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- W2100068599 abstract "Several treatments were investigated in an attempt to increase the proportion of vanadate-sensitive proton pumping activity derived from membrane fractions of Ricinus cotyledons. The most successful procedure involved KI treatment of the microsomal fraction followed by solubilization with 1.25% (w/v) octylglucoside and reconstitution into phosopholipid liposomes. KI treatment of the microsomal fraction resulted in an increase in the ATPase sensitivity to vanadate. Reconstitution was carried out by a dilution method and the existence of ATP-driven H+-transport across the proteoliposomes was demonstrated by quinacrine fluorescence quenching. The quenching was gramicidin reversible and strongly inhibited by vanadate, ER B and PCMBS. Less inhibition was observed in the presence of NEM. Fusicoccin and sucrose did not have marked effects on H+ -transport." @default.
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- W2100068599 date "1989-01-01" @default.
- W2100068599 modified "2023-09-29" @default.
- W2100068599 title "Solubilization, Reconstitution and Characterization of Vanadate-sensitive, ATP-driven H<sup>+</sup>-transport from cotyledons of<i>Ricinus communis</i>" @default.
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- W2100068599 doi "https://doi.org/10.1093/jxb/40.11.1215" @default.
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