FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100128246> ?p ?o ?g. }

• W2100128246 endingPage "52" @default.
• W2100128246 startingPage "4444" @default.
• W2100128246 abstract "A monoclonal antibody (7B10) which displays differential reactivity with breast carcinomas compared to benign lesions or normal breast tissue was selected by fusion of spleen cells from BALB/c mice immunized with the T47D human mammary carcinoma cell line. The antigen, recognized by 7B10 on T47D cells, appeared to be both surface and cytoplasm localized, as demonstrated by indirect immunofluorescence, immunoperoxidase, and electron microscopy studies. This antibody (IgG1) bound with four human breast cancer cell lines (T47D, MCF7, ZR-75-1, and HSL53) which express estrogen receptors. No binding was observed with cancer cell lines of other origin or with normal cells. In vivo, by immunoperoxidase staining of frozen sections of normal breast, the antigen recognized by 7B10 appeared to be located on epithelial cell membranes, whereas in benign and malignant mammary disorders, staining also involved the cytoplasm, as confirmed by electron microscopy on fresh cancer tissue. On formalin-fixed, paraffin-embedded sections, cytoplasmic staining was detected in breast cancer, but no immunostaining was observed with benign lesions or normal breast. In paraffin sections, most normal tissues investigated did not react with 7B10 antibody. However, ducts in the parotid gland, tubules in the kidney and some biliary ducts, and apocrine glands in the skin showed irregular, diffuse weak staining. 7B10 was unreactive with adenocarcinomas of origin other than breast, except for some cells in ovarian clear cell carcinoma. No reactivity was observed with squamous carcinomas, lymphomas, or melanomas. The antigen recognized by 7B10 appeared to be a Mr 32,000 protein, as identified by immunoprecipitation from extracts of T47D after labeling with [35S]methionine. Since the antigen was present only on the membrane of differentiated normal mammary epithelial cells, and was also expressed in the cytoplasm of tumor cells, it may be of interest in immunological studies of mammary epithelial cell differentiation. Moreover, since in formalin-fixed tissues immunostaining is virtually confined to mammary carcinomas, monoclonal antibody 7B10 may have diagnostic applications in breast cancer." @default.
• W2100128246 created "2016-06-24" @default.
• W2100128246 creator A5004905312 @default.
• W2100128246 creator A5014689553 @default.
• W2100128246 creator A5041015671 @default.
• W2100128246 creator A5071065958 @default.
• W2100128246 creator A5075237282 @default.
• W2100128246 date "1987-08-15" @default.
• W2100128246 modified "2023-09-23" @default.
• W2100128246 title "A novel monoclonal antibody (7B10) with differential reactivity between human mammary carcinoma and normal breast." @default.
• W2100128246 cites W1493303652 @default.
• W2100128246 cites W1573948298 @default.
• W2100128246 cites W1605862162 @default.
• W2100128246 cites W1974281619 @default.
• W2100128246 cites W2001269969 @default.
• W2100128246 cites W2017987878 @default.
• W2100128246 cites W2021287098 @default.
• W2100128246 cites W2023761151 @default.
• W2100128246 cites W2031289000 @default.
• W2100128246 cites W2031928907 @default.
• W2100128246 cites W2053503919 @default.
• W2100128246 cites W2061702003 @default.
• W2100128246 cites W2067935330 @default.
• W2100128246 cites W2073131935 @default.
• W2100128246 cites W2084168547 @default.
• W2100128246 cites W2084487251 @default.
• W2100128246 cites W2091850539 @default.
• W2100128246 cites W2103262956 @default.
• W2100128246 cites W2110872750 @default.
• W2100128246 cites W2125880960 @default.
• W2100128246 cites W2128635872 @default.
• W2100128246 cites W2133330674 @default.
• W2100128246 cites W2138101780 @default.
• W2100128246 cites W2144626266 @default.
• W2100128246 cites W2154370298 @default.
• W2100128246 cites W2154917910 @default.
• W2100128246 cites W2159973775 @default.
• W2100128246 cites W2267494386 @default.
• W2100128246 cites W2318566650 @default.
• W2100128246 cites W2398443726 @default.
• W2100128246 cites W2409143970 @default.
• W2100128246 cites W2417540681 @default.
• W2100128246 cites W3145716590 @default.
• W2100128246 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3607774" @default.
• W2100128246 hasPublicationYear "1987" @default.
• W2100128246 type Work @default.
• W2100128246 sameAs 2100128246 @default.
• W2100128246 citedByCount "5" @default.
• W2100128246 crossrefType "journal-article" @default.
• W2100128246 hasAuthorship W2100128246A5004905312 @default.
• W2100128246 hasAuthorship W2100128246A5014689553 @default.
• W2100128246 hasAuthorship W2100128246A5041015671 @default.
• W2100128246 hasAuthorship W2100128246A5071065958 @default.
• W2100128246 hasAuthorship W2100128246A5075237282 @default.
• W2100128246 hasConcept C121608353 @default.
• W2100128246 hasConcept C128102431 @default.
• W2100128246 hasConcept C142724271 @default.
• W2100128246 hasConcept C147483822 @default.
• W2100128246 hasConcept C159654299 @default.
• W2100128246 hasConcept C203014093 @default.
• W2100128246 hasConcept C204232928 @default.
• W2100128246 hasConcept C2781182431 @default.
• W2100128246 hasConcept C4224716 @default.
• W2100128246 hasConcept C542903549 @default.
• W2100128246 hasConcept C54355233 @default.
• W2100128246 hasConcept C71924100 @default.
• W2100128246 hasConcept C74864618 @default.
• W2100128246 hasConcept C86803240 @default.
• W2100128246 hasConceptScore W2100128246C121608353 @default.
• W2100128246 hasConceptScore W2100128246C128102431 @default.
• W2100128246 hasConceptScore W2100128246C142724271 @default.
• W2100128246 hasConceptScore W2100128246C147483822 @default.
• W2100128246 hasConceptScore W2100128246C159654299 @default.
• W2100128246 hasConceptScore W2100128246C203014093 @default.
• W2100128246 hasConceptScore W2100128246C204232928 @default.
• W2100128246 hasConceptScore W2100128246C2781182431 @default.
• W2100128246 hasConceptScore W2100128246C4224716 @default.
• W2100128246 hasConceptScore W2100128246C542903549 @default.
• W2100128246 hasConceptScore W2100128246C54355233 @default.
• W2100128246 hasConceptScore W2100128246C71924100 @default.
• W2100128246 hasConceptScore W2100128246C74864618 @default.
• W2100128246 hasConceptScore W2100128246C86803240 @default.
• W2100128246 hasIssue "16" @default.
• W2100128246 hasLocation W21001282461 @default.
• W2100128246 hasOpenAccess W2100128246 @default.
• W2100128246 hasPrimaryLocation W21001282461 @default.
• W2100128246 hasRelatedWork W1978777644 @default.
• W2100128246 hasRelatedWork W1983672032 @default.
• W2100128246 hasRelatedWork W2011115754 @default.
• W2100128246 hasRelatedWork W2026195091 @default.
• W2100128246 hasRelatedWork W2052178042 @default.
• W2100128246 hasRelatedWork W2053610471 @default.
• W2100128246 hasRelatedWork W2143911359 @default.
• W2100128246 hasRelatedWork W2168239348 @default.
• W2100128246 hasRelatedWork W2997885895 @default.
• W2100128246 hasRelatedWork W132482127 @default.
• W2100128246 hasVolume "47" @default.
• W2100128246 isParatext "false" @default.

• next