had a DNA hyperdiploid subset [5-30% of peripheral blood mononuclear cells (PBMCs)] of CD19+ B cells. All CD19+ PBMCs in MM expressed CD19 and IgH variable diversity joining (VDJ) transcripts, confirming them as B cells. DNA aneuploid cells were undetectable in T or B lymphocytes from normal blood, spleen or thymus, or in blood from patients with B chronic lymphocytic leukemia. In MM, untreated patients had the highest DNA index (1.12). DNA hyperdiploid PBMCs were most frequent among untreated patients and were significantly reduced after chemotherapy. Diploid B cells were significantly more frequent after chemotherapy than at diagnosis. Of the hyperdiploid PBMCs, 81 +/- 3% expressed CD34 and CD19. In contrast to circulating CD34+ B cells, CD34- B cells in MM are diploid. In MM, unlike hyperdiploid PBMC B cells, hyperdiploid BM plasma cells lack both CD34 and CD19, suggesting that loss of CD34 correlates with differentiation and BM anchoring. In situ reverse transcription-PCR of the CD34+ (hyperdiploid) and CD34- (diploid) PBMC B-cell subsets was performed using patient-specific primers to amplify clonotypic IgH VDJ transcripts. Confirming previous work, CD34+ hyperdiploid MM PBMCs were clonotypic (86 +/- 5%). In contrast, CD34- diploid MM PBMCs had few monoclonal cells (4.8 +/- 2%). The lack of hyperdiploidy, together with the relative absence of cells having clonotypic transcripts, suggests these polyclonal CD34- B cells are normal. After culture in colchicine to arrest mitosis, hyperdiploid B cells were reduced and MM B cells accumulated in a diploid G2-M, suggesting that hyperdiploid in MM may represent a transient S-phase arrest rather than an aneuploid G0 phase. The DNA hyperdiploidy of CD34+ clonotypic B cells suggests these cells may be clinically important constituents of the myeloma clone and that they may play a direct role in the spread of myeloma." @default.
- W2100061657 created "2016-06-24" @default.
- W2100061657 creator A5006238796 @default.
- W2100061657 creator A5051445065 @default.
- W2100061657 creator A5053030748 @default.
- W2100061657 creator A5058237113 @default.
- W2100061657 creator A5073964632 @default.
- W2100061657 creator A5054476188 @default.
- W2100061657 date "2000-02-01" @default.
- W2100061657 modified "2023-09-23" @default.
- W2100061657 title "In multiple myeloma, circulating hyperdiploid B cells have clonotypic immunoglobulin heavy chain rearrangements and may mediate spread of disease." @default.
- W2100061657 cites W1445520737 @default.
- W2100061657 cites W146026460 @default.
- W2100061657 cites W146271095 @default.
- W2100061657 cites W1552273402 @default.
- W2100061657 cites W1576668137 @default.
- W2100061657 cites W1591596049 @default.
- W2100061657 cites W1598429767 @default.
- W2100061657 cites W179300038 @default.
- W2100061657 cites W187389121 @default.
- W2100061657 cites W1976017022 @default.
- W2100061657 cites W1976963122 @default.
- W2100061657 cites W1985170347 @default.
- W2100061657 cites W1985599774 @default.
- W2100061657 cites W1989169182 @default.
- W2100061657 cites W1998378792 @default.
- W2100061657 cites W2009364294 @default.
- W2100061657 cites W2013344838 @default.
- W2100061657 cites W2025392360 @default.
- W2100061657 cites W2042729968 @default.
- W2100061657 cites W2057993986 @default.
- W2100061657 cites W207383659 @default.
- W2100061657 cites W2087869106 @default.
- W2100061657 cites W2090248921 @default.
- W2100061657 cites W2113424017 @default.
- W2100061657 cites W2120218288 @default.
- W2100061657 cites W2126009525 @default.
- W2100061657 cites W2128098949 @default.
- W2100061657 cites W2150946292 @default.
- W2100061657 cites W2160757691 @default.
- W2100061657 cites W2165788078 @default.
- W2100061657 cites W2170045209 @default.
- W2100061657 cites W2285037228 @default.
- W2100061657 cites W2301504565 @default.
- W2100061657 cites W2316479316 @default.
- W2100061657 cites W2344721285 @default.
- W2100061657 cites W2373939791 @default.
- W2100061657 cites W2399383749 @default.
- W2100061657 cites W2408688530 @default.
- W2100061657 cites W2409125979 @default.
- W2100061657 cites W2415542453 @default.
- W2100061657 cites W2415781863 @default.
- W2100061657 cites W2417748069 @default.
- W2100061657 cites W2418745383 @default.
- W2100061657 cites W2420665477 @default.
- W2100061657 cites W2428771541 @default.
- W2100061657 cites W3111493210 @default.
- W2100061657 cites W67635181 @default.
- W2100061657 cites W68080126 @default.
- W2100061657 cites W8239046 @default.
- W2100061657 cites W2333363976 @default.
- W2100061657 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10690543" @default.
- W2100061657 hasPublicationYear "2000" @default.
- W2100061657 type Work @default.
- W2100061657 sameAs 2100061657 @default.
- W2100061657 citedByCount "12" @default.
- W2100061657 countsByYear W21000616572012 @default.
- W2100061657 countsByYear W21000616572016 @default.
- W2100061657 countsByYear W21000616572018 @default.
- W2100061657 countsByYear W21000616572019 @default.
- W2100061657 countsByYear W21000616572020 @default.
- W2100061657 crossrefType "journal-article" @default.
- W2100061657 hasAuthorship W2100061657A5006238796 @default.
- W2100061657 hasAuthorship W2100061657A5051445065 @default.
- W2100061657 hasAuthorship W2100061657A5053030748 @default.
- W2100061657 hasAuthorship W2100061657A5054476188 @default.
- W2100061657 hasAuthorship W2100061657A5058237113 @default.
- W2100061657 hasAuthorship W2100061657A5073964632 @default.
- W2100061657 hasConcept C10205521 @default.
- W2100061657 hasConcept C137061746 @default.
- W2100061657 hasConcept C153911025 @default.
- W2100061657 hasConcept C159654299 @default.
- W2100061657 hasConcept C202751555 @default.
- W2100061657 hasConcept C203014093 @default.
- W2100061657 hasConcept C2778453870 @default.
- W2100061657 hasConcept C2778957590 @default.
- W2100061657 hasConcept C2780007613 @default.
- W2100061657 hasConcept C28328180 @default.
- W2100061657 hasConcept C502942594 @default.
- W2100061657 hasConcept C54355233 @default.
- W2100061657 hasConcept C552990157 @default.
- W2100061657 hasConcept C81089528 @default.
- W2100061657 hasConcept C86803240 @default.
- W2100061657 hasConcept C95444343 @default.
- W2100061657 hasConceptScore W2100061657C10205521 @default.
- W2100061657 hasConceptScore W2100061657C137061746 @default.
- W2100061657 hasConceptScore W2100061657C153911025 @default.
- W2100061657 hasConceptScore W2100061657C159654299 @default.