FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100147400> ?p ?o ?g. }

• W2100147400 endingPage "2762" @default.
• W2100147400 startingPage "2754" @default.
• W2100147400 abstract "Abstract Protein citrullination originates from enzymatic deimination of polypeptide‐bound arginine and is involved in various biological processes during health and disease. However, tools required for a detailed and targeted proteomic analysis of citrullinated proteins in situ , including their citrullination sites, are limited. A widely used technique for detection of citrullinated proteins relies on antibody staining after specific derivatization of citrulline residues by 2,3‐butanedione and antipyrine. We have recently reported on the details of this reaction. Here, we show that this chemical modification can be utilized to specifically detect and identify citrullinated peptides and their citrullination sites by liquid chromatography/tandem mass spectrometry (LC/MS/MS) analysis. Using model compounds, we demonstrate that in collision‐induced dissociation (CID) a specific, modification‐derived fragment ion appears as the dominating signal at m/z 201.1 in the MS/MS spectra. When applying electron transfer dissociation (ETD), however, the chemical modification of citrulline remained intact and extensive sequence coverage allowed identification of peptides and their citrullination sites. Therefore, LC/MS/MS analysis with alternating CID and ETD has been performed, using CID for specific, signature ion‐based detection of derivatized citrullinated peptides and ETD for sequence determination. The usefulness of this targeted analysis was demonstrated by identifying citrullination sites in myelin basic protein deiminated in vitro . Combining antibody‐based enrichment of chemically modified citrulline‐containing peptides with specific mass spectrometric detection will increase the potential of such a targeted analysis of protein citrullination in the future. Copyright © 2009 John Wiley & Sons, Ltd." @default.
• W2100147400 created "2016-06-24" @default.
• W2100147400 creator A5025244372 @default.
• W2100147400 creator A5053135148 @default.
• W2100147400 creator A5065290460 @default.
• W2100147400 creator A5086049465 @default.
• W2100147400 date "2009-07-28" @default.
• W2100147400 modified "2023-10-14" @default.
• W2100147400 title "Targeted analysis of protein citrullination using chemical modification and tandem mass spectrometry" @default.
• W2100147400 cites W1582204239 @default.
• W2100147400 cites W1586192659 @default.
• W2100147400 cites W1969515398 @default.
• W2100147400 cites W1971472666 @default.
• W2100147400 cites W1985596848 @default.
• W2100147400 cites W1991787417 @default.
• W2100147400 cites W1995734706 @default.
• W2100147400 cites W1996985500 @default.
• W2100147400 cites W1997785774 @default.
• W2100147400 cites W2002274092 @default.
• W2100147400 cites W2002832567 @default.
• W2100147400 cites W2005699747 @default.
• W2100147400 cites W2012207611 @default.
• W2100147400 cites W2013573826 @default.
• W2100147400 cites W2017792299 @default.
• W2100147400 cites W2024603281 @default.
• W2100147400 cites W2027102068 @default.
• W2100147400 cites W2033199777 @default.
• W2100147400 cites W2033889320 @default.
• W2100147400 cites W2037205941 @default.
• W2100147400 cites W2039461477 @default.
• W2100147400 cites W2047308059 @default.
• W2100147400 cites W2050314725 @default.
• W2100147400 cites W2057896776 @default.
• W2100147400 cites W2071700927 @default.
• W2100147400 cites W2075045273 @default.
• W2100147400 cites W2079056345 @default.
• W2100147400 cites W2083656801 @default.
• W2100147400 cites W2086420062 @default.
• W2100147400 cites W2092813655 @default.
• W2100147400 cites W2093483361 @default.
• W2100147400 cites W2099255819 @default.
• W2100147400 cites W2100637259 @default.
• W2100147400 cites W2107769121 @default.
• W2100147400 cites W2116265555 @default.
• W2100147400 cites W2123805036 @default.
• W2100147400 cites W2123960992 @default.
• W2100147400 cites W2128987420 @default.
• W2100147400 cites W2136131602 @default.
• W2100147400 cites W2140407684 @default.
• W2100147400 cites W2141037299 @default.
• W2100147400 cites W2161083956 @default.
• W2100147400 cites W2164744416 @default.
• W2100147400 doi "https://doi.org/10.1002/rcm.4185" @default.
• W2100147400 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19639564" @default.
• W2100147400 hasPublicationYear "2009" @default.
• W2100147400 type Work @default.
• W2100147400 sameAs 2100147400 @default.
• W2100147400 citedByCount "40" @default.
• W2100147400 countsByYear W21001474002012 @default.
• W2100147400 countsByYear W21001474002013 @default.
• W2100147400 countsByYear W21001474002014 @default.
• W2100147400 countsByYear W21001474002016 @default.
• W2100147400 countsByYear W21001474002017 @default.
• W2100147400 countsByYear W21001474002018 @default.
• W2100147400 countsByYear W21001474002019 @default.
• W2100147400 countsByYear W21001474002020 @default.
• W2100147400 countsByYear W21001474002021 @default.
• W2100147400 countsByYear W21001474002022 @default.
• W2100147400 countsByYear W21001474002023 @default.
• W2100147400 crossrefType "journal-article" @default.
• W2100147400 hasAuthorship W2100147400A5025244372 @default.
• W2100147400 hasAuthorship W2100147400A5053135148 @default.
• W2100147400 hasAuthorship W2100147400A5065290460 @default.
• W2100147400 hasAuthorship W2100147400A5086049465 @default.
• W2100147400 hasConcept C104317684 @default.
• W2100147400 hasConcept C162356407 @default.
• W2100147400 hasConcept C185592680 @default.
• W2100147400 hasConcept C2776923573 @default.
• W2100147400 hasConcept C2777109674 @default.
• W2100147400 hasConcept C2777468819 @default.
• W2100147400 hasConcept C2779129087 @default.
• W2100147400 hasConcept C31827203 @default.
• W2100147400 hasConcept C43617362 @default.
• W2100147400 hasConcept C46111723 @default.
• W2100147400 hasConcept C48632299 @default.
• W2100147400 hasConcept C515207424 @default.
• W2100147400 hasConcept C55493867 @default.
• W2100147400 hasConceptScore W2100147400C104317684 @default.
• W2100147400 hasConceptScore W2100147400C162356407 @default.
• W2100147400 hasConceptScore W2100147400C185592680 @default.
• W2100147400 hasConceptScore W2100147400C2776923573 @default.
• W2100147400 hasConceptScore W2100147400C2777109674 @default.
• W2100147400 hasConceptScore W2100147400C2777468819 @default.
• W2100147400 hasConceptScore W2100147400C2779129087 @default.
• W2100147400 hasConceptScore W2100147400C31827203 @default.
• W2100147400 hasConceptScore W2100147400C43617362 @default.
• W2100147400 hasConceptScore W2100147400C46111723 @default.
• W2100147400 hasConceptScore W2100147400C48632299 @default.

• next