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• W2100061987 abstract "Article1 September 2003free access Functional analysis of C-TAK1 substrate binding and identification of PKP2 as a new C-TAK1 substrate Jürgen Müller Jürgen Müller Regulation of Cell Growth Laboratory, Center for Cancer Research, NCI-Frederick, PO Box B, Frederick, MD, 21702 USA Search for more papers by this author Daniel A. Ritt Daniel A. Ritt Regulation of Cell Growth Laboratory, Center for Cancer Research, NCI-Frederick, PO Box B, Frederick, MD, 21702 USA Search for more papers by this author Terry D. Copeland Terry D. Copeland Regulation of Cell Growth Laboratory, Center for Cancer Research, NCI-Frederick, PO Box B, Frederick, MD, 21702 USA Search for more papers by this author Deborah K. Morrison Corresponding Author Deborah K. Morrison Regulation of Cell Growth Laboratory, Center for Cancer Research, NCI-Frederick, PO Box B, Frederick, MD, 21702 USA Search for more papers by this author Jürgen Müller Jürgen Müller Regulation of Cell Growth Laboratory, Center for Cancer Research, NCI-Frederick, PO Box B, Frederick, MD, 21702 USA Search for more papers by this author Daniel A. Ritt Daniel A. Ritt Regulation of Cell Growth Laboratory, Center for Cancer Research, NCI-Frederick, PO Box B, Frederick, MD, 21702 USA Search for more papers by this author Terry D. Copeland Terry D. Copeland Regulation of Cell Growth Laboratory, Center for Cancer Research, NCI-Frederick, PO Box B, Frederick, MD, 21702 USA Search for more papers by this author Deborah K. Morrison Corresponding Author Deborah K. Morrison Regulation of Cell Growth Laboratory, Center for Cancer Research, NCI-Frederick, PO Box B, Frederick, MD, 21702 USA Search for more papers by this author Author Information Jürgen Müller1, Daniel A. Ritt1, Terry D. Copeland1 and Deborah K. Morrison 1 1Regulation of Cell Growth Laboratory, Center for Cancer Research, NCI-Frederick, PO Box B, Frederick, MD, 21702 USA *Corresponding author. E-mail: [email protected] The EMBO Journal (2003)22:4431-4442https://doi.org/10.1093/emboj/cdg426 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Cdc25C-associated kinase 1 (C-TAK1) has been implicated in cell cycle regulation and Ras signaling through its interactions with two putative substrates, the Cdc25C phosphatase and the MAPK scaffold KSR1. Here, we identify sequence motifs required for stable C-TAK1 association and substrate phosphorylation. Using a mutational approach to disrupt binding of C-TAK1 to KSR1 and Cdc25C, we demonstrate that C-TAK1 contributes to the regulation of these proteins in vivo through the generation of 14-3-3-binding sites. KSR1 proteins defective in C-TAK1 binding had severely reduced phosphorylation at the 14-3-3-binding site in vivo, were constitutively localized to the plasma membrane and had increased biological activity. Disruption of the Cdc25C–C-TAK1 interaction resulted in reduced 14-3-3-binding site phosphorylation and nuclear accumulation of Cdc25C in interphase cells. Finally, utilizing the acquired C-TAK1 binding and substrate phosphorylation data, we identify plakophilin 2 (PKP2) as a novel C-TAK1 substrate. Phosphorylation of PKP2 by C-TAK1 also generates a 14-3-3-binding site that influences PKP2 localization. These findings underscore the importance of C-TAK1 as a regulator of 14-3-3 binding and protein localization. Introduction Intracellular protein trafficking plays an important role in the regulation of many cellular processes, including signal transduction, cell cycle regulation and apoptosis. In these processes, key components are often maintained as inactive factors in a different subcellular compartment from where they exert their effects. Only in response to the appropriate cellular cues do these molecules localize to their site of action. Two important mechanisms by which protein localization can be modulated are through protein interactions and phosphorylation events. A striking example of how protein interactions can affect localization is demonstrated by the Ras GTPase. In response to many extracellular stimuli, conversion of membrane-bound Ras to its GTP-bound state mediates the translocation of key cytoplasmic effectors, such as the Raf-1 kinase, to the cell surface (Marshall, 1996). Likewise, phosphorylation events can influence the movement of proteins from one cellular compartment to another. This mode of regulation is well illustrated by the binding of cytoplasmic SH2- and PTB-containing molecules to phosphotyrosine residues found on receptor tyrosine kinases at the cell surface (Yaffe, 2002b). More recently, it has become apparent that phosphorylation-dependent binding of 14-3-3 molecules also plays a key role in regulating intracellular protein movement. 