FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100160494> ?p ?o ?g. }

• W2100160494 endingPage "121" @default.
• W2100160494 startingPage "110" @default.
• W2100160494 abstract "The first committed step of the biosynthetic pathway leading to uridine-5′-diphospho-N-acetyl-D-glucosamine (UDP-GlcNAc) is catalyzed by glucosamine-6-phosphate synthase (GlcN-6-P synthase), an enzyme proposed as a potential antifungal chemotherapy target. Here, we describe the purification and biochemical characterization of the native enzyme from the dimorphic pathogenic fungus Sporothrix schenckii. The availability of the pure protein facilitated its biochemical characterization. The enzyme exhibited subunit and native molecular masses of 79 and 350±5 kDa, respectively, suggesting a homotetrameric structure. Isoelectric point was 6.26 and Km values for fructose-6-phosphate and L-glutamine were 1.12±0.3 and 2.2±0.7 mM, respectively. Inhibition of activity by UDP-GlcNAc was enhanced by Glc-6-P and phosphorylation stimulated GlcN-6-P synthase activity without affecting the enzyme sensitivity to the aminosugar. A glutamine analogue, FMDP [N3-(4-methoxyfumaroyl)-L-2,3-diaminopropanoic acid] was a more potent inhibitor of activity than ADMP (2-Amino-2-deoxy-D-mannitol-6-phosphate) but the latter was a stronger inhibitor of growth in two culture media. To our knowledge, this is the first report on the purification and biochemical characterization of a non-recombinant GlcN-6-P synthase from a true dimorphic fungus. Inhibition of enzyme activity and fungal growth by specific inhibitors of GlcN-6-P synthase strongly reinforces the role of this enzyme as a potential target for antifungal chemotherapy." @default.
• W2100160494 created "2016-06-24" @default.
• W2100160494 creator A5014266318 @default.
• W2100160494 creator A5024091456 @default.
• W2100160494 creator A5033303668 @default.
• W2100160494 creator A5047939604 @default.
• W2100160494 creator A5058921472 @default.
• W2100160494 date "2010-02-01" @default.
• W2100160494 modified "2023-09-23" @default.
• W2100160494 title "Sporothrix schenckii: purification and partial biochemical characterization of glucosamine-6-phosphate synthase, a potential antifungal target" @default.
• W2100160494 cites W1493509066 @default.
• W2100160494 cites W1516265214 @default.
• W2100160494 cites W1553140039 @default.
• W2100160494 cites W1570494654 @default.
• W2100160494 cites W1589370173 @default.
• W2100160494 cites W1620822945 @default.
• W2100160494 cites W1787326210 @default.
• W2100160494 cites W1887989586 @default.
• W2100160494 cites W1963594570 @default.
• W2100160494 cites W1968583684 @default.
• W2100160494 cites W1970003648 @default.
• W2100160494 cites W1978369141 @default.
• W2100160494 cites W1980659401 @default.
• W2100160494 cites W1982345335 @default.
• W2100160494 cites W1988731539 @default.
• W2100160494 cites W1992167837 @default.
• W2100160494 cites W1993109819 @default.
• W2100160494 cites W1993178150 @default.
• W2100160494 cites W1998546461 @default.
• W2100160494 cites W1999304465 @default.
• W2100160494 cites W2000717525 @default.
• W2100160494 cites W2000957418 @default.
• W2100160494 cites W2011405433 @default.
• W2100160494 cites W2015777145 @default.
• W2100160494 cites W2015822275 @default.
• W2100160494 cites W2018579450 @default.
• W2100160494 cites W2018698731 @default.
• W2100160494 cites W2021123597 @default.
• W2100160494 cites W2029812798 @default.
• W2100160494 cites W2030698809 @default.
• W2100160494 cites W2034069593 @default.
• W2100160494 cites W2044223767 @default.
• W2100160494 cites W2047823732 @default.
• W2100160494 cites W2052903221 @default.
• W2100160494 cites W2054154819 @default.
• W2100160494 cites W2055268632 @default.
• W2100160494 cites W2055318090 @default.
• W2100160494 cites W2055823541 @default.
• W2100160494 cites W2078059986 @default.
• W2100160494 cites W2084274544 @default.
• W2100160494 cites W2093236981 @default.
• W2100160494 cites W2108427758 @default.
• W2100160494 cites W2126082806 @default.
• W2100160494 cites W2129364654 @default.
• W2100160494 cites W2129646449 @default.
• W2100160494 cites W2155036659 @default.
• W2100160494 cites W2163116037 @default.
• W2100160494 cites W2171799925 @default.
• W2100160494 cites W2338840804 @default.
• W2100160494 cites W2343267183 @default.
• W2100160494 cites W2395981834 @default.
• W2100160494 cites W2550531492 @default.
• W2100160494 cites W3118834441 @default.
• W2100160494 cites W4233985752 @default.
• W2100160494 cites W4249684293 @default.
• W2100160494 cites W4293247451 @default.
• W2100160494 cites W87658133 @default.
• W2100160494 doi "https://doi.org/10.3109/13693780902856030" @default.
• W2100160494 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19353425" @default.
• W2100160494 hasPublicationYear "2010" @default.
• W2100160494 type Work @default.
• W2100160494 sameAs 2100160494 @default.
• W2100160494 citedByCount "11" @default.
• W2100160494 countsByYear W21001604942012 @default.
• W2100160494 countsByYear W21001604942013 @default.
• W2100160494 countsByYear W21001604942014 @default.
• W2100160494 countsByYear W21001604942015 @default.
• W2100160494 countsByYear W21001604942018 @default.
• W2100160494 countsByYear W21001604942021 @default.
• W2100160494 countsByYear W21001604942022 @default.
• W2100160494 crossrefType "journal-article" @default.
• W2100160494 hasAuthorship W2100160494A5014266318 @default.
• W2100160494 hasAuthorship W2100160494A5024091456 @default.
• W2100160494 hasAuthorship W2100160494A5033303668 @default.
• W2100160494 hasAuthorship W2100160494A5047939604 @default.
• W2100160494 hasAuthorship W2100160494A5058921472 @default.
• W2100160494 hasBestOaLocation W21001604941 @default.
• W2100160494 hasConcept C112243037 @default.
• W2100160494 hasConcept C153911025 @default.
• W2100160494 hasConcept C181199279 @default.
• W2100160494 hasConcept C203014093 @default.
• W2100160494 hasConcept C2776872704 @default.
• W2100160494 hasConcept C2777250718 @default.
• W2100160494 hasConcept C2778125326 @default.
• W2100160494 hasConcept C2779349466 @default.
• W2100160494 hasConcept C28212737 @default.
• W2100160494 hasConcept C2909991210 @default.
• W2100160494 hasConcept C515207424 @default.

• next