FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100130131> ?p ?o ?g. }

• W2100130131 endingPage "1282" @default.
• W2100130131 startingPage "1275" @default.
• W2100130131 abstract "Purpose The aim of the study was to determine the in vitro effects of plasma-mediated bipolar radiofrequency ablation on human chondrocyte compensatory proliferation and inflammatory mediator expression. Methods Human articular cartilage biopsy specimens, from total knee replacement, and human chondrocytes in alginate culture, from patients undergoing autologous chondrocyte implantation, were exposed to plasma ablation with a Paragon T2 probe (ArthroCare, Austin, TX). Instantaneous chondrocyte death was investigated with live/dead assays of biopsy specimens and cell cultures. Chondrocyte proliferation was determined by Hoechst staining of DNA on days 3 and 6. Messenger RNA expression of IL-1β, IL-6, IL-8, tumor necrosis factor α, high-mobility group protein B1, matrix metalloproteinase 13, type IIA collagen, and versican was determined on days 3 and 6. Results Live/dead imaging showed a well-defined local margin of cell death ranging from 150 to 200 μm deep, both in the alginate gel and in the biopsy specimens exposed to plasma ablation. The ablation-exposed group showed a significant proliferation increase compared with control on day 3 (P < .043). There were significant increases compared with control in IL-6 expression on day 3 (P < .020) and day 6 (P < .045) and in IL-8 expression on day 3 (P < .048). No differences were seen for IL-1β, tumor necrosis factor α, high-mobility group protein B1, matrix metalloproteinase 13, type II collagen, or versican. Conclusions This study has shown that exposure to plasma-mediated ablation induces a well-defined area of immediate cell death and a short-term increase in proliferation with human articular chondrocytes in vitro. The exposure also alters cytokine expression for the same period, causing upregulation of IL-6 and IL-8. Clinical Relevance The results show the potential of plasma-mediated ablation to cause the onset of a tissue regeneration response with human articular cartilage. The aim of the study was to determine the in vitro effects of plasma-mediated bipolar radiofrequency ablation on human chondrocyte compensatory proliferation and inflammatory mediator expression. Human articular cartilage biopsy specimens, from total knee replacement, and human chondrocytes in alginate culture, from patients undergoing autologous chondrocyte implantation, were exposed to plasma ablation with a Paragon T2 probe (ArthroCare, Austin, TX). Instantaneous chondrocyte death was investigated with live/dead assays of biopsy specimens and cell cultures. Chondrocyte proliferation was determined by Hoechst staining of DNA on days 3 and 6. Messenger RNA expression of IL-1β, IL-6, IL-8, tumor necrosis factor α, high-mobility group protein B1, matrix metalloproteinase 13, type IIA collagen, and versican was determined on days 3 and 6. Live/dead imaging showed a well-defined local margin of cell death ranging from 150 to 200 μm deep, both in the alginate gel and in the biopsy specimens exposed to plasma ablation. The ablation-exposed group showed a significant proliferation increase compared with control on day 3 (P < .043). There were significant increases compared with control in IL-6 expression on day 3 (P < .020) and day 6 (P < .045) and in IL-8 expression on day 3 (P < .048). No differences were seen for IL-1β, tumor necrosis factor α, high-mobility group protein B1, matrix metalloproteinase 13, type II collagen, or versican. This study has shown that exposure to plasma-mediated ablation induces a well-defined area of immediate cell death and a short-term increase in proliferation with human articular chondrocytes in vitro. The exposure also alters cytokine expression for the same period, causing upregulation of IL-6 and IL-8." @default.
• W2100130131 created "2016-06-24" @default.
• W2100130131 creator A5030504802 @default.
• W2100130131 creator A5038842105 @default.
• W2100130131 creator A5056288205 @default.
• W2100130131 creator A5088279030 @default.
• W2100130131 date "2012-09-01" @default.
• W2100130131 modified "2023-10-18" @default.
• W2100130131 title "Bipolar Radiofrequency Plasma Ablation Induces Proliferation and Alters Cytokine Expression in Human Articular Cartilage Chondrocytes" @default.
• W2100130131 cites W1408570629 @default.
• W2100130131 cites W1655390247 @default.
• W2100130131 cites W192836234 @default.
• W2100130131 cites W1955143826 @default.
• W2100130131 cites W1963315781 @default.
• W2100130131 cites W1970072272 @default.
• W2100130131 cites W1988517878 @default.
• W2100130131 cites W1989271162 @default.
• W2100130131 cites W1992481999 @default.
