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• W2100088958 abstract "Abstract As alternative microbial hosts for butanol production with organic-solvent tolerant trait are in high demands, a butanol-tolerant bacterium, Bacillus subtilis GRSW2-B1, was thus isolated. Its tolerance covered a range of organic solvents at high concentration (5%v/v), with remarkable tolerance in particular to butanol and alcohol groups. It was susceptible for butanol acclimatization, which resulted in significant tolerance improvement. It has versatility for application in a variety of fermentation process because it has superior tolerance when cells were exposed to butanol either as high-density, late-exponential grown cells (up to 5%v/v) or under growing conditions (up to 2.25%v/v). Genetic transformation procedure was optimized, yielding the highest efficiency at 5.17 × 10 3 colony forming unit (μg DNA) -1 . Gene expression could be effectively driven by several promoters with different levels, where as the highest expression was observed with a xylose promoter. The constructed vector was stably maintained in the transformants, in the presence or absence of butanol stress. Adverse effect of efflux-mediated tetracycline resistance determinant (TetL) to bacterial organic-solvent tolerance property was unexpectedly observed and thus discussed. Overall results indicate that B. subtilis GRSW2-B1 has potential to be engineered and further established as a genetic host for bioproduction of butanol." @default.
• W2100088958 created "2016-06-24" @default.
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• W2100088958 date "2011-05-30" @default.
• W2100088958 modified "2023-10-18" @default.
• W2100088958 title "Development of butanol-tolerant Bacillus subtilis strain GRSW2-B1 as a potential bioproduction host" @default.
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• W2100088958 doi "https://doi.org/10.1186/2191-0855-1-10" @default.
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