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• W2100091869 abstract "Abstract Multiple mechanisms have been proposed for gene silencing in Saccharomyces cerevisiae, ranging from steric occlusion of DNA binding proteins from their recognition sequences in silenced chromatin to a specific block in the formation of the preinitiation complex to a block in transcriptional elongation. This study provided strong support for the steric occlusion mechanism by the discovery that RNA polymerase of bacteriophage T7 could be substantially blocked from transcribing from its cognate promoter when embedded in silenced chromatin. Moreover, unlike previous suggestions, we found no evidence for stalled RNA polymerase II within silenced chromatin. The effectiveness of the Sir protein–based silencing mechanism to block transcription activated by Gal4 at promoters in the domain of silenced chromatin was marginal, yet it improved when tested against mutant forms of the Gal4 protein, highlighting a role for specific activators in their sensitivity to gene silencing." @default.
• W2100091869 created "2016-06-24" @default.
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• W2100091869 creator A5066113975 @default.
• W2100091869 date "2015-08-01" @default.
• W2100091869 modified "2023-10-17" @default.
• W2100091869 title "On the Mechanism of Gene Silencing in <i>Saccharomyces cerevisiae</i>" @default.
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• W2100091869 doi "https://doi.org/10.1534/g3.115.018515" @default.
• W2100091869 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4528331" @default.
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