• W2100130131 cites W1994261002 @default.
• W2100130131 cites W1994713541 @default.
• W2100130131 cites W2014545259 @default.
• W2100130131 cites W2018397726 @default.
• W2100130131 cites W2019055276 @default.
• W2100130131 cites W2030431377 @default.
• W2100130131 cites W2041201304 @default.
• W2100130131 cites W2049400205 @default.
• W2100130131 cites W2052020977 @default.
• W2100130131 cites W2053342448 @default.
• W2100130131 cites W2063990158 @default.
• W2100130131 cites W2076348894 @default.
• W2100130131 cites W2088564623 @default.
• W2100130131 cites W2107055346 @default.
• W2100130131 cites W2116548852 @default.
• W2100130131 cites W2119171662 @default.
• W2100130131 cites W2122627288 @default.
• W2100130131 cites W2125105098 @default.
• W2100130131 cites W2139170681 @default.
• W2100130131 cites W2139924646 @default.
• W2100130131 cites W2144814682 @default.
• W2100130131 cites W2149459392 @default.
• W2100130131 cites W2161277214 @default.
• W2100130131 cites W2163440809 @default.
• W2100130131 cites W2166892087 @default.
• W2100130131 cites W2171760060 @default.
• W2100130131 cites W4232900352 @default.
• W2100130131 doi "https://doi.org/10.1016/j.arthro.2012.01.005" @default.
• W2100130131 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22480788" @default.
• W2100130131 hasPublicationYear "2012" @default.
• W2100130131 type Work @default.
• W2100130131 sameAs 2100130131 @default.
• W2100130131 citedByCount "11" @default.
• W2100130131 countsByYear W21001301312015 @default.
• W2100130131 countsByYear W21001301312016 @default.
• W2100130131 countsByYear W21001301312017 @default.
• W2100130131 countsByYear W21001301312019 @default.
• W2100130131 countsByYear W21001301312020 @default.
• W2100130131 countsByYear W21001301312022 @default.
• W2100130131 countsByYear W21001301312023 @default.
• W2100130131 crossrefType "journal-article" @default.
• W2100130131 hasAuthorship W2100130131A5030504802 @default.
• W2100130131 hasAuthorship W2100130131A5038842105 @default.
• W2100130131 hasAuthorship W2100130131A5056288205 @default.
• W2100130131 hasAuthorship W2100130131A5088279030 @default.
• W2100130131 hasConcept C105702510 @default.
• W2100130131 hasConcept C142724271 @default.
• W2100130131 hasConcept C16685009 @default.
• W2100130131 hasConcept C16930146 @default.
• W2100130131 hasConcept C17991360 @default.
• W2100130131 hasConcept C185592680 @default.
• W2100130131 hasConcept C203014093 @default.
• W2100130131 hasConcept C2778690821 @default.
• W2100130131 hasConcept C2780550940 @default.
• W2100130131 hasConcept C2781013607 @default.
• W2100130131 hasConcept C2781403057 @default.
• W2100130131 hasConcept C71924100 @default.
• W2100130131 hasConceptScore W2100130131C105702510 @default.
• W2100130131 hasConceptScore W2100130131C142724271 @default.
• W2100130131 hasConceptScore W2100130131C16685009 @default.
• W2100130131 hasConceptScore W2100130131C16930146 @default.
• W2100130131 hasConceptScore W2100130131C17991360 @default.
• W2100130131 hasConceptScore W2100130131C185592680 @default.
• W2100130131 hasConceptScore W2100130131C203014093 @default.
• W2100130131 hasConceptScore W2100130131C2778690821 @default.
• W2100130131 hasConceptScore W2100130131C2780550940 @default.
• W2100130131 hasConceptScore W2100130131C2781013607 @default.
• W2100130131 hasConceptScore W2100130131C2781403057 @default.
• W2100130131 hasConceptScore W2100130131C71924100 @default.
• W2100130131 hasIssue "9" @default.
• W2100130131 hasLocation W21001301311 @default.
• W2100130131 hasLocation W21001301312 @default.
• W2100130131 hasOpenAccess W2100130131 @default.
• W2100130131 hasPrimaryLocation W21001301311 @default.
• W2100130131 hasRelatedWork W1480797633 @default.
• W2100130131 hasRelatedWork W1965971430 @default.
• W2100130131 hasRelatedWork W2039195542 @default.
• W2100130131 hasRelatedWork W2490525963 @default.
• W2100130131 hasRelatedWork W3113789775 @default.
• W2100130131 hasRelatedWork W3214112236 @default.

• next