Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100082336> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W2100082336 abstract "Aim Susceptibility to rheumatoid arthritis (RA) has been shown to be associated with DRB1*04 and *01 alleles due to the presence of the QRRAA “shared epitope” in amino acids 70–74. However, despite both DRB1*01:01 and *01:02 having the QRRAA shared epitope, DRB1*01:01 is more strongly associated with RA. The purpose of this study was to determine if the binding of citrullinated and non-citrullinated peptides to DRB1*01:01, *01:02 and *01:03 (as a negative control) could be used to dissect the role of the shared epitope and other amino acids in RA susceptibility. Methods Binding of type II collagen 259–273 (CII), citrullinated and native vimentin 66–78 , and citrullinated and native α -enolase 11–25 was measured on cell lines expressing DRB1*01:01, *01:02 and *01:03 by flow cytometry. Single site mutagenesis was performed to examine how the differences between these alleles affect peptide binding and T cell responses. Results DRB1*01:01 exhibited an 8.2-fold preference for binding citrullinated vimentin 66–78 over its native form, compared to 7.8-fold for *01:02 and 0.68-fold (preference for binding native) for *01:03. DRB1*01:01 also exhibited a 2-fold preference for binding citrullinated α -enolase 11–25 over native, compared to 1.0-fold (no preference) for *01:02 and 0.49-fold (preference for native) for *01:03. In addition, DRB1*01:01 bound more type II collagen (CII) 259–273 (3.7-fold over background) than *01:02 (1.1-fold) and *01:03 (1.7-fold). Mutating G86 in DRB1*01:01 to the residue found in DRB1*01:02 (V86) abolished citrullinated α -enolase 11–25 and CII 259–273 binding and decreased citrullinated vimentin 66–78 binding (1.7-fold). Conclusion The difference in susceptibility between DRB1*01:01 and *01:02 for RA may be explained by the effect of position 86 on peptide binding. Download full-size image" @default.
- W2100082336 created "2016-06-24" @default.
- W2100082336 creator A5001645001 @default.
- W2100082336 creator A5022995100 @default.
- W2100082336 creator A5024774778 @default.
- W2100082336 creator A5032158017 @default.
- W2100082336 creator A5063613068 @default.
- W2100082336 date "2015-10-01" @default.
- W2100082336 modified "2023-09-26" @default.
- W2100082336 title "OR11 Comparison of arthritogenic peptide binding to DRB1*01:01 and DRB1*01:02" @default.
- W2100082336 doi "https://doi.org/10.1016/j.humimm.2015.07.031" @default.
- W2100082336 hasPublicationYear "2015" @default.
- W2100082336 type Work @default.
- W2100082336 sameAs 2100082336 @default.
- W2100082336 citedByCount "0" @default.
- W2100082336 crossrefType "journal-article" @default.
- W2100082336 hasAuthorship W2100082336A5001645001 @default.
- W2100082336 hasAuthorship W2100082336A5022995100 @default.
- W2100082336 hasAuthorship W2100082336A5024774778 @default.
- W2100082336 hasAuthorship W2100082336A5032158017 @default.
- W2100082336 hasAuthorship W2100082336A5063613068 @default.
- W2100082336 hasConcept C147447768 @default.
- W2100082336 hasConcept C153911025 @default.
- W2100082336 hasConcept C159654299 @default.
- W2100082336 hasConcept C185592680 @default.
- W2100082336 hasConcept C195616568 @default.
- W2100082336 hasConcept C203014093 @default.
- W2100082336 hasConcept C204232928 @default.
- W2100082336 hasConcept C2779281246 @default.
- W2100082336 hasConcept C515207424 @default.
- W2100082336 hasConcept C553184892 @default.
- W2100082336 hasConcept C55493867 @default.
- W2100082336 hasConcept C86803240 @default.
- W2100082336 hasConcept C8891405 @default.
- W2100082336 hasConceptScore W2100082336C147447768 @default.
- W2100082336 hasConceptScore W2100082336C153911025 @default.
- W2100082336 hasConceptScore W2100082336C159654299 @default.
- W2100082336 hasConceptScore W2100082336C185592680 @default.
- W2100082336 hasConceptScore W2100082336C195616568 @default.
- W2100082336 hasConceptScore W2100082336C203014093 @default.
- W2100082336 hasConceptScore W2100082336C204232928 @default.
- W2100082336 hasConceptScore W2100082336C2779281246 @default.
- W2100082336 hasConceptScore W2100082336C515207424 @default.
- W2100082336 hasConceptScore W2100082336C553184892 @default.
- W2100082336 hasConceptScore W2100082336C55493867 @default.
- W2100082336 hasConceptScore W2100082336C86803240 @default.
- W2100082336 hasConceptScore W2100082336C8891405 @default.
- W2100082336 hasLocation W21000823361 @default.
- W2100082336 hasOpenAccess W2100082336 @default.
- W2100082336 hasPrimaryLocation W21000823361 @default.
- W2100082336 hasRelatedWork W1558112754 @default.
- W2100082336 hasRelatedWork W1571252385 @default.
- W2100082336 hasRelatedWork W1965909144 @default.
- W2100082336 hasRelatedWork W2008373775 @default.
- W2100082336 hasRelatedWork W2061304883 @default.
- W2100082336 hasRelatedWork W2063797375 @default.
- W2100082336 hasRelatedWork W2091766674 @default.
- W2100082336 hasRelatedWork W2107773953 @default.
- W2100082336 hasRelatedWork W2137926676 @default.
- W2100082336 hasRelatedWork W2139244097 @default.
- W2100082336 hasRelatedWork W2975593702 @default.
- W2100082336 hasRelatedWork W2234401885 @default.
- W2100082336 isParatext "false" @default.
- W2100082336 isRetracted "false" @default.
- W2100082336 magId "2100082336" @default.
- W2100082336 workType "article" @default.