14-3-3 family members are highly conserved dimeric proteins that bind mainly to phosphoserine motifs (Muslin et al., 1996; Yaffe et al., 1997). The primary manner by which 14-3-3 affects protein localization is through molecular interference, in that the binding of 14-3-3 directly alters the functioning of nearby subcellular targeting sequences found on the binding partner (Muslin and Xing, 2000; Yaffe, 2002a). 14-3-3 has been shown to modulate the nuclear/cytoplasmic shuttling of numerous proteins, including forkhead (Brunet et al., 1999), Cdc25C (Kumagai and Dunphy, 1999; Yang et al., 1999; Graves et al., 2001) and histone deacetylase (Grozinger and Schreiber, 2000; Kao et al., 2001). Similarly, 14-3-3 has been found to play a regulatory role in the cytoplasmic/membrane shuttling of proteins, such as the Raf-1 kinase (Jaumot and Hancock, 2001; Kubicek et al., 2002; Light et al., 2002) and the mitogen-activated protein kinase (MAPK) scaffolding protein KSR1 (Müller et al., 2001). With respect to KSR1, the binding of 14-3-3 molecules to two phosphoserine residues (S297 and S392) is critical for maintaining the cytoplasmic localization of the KSR1 complex in quiescent cells (Müller et al., 2001). In response to stimuli, the phosphorylation state of S392 is reduced, releasing 14-3-3 from this site. The removal of 14-3-3 appears to expose the cysteine-rich C1 domain of KSR1, which then directs the translocation of KSR1 to the plasma membrane, where it facilitates signal transmission from Raf-1 to MEK and MAPK (Müller et al., 2001; Zhou et al., 2002). In searching for kinases that might regulate the phosphorylation state of KSR1, we recently found that the protein kinase Cdc25C-associated kinase 1 (C-TAK1) is a constitutive component of the KSR1 complex (Müller et al., 2001). KSR1 itself is a substrate of C-TAK1 in vitro, and the KSR1 residue phosphorylated by C-TAK1 is the S392/14-3-3-binding site. C-TAK1 is a member of the EMK/MARK/Par1 kinase family and was first cloned based on its ability to associate with and phosphorylate Cdc25C (Ogg et al., 1994; Peng et al., 1998). Subsequently, the tyrosine phosphatase PTPH1 was found to be phosphorylated by C-TAK1 in vitro (Zhang et al., 1997). Strikingly, for all known substrates of C-TAK1 (KSR1, Cdc25C and PTPH1), the residue phosphorylated by C-TAK1 serves as a 14-3-3-binding site. To determine if the regulation of 14-3-3 binding is a general feature of C-TAK1 function and to uncover other proteins that might be substrates of C-TAK1, we initiated a mutagenesis study to define sequence motifs required for C-TAK1 binding and substrate phosphorylation. Here we report that a hydrophobic residue found at the +5 position relative to the site phosphorylated by C-TAK1 is critical for the stable association of C-TAK1 with its previously defined substrates Cdc25C and KSR1. Moreover, we find that hydrophobic residues at the −5, +1 and +5 positions as well as an arginine at the −3 position are required for optimal C-TAK1 phosphorylation. Analysis of KSR1 and Cdc25C proteins defective in C-TAK1 binding revealed the involvement of C-TAK1 in regulating the phosphorylation state and subcellular localization of these proteins, findings confirmed by overexpression of a dominant-inhibitory C-TAK1 protein. Finally, by searching protein databases with the acquired C-TAK1 binding and substrate phosphorylation data, we identify plakophilin 2 (PKP2) as a novel C-TAK1 substrate and show that C-TAK1-mediated phosphorylation of PKP2 generates a 14-3-3-binding site, which in turn influences the intracellular localization of PKP2. Results Defining KSR1 sequence(s) required for C-TAK1 binding C-TAK1 has been shown to stably associate with two of its substrate proteins, KSR1 and Cdc25C. Experiments analyzing the binding of C-TAK1 to various KSR1 and Cdc25C