Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100092278> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2100092278 endingPage "496" @default.
- W2100092278 startingPage "491" @default.
- W2100092278 abstract "We have identified a P450(cam) mutation, L244A, that mitigates the affinity for imidazole and substituted imidazoles while maintaining a high affinity for the natural substrate camphor. The P450(cam) L244A crystal structure solved in the absence of any ligand reveals that the I-helix is displaced inwards by over 1 A in response to the cavity created by the change from leucine to alanine. Furthermore, the crystal structures of imidazole-bound P450(cam) and the 1-methylimidazole-bound P450(cam) L244A mutant reveal that the ligands have distinct binding modes in the two proteins. Whereas in wild-type P450(cam) the imidazole coordinates to the iron in an orientation roughly perpendicular to the plane of the heme, in the L244A mutant the rearranged I helix, and specifically residue Val247, forces the imidazole into an orientation almost parallel to the heme that impairs its ability to coordinate to the heme iron. As a result, the imidazole is much more weakly bound to the mutant than it is to the wild-type enzyme. Despite the constriction of the active site by the mutation, previous work with the L244A mutant has shown that it oxidizes larger substrates than the wild-type enzyme. This paradoxical situation, in which a mutation that nominally increases the active site cavity appears to decrease it, suggests that the mutation actually increases the active site maleability, allowing it to better expand to oxidize larger substrates." @default.
- W2100092278 created "2016-06-24" @default.
- W2100092278 creator A5003621282 @default.
- W2100092278 creator A5021356066 @default.
- W2100092278 creator A5084725183 @default.
- W2100092278 date "2006-09-02" @default.
- W2100092278 modified "2023-09-23" @default.
- W2100092278 title "Cytochrome P450 active site plasticity: attenuation of imidazole binding in cytochrome P450cam by an L244A mutation" @default.
- W2100092278 cites W1504969732 @default.
- W2100092278 cites W1978655692 @default.
- W2100092278 cites W2060107894 @default.
- W2100092278 cites W2133932584 @default.
- W2100092278 cites W2165811356 @default.
- W2100092278 doi "https://doi.org/10.1093/protein/gzl035" @default.
- W2100092278 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16943206" @default.
- W2100092278 hasPublicationYear "2006" @default.
- W2100092278 type Work @default.
- W2100092278 sameAs 2100092278 @default.
- W2100092278 citedByCount "22" @default.
- W2100092278 countsByYear W21000922782012 @default.
- W2100092278 countsByYear W21000922782013 @default.
- W2100092278 countsByYear W21000922782016 @default.
- W2100092278 countsByYear W21000922782019 @default.
- W2100092278 countsByYear W21000922782020 @default.
- W2100092278 countsByYear W21000922782021 @default.
- W2100092278 crossrefType "journal-article" @default.
- W2100092278 hasAuthorship W2100092278A5003621282 @default.
- W2100092278 hasAuthorship W2100092278A5021356066 @default.
- W2100092278 hasAuthorship W2100092278A5084725183 @default.
- W2100092278 hasBestOaLocation W21000922782 @default.
- W2100092278 hasConcept C104317684 @default.
- W2100092278 hasConcept C107824862 @default.
- W2100092278 hasConcept C143065580 @default.
- W2100092278 hasConcept C181199279 @default.
- W2100092278 hasConcept C185592680 @default.
- W2100092278 hasConcept C207583985 @default.
- W2100092278 hasConcept C2776217839 @default.
- W2100092278 hasConcept C2780768313 @default.
- W2100092278 hasConcept C2780874372 @default.
- W2100092278 hasConcept C41183919 @default.
- W2100092278 hasConcept C55493867 @default.
- W2100092278 hasConcept C71240020 @default.
- W2100092278 hasConcept C8010536 @default.
- W2100092278 hasConceptScore W2100092278C104317684 @default.
- W2100092278 hasConceptScore W2100092278C107824862 @default.
- W2100092278 hasConceptScore W2100092278C143065580 @default.
- W2100092278 hasConceptScore W2100092278C181199279 @default.
- W2100092278 hasConceptScore W2100092278C185592680 @default.
- W2100092278 hasConceptScore W2100092278C207583985 @default.
- W2100092278 hasConceptScore W2100092278C2776217839 @default.
- W2100092278 hasConceptScore W2100092278C2780768313 @default.
- W2100092278 hasConceptScore W2100092278C2780874372 @default.
- W2100092278 hasConceptScore W2100092278C41183919 @default.
- W2100092278 hasConceptScore W2100092278C55493867 @default.
- W2100092278 hasConceptScore W2100092278C71240020 @default.
- W2100092278 hasConceptScore W2100092278C8010536 @default.
- W2100092278 hasIssue "11" @default.
- W2100092278 hasLocation W21000922781 @default.
- W2100092278 hasLocation W21000922782 @default.
- W2100092278 hasLocation W21000922783 @default.
- W2100092278 hasOpenAccess W2100092278 @default.
- W2100092278 hasPrimaryLocation W21000922781 @default.
- W2100092278 hasRelatedWork W1894529473 @default.
- W2100092278 hasRelatedWork W1987491348 @default.
- W2100092278 hasRelatedWork W2000796455 @default.
- W2100092278 hasRelatedWork W2073369674 @default.
- W2100092278 hasRelatedWork W2088530786 @default.
- W2100092278 hasRelatedWork W2100092278 @default.
- W2100092278 hasRelatedWork W2286939631 @default.
- W2100092278 hasRelatedWork W258580054 @default.
- W2100092278 hasRelatedWork W2808318619 @default.
- W2100092278 hasRelatedWork W214510971 @default.
- W2100092278 hasVolume "19" @default.
- W2100092278 isParatext "false" @default.
- W2100092278 isRetracted "false" @default.
- W2100092278 magId "2100092278" @default.
- W2100092278 workType "article" @default.