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- W21000857 abstract "Rat liver somatogenic receptors have been characterized by gel permeation chromatography, sucrose density gradients in H2O and D2O and affinity cross-linking using 125I-bovine growth hormone as a specific somatogenic receptor ligand. 125I-bovine growth hormone was cross-linked to a Golgi/endosome fraction isolated from livers of pregnant rats followed by sodium dodecylsulfate-polyacrylamide gel electrophoresis under reducing conditions. Three major labeled bands of molecular weights, Mr 95,000, 86,000 and 43,000 and one minor labeled band of Mr 55,000 were seen after correction for bound ligand assuming a 1:1 binding ratio of the ligand. The 95,000 molecular weight species was found to be Triton X-100 insoluble. Sedimentation coefficient, partial specific volume and Stokes radius were determined to 5.0 0.72 ml/g and 61 Å, respectively for the 125I-bovine growth hormone-receptor-Triton X-100 complex. Based on these figures, the molecular weight of the receptor-Triton X-100 complex was calculated to 109,100. The 43,000 labeled band found in Golgi/endosomes was found to be a glycoprotein with a N-linked sugar residue. In residual microsomes a labeled band of Mr 33,000 seems to contain a high mannose structure." @default.
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- W21000857 date "1987-01-01" @default.
- W21000857 modified "2023-09-30" @default.
- W21000857 title "CHARACTERIZATION OF THE SOMATOGENIC RECEPTOR IN RAT LIVER" @default.
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- W21000857 doi "https://doi.org/10.1016/b978-0-08-035588-7.50063-7" @default.
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