deletion mutants have revealed that the interaction with C-TAK1 requires residues 377–424 of murine KSR1 (Müller et al., 2001; data not shown) and residues 200–256 of human Cdc25C (Peng et al., 1998). When these regions of KSR1 and Cdc25C are compared, several areas of amino acid similarity can be observed (Figure 1A). In addition to the C-TAK1 target phosphorylation site that confers 14-3-3 binding (S392 for KSR1 and S216 of Cdc25C), these regions contain a cluster of lysine residues, proline residues found in specific sequence contexts [PxxR/K; a hydrophobic residue (Φ)PK] and hydrophobic residues located at similar positions surrounding the target phosphorylation site. To determine whether any of these amino acids are required for the interaction with C-TAK1, we first generated KSR1 proteins that have mutations in these sites. For this analysis, the mutations were incorporated into a KSR1 protein encoding the N-terminal 424 amino acids of KSR1 (KSR1/N′424), as this protein contains the binding site for C-TAK1, but not the interaction sites for many of the known KSR1-associated proteins, such as Raf-1, MEK, ERK, Hsp90 and Cdc37 (Morrison, 2001). The KSR1 mutants were then transiently expressed in Cos cells and examined for their ability to interact with endogenous C-TAK1. As shown in Figure 1B, C-TAK1 strongly associated with both the wild-type N′424 protein and the S392A mutant, indicating that the target site of phosphorylation is not required for C-TAK1 binding. In addition, the KSR1–C-TAK1 interaction was not altered by mutation of the lysine cluster, the proline motifs or the L383 and V401 hydrophobic residues. Strikingly, while C-TAK1 binding was somewhat reduced for the L387A mutant, little to no C-TAK1 was associated with the I397A mutant, indicating the importance of this residue for the stable interaction between KSR1 and C-TAK1. Figure 1.KSR1 residue(s) required for C-TAK1 binding. (A) Amino acids 201–249 of Cdc25C and 377–424 of KSR1. Regions of amino acid similarity are depicted in the following manner: the cluster of lysine residues is underlined, conserved hydrophobic residues are highlighted, and proline residues found in specific sequence contexts (PxxR and ΦPK) are indicated with asterisks. The serine residue phosphorylated by C-TAK1 is denoted with an arrow, and the 14-3-3-binding motif (RxxSxP) is boxed. (B and C) Wild-type and KSR1/N′424 mutant proteins were immunoprecipitated from Cos cell lysates, and the immune complexes examined by immunoblot analysis using C-TAK1 and Pyo antibodies. Download figure Download PowerPoint Defining KSR1 sequences required for C-TAK1-mediated phosphorylation Due to the close proximity of the I397 residue to the site phosphorylated by C-TAK1, we next performed experiments to determine whether this or other residues surrounding the S392 phosphoacceptor site are required for recognition of KSR1 as a C-TAK1 substrate. For these experiments, a series of KSR1 N′424 proteins was generated in which each residue from amino acid 387 to 398 was mutated to alanine. The mutant proteins were expressed in Cos cells and affinity purified from cell lysates under conditions known to disrupt KSR1 protein interactions (Müller et al., 2001). The purified mutants were then incubated in vitro with purified C-TAK1 in the presence of [γ-32P]ATP. The labeled proteins were isolated, digested with trypsin, and analyzed for S392 phosphorylation by high-pressure liquid chromatography (HPLC) (Figure 2A and B). In these assays, phosphorylation of wild-type N′424 was only observed in the presence of kinase-active C-TAK1, indicating that the purified KSR1 and C-TAK1 proteins did not contain contaminating kinase activities. Wild-type N′424 was phosphorylated efficiently by C-TAK1 and, as expected, phosphorylation was abolished by mutation of the S392 site. In addition, mutation of R389 severely reduced S392 phosphorylation, consistent with the fact that C-TAK1 is a member of the CAMK group of kinases, whose consensus phosphorylation motif often includes a basic residue at the −3 position relative to the phosphoacceptor site (Pearson and Kemp, 1991; Songyang et al., 1996). Likewise, mutation of hydrophobic residues at the −5 (L387A), +1(V393A) and +5 (I397A) positions inhibited C-TAK1-mediated S392 