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• W2100078622 endingPage "e1002905" @default.
• W2100078622 startingPage "e1002905" @default.
• W2100078622 abstract "Epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor-α (PDGFRα) were reported to mediate entry of HCMV, including HCMV lab strain AD169. AD169 cannot assemble gH/gL/UL128–131, a glycoprotein complex that is essential for HCMV entry into biologically important epithelial cells, endothelial cells, and monocyte-macrophages. Given this, it appeared incongruous that EGFR and PDGFRα play widespread roles in HCMV entry. Thus, we investigated whether PDGFRα and EGFR could promote entry of wild type HCMV strain TR. EGFR did not promote HCMV entry into any cell type. PDGFRα–transduction of epithelial and endothelial cells and several non-permissive cells markedly enhanced HCMV TR entry and surprisingly, promoted entry of HCMV mutants lacking gH/gL/UL128–131 into epithelial and endothelial cells. Entry of HCMV was not blocked by a panel of PDGFRα antibodies or the PDGFR ligand in fibroblasts, epithelial, or endothelial cells or by shRNA silencing of PDGFRα in epithelial cells. Moreover, HCMV glycoprotein induced cell-cell fusion was not increased when PDGFRα was expressed in cells. Together these results suggested that HCMV does not interact directly with PDGFRα. Instead, the enhanced entry produced by PDGFRα resulted from a novel entry pathway involving clathrin-independent, dynamin-dependent endocytosis of HCMV followed by low pH-independent fusion. When PDGFRα was expressed in cells, an HCMV lab strain escaped endosomes and tegument proteins reached the nucleus, but without PDGFRα virions were degraded. By contrast, wild type HCMV uses another pathway to enter epithelial cells involving macropinocytosis and low pH-dependent fusion, a pathway that lab strains (lacking gH/gL/UL128–131) cannot follow. Thus, PDGFRα does not act as a receptor for HCMV but increased PDGFRα alters cells, facilitating virus entry by an abnormal pathway. Given that PDGFRα increased infection of some cells to 90%, PDGFRα may be very useful in overcoming inefficient HCMV entry (even of lab strains) into the many difficult-to-infect cell types." @default.
• W2100078622 created "2016-06-24" @default.
• W2100078622 creator A5000357300 @default.
• W2100078622 creator A5022477381 @default.
• W2100078622 creator A5031083038 @default.
• W2100078622 creator A5073661736 @default.
• W2100078622 creator A5084984241 @default.
• W2100078622 date "2012-09-13" @default.
• W2100078622 modified "2023-10-16" @default.
• W2100078622 title "PDGF Receptor-α Does Not Promote HCMV Entry into Epithelial and Endothelial Cells but Increased Quantities Stimulate Entry by an Abnormal Pathway" @default.
• W2100078622 cites W1253550149 @default.
• W2100078622 cites W1516223106 @default.
• W2100078622 cites W1589492593 @default.
• W2100078622 cites W1596555978 @default.
• W2100078622 cites W1604517259 @default.
• W2100078622 cites W1638089104 @default.
• W2100078622 cites W1695142101 @default.
• W2100078622 cites W1715558547 @default.
• W2100078622 cites W1790420597 @default.
• W2100078622 cites W1864251807 @default.
• W2100078622 cites W1919588248 @default.
• W2100078622 cites W1973640489 @default.
• W2100078622 cites W1993828483 @default.
• W2100078622 cites W1999722506 @default.
• W2100078622 cites W2005017965 @default.
• W2100078622 cites W2007134676 @default.
• W2100078622 cites W2007639993 @default.
• W2100078622 cites W2008486572 @default.
• W2100078622 cites W2015704490 @default.
• W2100078622 cites W2018011972 @default.
• W2100078622 cites W2029345949 @default.
• W2100078622 cites W2033207340 @default.
• W2100078622 cites W2035256912 @default.
• W2100078622 cites W2043674872 @default.
• W2100078622 cites W2043919474 @default.
• W2100078622 cites W2045249072 @default.
• W2100078622 cites W2051245442 @default.
• W2100078622 cites W2052473929 @default.
• W2100078622 cites W2065505211 @default.
• W2100078622 cites W2068366518 @default.
• W2100078622 cites W2073898372 @default.
• W2100078622 cites W2075517421 @default.
• W2100078622 cites W2078319463 @default.
• W2100078622 cites W2085280361 @default.
• W2100078622 cites W2086769942 @default.
• W2100078622 cites W2089974427 @default.
• W2100078622 cites W2092071546 @default.
• W2100078622 cites W2094390105 @default.
• W2100078622 cites W2096597082 @default.
• W2100078622 cites W2096963698 @default.
• W2100078622 cites W2099806165 @default.
• W2100078622 cites W2100034740 @default.
• W2100078622 cites W2103010723 @default.
• W2100078622 cites W2103738194 @default.
• W2100078622 cites W2104124112 @default.
• W2100078622 cites W2104219682 @default.
• W2100078622 cites W2107655439 @default.
• W2100078622 cites W2118578639 @default.
• W2100078622 cites W2120357172 @default.
• W2100078622 cites W2121749962 @default.
• W2100078622 cites W2122513022 @default.
• W2100078622 cites W2124944848 @default.
• W2100078622 cites W2128746059 @default.
• W2100078622 cites W2132275721 @default.
• W2100078622 cites W2133392557 @default.
• W2100078622 cites W2134812039 @default.
• W2100078622 cites W2135275277 @default.
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• W2100078622 cites W2140404757 @default.
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• W2100078622 cites W2155214429 @default.
• W2100078622 cites W2157963288 @default.
• W2100078622 cites W2157973146 @default.
• W2100078622 cites W2165691323 @default.
• W2100078622 cites W2170651190 @default.
• W2100078622 cites W2170669821 @default.
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• W2100078622 doi "https://doi.org/10.1371/journal.ppat.1002905" @default.
• W2100078622 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3441672" @default.
• W2100078622 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23028311" @default.
• W2100078622 hasPublicationYear "2012" @default.
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