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- W2100062084 abstract "Until now a satisfactory method for electron microscopic (EM) detection of anterogradely transported horseradish peroxidase (HRP) in developing neural tissue, using sensitive chromogen tetramethylbenzidine (TMB), has not been described. Use of the stabilizing agent ammoniumheptamolybdate (AHM), in combination with a modified prolonged osmication [4 hr at pH 5.0 in 0.1 M phosphate buffer (PB)] made possible visualization of HRP-TMB-(AHM) reaction product at the ultrastructural level in outgrowing corticospinal tract (CST) fibers of young postnatal rat. This reaction product appeared to be very distinctive and clearly detectable, making ultrastructural identification of HRP-labeled outgrowing CST fibers in rat spinal cord rather easy. In addition, the procedure described in this report preserves the ultrastructural details of the developing neural tissue." @default.
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- W2100062084 date "1987-05-01" @default.
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- W2100062084 title "Ultrastructural visualization of anterogradely transported horseradish peroxidase in developing corticospinal tract of rat." @default.
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- W2100062084 doi "https://doi.org/10.1177/35.5.2435786" @default.
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