phosphorylation, with mutation of the +5 site having the most severe effect. Further mutational analysis revealed that while any hydrophobic residue was tolerated at the +1 position, hydrophobic residues with aliphatic side chains (I, L and V) were preferred at the −5 and +5 positions (Figure 2C). When the L387A–N398A panel of KSR1/N′424 mutants were assayed for their ability to stably associate with C-TAK1, only mutations that altered the I397 site were found to significantly disrupt C-TAK1 binding (Figure 1C, and data not shown). Based on this analysis, we conclude that C-TAK1 is a basophilic kinase, and that hydrophobic residues surrounding the phosphoacceptor site, in particular at positions −5, +1 and +5, play a critical role in C-TAK1 substrate phosphorylation. Interestingly, other members of the CAMK group, such as SNF1, AMP-activated kinase (AMPK), calcium/calmodulin-dependent kinase I (CAMKI), Chk1 and Chk2, have also been found to have a strong preference for substrates containing hydrophobic residues at the −5 position and a weaker requirement for hydrophobic residues at the +1 position (Dale et al., 1995; Hutchins et al., 2000; O'Neill et al., 2002). However, C-TAK1 appears to be unique among CAMK members in its preference for a hydrophobic amino acid at the +5 position, which, significantly, is also the residue required for the stable interaction between KSR1 and C-TAK1. Figure 2.Identification of KSR1 residues required for C-TAK1 phosphorylation. (A) In vitro kinase assays were performed using purified wild-type C-TAK1 or kinase-negative C-TAK1 (C-TAK1KN) and 0.2 μg of the purified KSR1/N′424 proteins. The labeled KSR1 proteins were resolved by SDS–PAGE, isolated from the gel matrix and digested with trypsin. The tryptic phosphopeptides were then separated by reverse-phase HPLC. The profile of radioactivity collected in the HPLC column fractions is shown for wild-type, S392A and I397A KSR1/N′424 proteins. (B and C) Kinase assays and HPLC analysis were performed as in (A) and the radioactivity incorporated into S392 was quantitated. S392 phosphorylation of wild-type KSR1/N′424 was considered to be 100% and the level of S392 phosphorylation for each mutant was compared with wild-type. Download figure Download PowerPoint Residues of Cdc25C required for the C-TAK1 interaction To determine whether a hydrophobic residue at the +5 position relative to the C-TAK1 phosphoacceptor site is required for stable binding of C-TAK1 to other substrates, we examined what effect mutation of the analogous Cdc25C residue (L221) would have on the Cdc25C–C-TAK1 interaction (Figure 3A). Polyomavirus (Pyo)-tagged Cdc25C proteins were generated in which the S216 phosphorylation site (S216A), the lysine residues at positions 241, 242 and 243 (KKK/AAA), and the hydrophobic residue at position 221 (L221A) were mutated to alanine. When the ability of these proteins to associate with endogenous C-TAK1 was examined, we found that equivalent amounts of C-TAK1 were observed in the wild-type, S216A and KKK/AAA Cdc25C immune complexes; however, no C-TAK1 was detected in immunoprecipitates of L221A Cdc25C. Thus, as with KSR1, the hydrophobic residue at the +5 position relative to the C-TAK1 phosphoacceptor site plays a critical role in mediating the interaction between C-TAK1 and Cdc25C. Figure 3.Analysis of Cdc25C residues required for C-TAK1-mediated phosphorylation. (A) Cos cells were transfected with constructs encoding Pyo-tagged wild-type, S216A, L221A or K242A/K243A/K244A (KKK/AAA) Cdc25C. Proteins were immunoprecipitated from cell lysates, and the immune complexes were examined by immunoblot analysis using C-TAK1 and Pyo antibodies. (B) In vitro phosphorylation of purified Cdc25C proteins by purified C-TAK1 and Chk1. For each mutant, the phosphate incorporated into the S216 site was quantitated and compared with wild-type. Download figure Download PowerPoint To investigate whether the L221 residue is also required for the phosphorylation of Cdc25C by C-TAK1, in vitro kinase assays were performed using purified Cdc25C and C-TAK1 proteins (Figure 3B). For this analysis, wild-type Cdc25C and proteins containing a mutation in either S216, L221 or the hydrophobic residue at the −5 position (L211A) were examined. In addition, because the Chk1 kinase has also