FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100067555> ?p ?o ?g. }

• W2100067555 endingPage "11793" @default.
• W2100067555 startingPage "11781" @default.
• W2100067555 abstract "We have prepared a series of undecadeoxynucleotides that contain changes in the functional group pattern present within the EcoRV recognition site - GATATC -. Oligonucleotides were synthesized on solid phase using normal and modified β-cyanoethylphosphoramidites and analyzed in steady state cleavage experiments with the EcoRV restriction endonuclease. The following groups appear to interact strongly with the enzyme, since their modification or substitution renders the oligonucleotides refractory to cleavage: the exocyclic NH 2 -groups of both A residues the N7 of the first A residue, the exocyclic NH 2 -group of the C residue and the CH 3 -groups of both T residues. The exocyclic NH-group of the G residue supports effective recognition, since its absence lowers the k cat of the cleavage reaction. The N7 of the second A residue and the C5 position of the C residue apparently are not recognized by EcoRV; their substitution by -CH- or modification with -Br or -CH 3 resp., does not considerably change the rate of cleavage. All oligonucleotides investigated compete with the unmodified substrate for binding to the enzyme. We conclude that EcoRV recognizes its substrate presumably through hydrogen bonds to the exocyclic NH 2 -group and the N7 of the first A residue, the exocyclic NH 2 -groups of the second A and the C residue, as well as through hydrophobic interactions with both T residues." @default.
• W2100067555 created "2016-06-24" @default.
• W2100067555 creator A5033630820 @default.
• W2100067555 creator A5060869360 @default.
• W2100067555 creator A5062346683 @default.
• W2100067555 creator A5088616795 @default.
• W2100067555 date "1988-01-01" @default.
• W2100067555 modified "2023-10-14" @default.
• W2100067555 title "Analysis of the recognition mechanism involved in the EcoRV catalyzed cleavage of DNA using modified oligodeoxynucleotides" @default.
• W2100067555 cites W1481640863 @default.
• W2100067555 cites W1483513249 @default.
• W2100067555 cites W1499727494 @default.
• W2100067555 cites W1499803760 @default.
• W2100067555 cites W1510656481 @default.
• W2100067555 cites W1525252873 @default.
• W2100067555 cites W1526228480 @default.
• W2100067555 cites W1526944695 @default.
• W2100067555 cites W1543979595 @default.
• W2100067555 cites W1553262587 @default.
• W2100067555 cites W1554753165 @default.
• W2100067555 cites W1558917577 @default.
• W2100067555 cites W1562354167 @default.
• W2100067555 cites W1578766181 @default.
• W2100067555 cites W1586613062 @default.
• W2100067555 cites W1594420006 @default.
• W2100067555 cites W1866477056 @default.
• W2100067555 cites W1966497102 @default.
• W2100067555 cites W1967894471 @default.
• W2100067555 cites W1968251916 @default.
• W2100067555 cites W1969058261 @default.
• W2100067555 cites W1976151690 @default.
• W2100067555 cites W1978302108 @default.
• W2100067555 cites W1986145587 @default.
• W2100067555 cites W1986586522 @default.
• W2100067555 cites W1987915639 @default.
• W2100067555 cites W1997972620 @default.
• W2100067555 cites W2000460754 @default.
• W2100067555 cites W2003003271 @default.
• W2100067555 cites W2007129105 @default.
• W2100067555 cites W2009257137 @default.
• W2100067555 cites W2011846105 @default.
• W2100067555 cites W2011904983 @default.
• W2100067555 cites W2014579362 @default.
• W2100067555 cites W2020455189 @default.
• W2100067555 cites W2025365677 @default.
• W2100067555 cites W2025680094 @default.
• W2100067555 cites W2028714877 @default.
• W2100067555 cites W2030191229 @default.
• W2100067555 cites W2037549763 @default.
• W2100067555 cites W2040548085 @default.
• W2100067555 cites W2041393770 @default.
• W2100067555 cites W2043317558 @default.
• W2100067555 cites W2044732762 @default.
• W2100067555 cites W2049101076 @default.
• W2100067555 cites W2050879305 @default.
• W2100067555 cites W2051001716 @default.
• W2100067555 cites W2054906109 @default.
• W2100067555 cites W2056625258 @default.
• W2100067555 cites W2057024685 @default.
• W2100067555 cites W2058743988 @default.
• W2100067555 cites W2067363689 @default.
• W2100067555 cites W2068039724 @default.
• W2100067555 cites W2071574309 @default.
• W2100067555 cites W2075467867 @default.
• W2100067555 cites W2084015439 @default.
• W2100067555 cites W2085005993 @default.
• W2100067555 cites W2089811070 @default.
• W2100067555 cites W2090962585 @default.
• W2100067555 cites W2093823983 @default.
• W2100067555 cites W2109623013 @default.
• W2100067555 cites W2113608488 @default.
• W2100067555 cites W2116999239 @default.
• W2100067555 cites W2125696406 @default.
• W2100067555 cites W2133042489 @default.
• W2100067555 cites W2151381566 @default.
• W2100067555 cites W2325098152 @default.
• W2100067555 cites W2407896957 @default.
• W2100067555 cites W2413543976 @default.
• W2100067555 cites W2418933884 @default.
• W2100067555 cites W3133882337 @default.
• W2100067555 cites W356413772 @default.
• W2100067555 doi "https://doi.org/10.1093/nar/16.24.11781" @default.
• W2100067555 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/339110" @default.
• W2100067555 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3062581" @default.
• W2100067555 hasPublicationYear "1988" @default.
• W2100067555 type Work @default.
• W2100067555 sameAs 2100067555 @default.
• W2100067555 citedByCount "53" @default.
• W2100067555 countsByYear W21000675552012 @default.
• W2100067555 countsByYear W21000675552013 @default.
• W2100067555 countsByYear W21000675552014 @default.
• W2100067555 countsByYear W21000675552015 @default.
• W2100067555 countsByYear W21000675552017 @default.
• W2100067555 crossrefType "journal-article" @default.
• W2100067555 hasAuthorship W2100067555A5033630820 @default.
• W2100067555 hasAuthorship W2100067555A5060869360 @default.
• W2100067555 hasAuthorship W2100067555A5062346683 @default.
• W2100067555 hasAuthorship W2100067555A5088616795 @default.

• next