been found to phosphorylate Cdc25C on the S216 site (Peng et al., 1997; Sanchez et al., 1997), the Cdc25C proteins were assayed for their ability to be phosphorylated by purified Chk1. HPLC analysis of the labeled proteins revealed that both kinases were able to phosphorylate wild-type Cdc25C on S216. The L211A mutant was a poor substrate for either C-TAK1 or Chk1, indicating the importance of a hydrophobic residue at the −5 position for substrate phosphorylation by both kinases. In contrast, mutation of L221 only disrupted C-TAK1-mediated phosphorylation of S216. These findings are consistent with the unique requirement of C-TAK1 for a hydrophobic residue at the +5 position, and further demonstrate the importance of this residue for both C-TAK1 binding and substrate phosphorylation. Functional assessment of the KSR1–C-TAK1 interaction in vivo The identification of mutations that disrupt the interaction of C-TAK1 with its substrates allows us to address the functional importance of C-TAK1 binding in vivo. As shown above, mutation of the hydrophobic residue at the +5 position relative to the phosphoacceptor site severely disrupts the binding and in vitro phosphorylation of KSR1 and Cdc25C by C-TAK1; however, based upon known phosphorylation recognition motifs and data presented in Figure 3B, this mutation should not interfere with the potential ability of other basophilic kinases, such as members of the AGC and CAMK families, to phosphorylate these proteins. Therefore, to address the importance of C-TAK1 in mediating the phosphorylation of KSR1 on S392 in intact cells, we examined the in vivo phosphorylation state, subcellular localization and biological activity of a full-length KSR1 protein containing the I397A mutation. Previously, we have shown that KSR1 is constitutively phosphorylated on S392 in quiescent cells, and that mutation of the S392 site leads to loss of 14-3-3 binding, constitutive plasma membrane localization of KSR1 in cultured cells and enhanced Ras-dependent maturation of Xenopus oocytes (Cacace et al., 1999; Müller et al., 2001). As shown in Figure 4A, when the in vivo phosphorylation state of wild-type, S392A and I397A KSR1 proteins was examined by HPLC analysis, wild-type KSR1 was found to be phosphorylated on two major sites in quiescent cells, S297 (eluting in fraction 7) and S392 (eluting in fraction 24). Although mutation of I397 had no effect on S297 phosphorylation, phosphorylation of S392 was reduced ∼75%. Moreover, we found that this altered phosphorylation status was accompanied by constitutive localization of the I397A mutant to the plasma membrane in NIH-3T3 cells (Figure 4B), as well as accelerated Ras-dependent maturation kinetics in the Xenopus oocyte assay (Figure 4C). To address the role of C-TAK1 in S392 phosphorylation further, we next examined the in vivo phosphorylation state of wild-type KSR1 in cells overexpressing either wild-type C-TAK1 or a kinase-negative C-TAK1 protein, which has been shown to interfere with the activity of endogenous C-TAK1 (Sun et al., 2001). As depicted in Figure 4D, expression of the kinase-negative C-TAK1 specifically reduced phosphorylation of the S392 site. Taken together, these findings demonstrate that C-TAK1 is a primary in vivo kinase that phosphorylates KSR1 on S392. Figure 4.In vivo analysis of the KSR1–C-TAK1 interaction. (A) Quiescent Cos cells expressing wild-type, S392A or I397A KSR1 were labeled in vivo with [32P]orthophosphate. The labeled KSR1 proteins were isolated, digested with trypsin and examined by HPLC analysis. The arrow indicates the position of the peak containing the S392 peptide. It should be noted that the I397A substitution causes the S392 peptide to elute earlier in the HPLC gradient (as confirmed by sequence analysis). (B) NIH-3T3 cells were transfected with constructs encoding either wild-type, S392A or I397A KSR1. Localization of the KSR1 proteins was then determined by indirect immunofluorescence using the Pyo antibody. (C) Stage VI Xenopus oocytes were injected with RNAs encoding wild-type, S392A or I397A KSR1. Oocytes subsequently were injected with activated RasV12 RNA and scored for germinal vesicle breakdown (GVBD) 5 h following Ras injection. (D) Quiescent Cos cells co-expressing wild-type KSR1 and either wild-type C-TAK1 or kinase-negative C-TAK1 (C-TAK1KN) were labeled in vivo with [32P]orthophosphate and analyzed as described in (A). Download figure Download PowerPoint Disruption of C-TAK1 binding alters the in vivo phosphorylation state and subcellular localization of Cdc25C To examine whether C-TAK1 contributes to the phosphorylation of Cdc25C on S216 in vivo, we examined the phosphorylation state of wild-type, S216A or L221A Cdc25C proteins in quiescent interphase cells. HPLC analysis of the labeled proteins revealed that wild-type Cdc25C contains tryptic phosphopeptides that elute in fractions 24 and 26. Due to incomplete trypsin digestion at closely spaced lysine residues, K225 and K227, two S216-containing peptides are generated that elute in fractions 24 and 26. By sequence analysis, both fractions contain peptides phosphorylated at S216 and are not detected if the S216 site is mutated (Figure 5A). When the HPLC profile of L221A Cdc25C was examined, phosphorylation of S216 was found to be reduced ∼65%. In addition, overexpression of kinase-inactive C-TAK1 was also found to reduce the in vivo phosphorylation state of S216 (Figure 5C). Figure 5.In vivo analysis of the Cdc25C–C-TAK1 interaction. (A) Quiescent Cos cells expressing wild-type, S216A or L221A Cdc25C were labeled in vivo with [32P]orthophosphate. The labeled Cdc25C proteins were isolated, digested with trypsin and examined by HPLC analysis. (B) NIH-3T3 cells were transfected with constructs encoding Pyo-tagged wild-type, S216A or L221A Cdc25C. Localization of the Cdc25C proteins was then determined by indirect immunofluorescence using the Pyo antibody. (C) Quiescent Cos cells co-expressing wild-type Cdc25C and either wild-type C-TAK1 or kinase-negative C-TAK1 (C-TAK1KN) were labeled in vivo with [32P]orthophosphate and analyzed as described in (A). Download figure Download PowerPoint Because S216 phosphorylation and associated 14-3-3 binding has been shown to regulate the nuclear–cytoplasmic shuttling of Cdc25C (Peng et al., 1998; Kumagai and Dunphy, 1999; Yang et al., 1999; Graves et al., 2001), we next examined what effect disruption of the Cdc25C–C-TAK1 interaction would have on the subcellular localization of Cdc25C (Figure 5B). As expected, wild-type Cdc25C, which is phosphorylated on S216, was localized exclusively in the cytosol. In contrast, both the S216A and L221A mutants exhibited cytoplasmic and nuclear localization. Thus, the loss of C-TAK1 binding has the same effect on Cdc25C as does mutation of the S216 phosphorylation site, indicating that C-TAK1 contributes to the regulation of S216 phosphorylation and Cdc25C intracellular localization in quiescent interphase cells. Identification of plakophilin 2 as a new C-TAK1 substrate The above results suggest that the substrate recognition motif of C-TAK1 may be defined as ΦaxRxxS*ΦxxxΦa, where * is the site phosphorylated, x is any amino acid, Φa is a hydrophobic residue with aliphatic side chains and Φ is any hydrophobic amino acid. Moreover, our findings demonstrate that the hydrophobic residue at the +5 position is also required for the stable interaction of C-TAK1 with its targets KSR1 and Cdc25C. Therefore, to identify other potential in vivo substrates of C-TAK1, we searched the TrEMBL and Swiss-Prot databases for proteins that contain the following motif, ΦaxRxxSΦxxxΦa (Figure 6A). Expression constructs for several proteins identified in this search were obtained, including plakophilin 2 (PKP2), partitioning-defective 3-like protein (Par3L), platelet-type lipoxygenase 12 (LOXP), signal-induced proliferation-associated protein 1 (SPA1) and RalBP1-interacting protein (POB1). The proteins were then expressed in Cos cells and assessed for their ability to interact with C-TAK1 by co-immunoprecipitation assays (Figure 6B). KSR1 immune complexes served as a positive control for C-TAK1 binding, whereas immunoprecipitates of Raf-1 and MKK3 (proteins which do not contain the ΦaxRxxSΦxxxΦa motif) were included as negative controls. By immunoblot analysis, C-TAK1 was found to associate with KSR1 and both the full-length and N-terminal head domain (residues 1–348) of PKP2. As verification that C-TAK1 binding to PKP2 was mediated by the sequence identified in the motif search, we found that the interaction between C-TAK1 and PKP2 could be disrupted by mutation of the hydrophobic residue at the +5 position relative to the predicted phosphoacceptor site (V87A; Figure 6C). Figure 6.Binding of C-TAK1 to PKP2. (A) Sequences surrounding the known C-TAK1 phosphorylation sites of KSR1 (S392) and Cdc25C (S216) are shown. Also depicted are sequences from proteins containing the ΦaxRxxSΦxxxΦa motif, which were tested for C-TAK1 binding. (B) Cos cells were transfected with constructs encoding N′424 KSR1, POB1, Spa1, Par 3L, 12-LOX, MKK3, Raf-1 and either full-length or the N-terminal head domain of PKP2 (PKP2 N′). Proteins were immunoprecipitated from cell lysates, and the immune complexes were examined for the presence of C-TAK1. Lysates were probed with a mixture of antibodies recognizing the Pyo, FLAG, GFP, Myc and HA epitope tags to confirm the expression of the transfected proteins. (C) PKP2 proteins immunoprecipitated from Cos cells expressing wild-type, S82A or V87A PKP2 were examined by immunoblot analysis using C-TAK1 and FLAG antibodies. Download figure Download PowerPoint Next, to determine whether PKP2 is a substrate of C-TAK1, in vitro kinase assays were performed using affinity-purified PKP2 and C-TAK1. The phosphorylation state of PKP2 was then examined by HPLC analysis. As shown in Figure 7A, PKP2 was phosphorylated by C-TAK1 on a tryptic peptide that eluted in fraction 65 of the HPLC gradient. Through phosphoamino acid analysis and Edman degradation of the labeled peptide, the residue phosphorylated was determined to be S82 (the site predicted by the motif search). To investigate whether S82 is phosphorylated in intact cells and whether C-TAK1 contributes to the phosphorylation of S82, we examined the in vivo phosphorylation state of wild-type PKP2 and proteins mutated at either the S82 or V87 site (Figure 7A). By HPLC analysis, wild-type PKP2 contained a phosphopeptide eluting in fraction 65, which was not observed when a similar analysis was performed on the S82A mutant. In addition, phosphorylation of the fraction 65 peptide was reduced ∼72% for the PKP2 V87A mutant (Figure 7A) and was reduced ∼51% when wild-type PKP2 was expressed with kinase-negative C-TAK1 (Figure 7B). These findings identify S82 as a site of PKP2 phosphorylated in vivo and demonstrate that PKP2 is an authentic substrate of C-TAK1. Figure 7.PKP2 is a substrate of C-TAK1. (A) In vitro kinase assays were performed using purified C-TAK1 and purified PKP2 (top left panel). Wild-type, S82A and V87A PKP2 proteins expressed in Cos cells were labeled in vivo with [32P]orthophosphate (bottom left and right panels). The labeled PKP2 proteins were isolated, digested with trypsin and examined by HPLC analysis. Also shown is the sequence of the phosphorylated peptide eluting in fraction 65. (B) Quiescent Cos cells co-expressing wild-type PKP2 and either wild-type C-TAK1 or kinase-negative C-TAK1 (C-TAK1KN) were labeled in vivo with [32P]orthophosphate and analyzed as described in (A). Download figure Download PowerPoint Phosphorylation of S82 mediates 14-3-3 binding and influences the subcellular localization of PKP2 Given that the PKP2 S82 phosphorylation site is found within a canonical 14-3-3-binding motif (RSxS*xP; Yaffe et al., 1997), we next examined whether PKP2 interacts with 14-3-3. As shown in Figure 8A, PKP2 was found to co-immunoprecipitate with 14-3-3; however, this interaction could be disrupted by mutations that either destroy the S82 site (S82A) or severely reduce its phosphorylation (V87A), confirming that S82 is a functional 14-3-3-binding site. Figure 8.In vivo analysis of the PKP2–14-3-3 interaction. (A) Cos cells were transfected with constructs encoding 14-3-3 and wild-type, S82A or V87A PKP2. 14-3-3 proteins were immunoprecipitated from cell lysates, and" @default.
• W2100061987 created "2016-06-24" @default.
• W2100061987 creator A5000884417 @default.
• W2100061987 creator A5061515984 @default.
• W2100061987 creator A5067844076 @default.
• W2100061987 creator A5077524772 @default.
• W2100061987 date "2003-09-01" @default.
• W2100061987 modified "2023-09-25" @default.
• W2100061987 title "Functional analysis of C-TAK1 substrate binding and identification of PKP2 as a new C-TAK1 substrate" @default.
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