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• W2100179968 abstract "The U2 snRNP binds to the site of branch formation during splicing of mammalian pre-mRNA in vitro. In Saccharomyces cerevisiae the branch site is within the so-called TACTAAC box (UACUAAC box), an absolutely conserved intron sequence required for splicing. Based on the identification and sequence of a U2 analogue in yeast, a specific base pairing interaction between the UACUAAC box and a highly conserved region of this snRNA can be proposed. To test this hypothesis, we have taken advantage of two mutations constructed previously in the UACUAAC box of an actin-HIS4 fusion. These mutant strains were transformed with stable plasmids bearing U2-like snRNAs into which changes predicted to restore base pairing had been introduced. Allele-specific suppression of biological and biochemical phenotypes was observed in both cases. Recognition of the UACUAAC box thus relies, at least in part, on Watson-Crick base pairing with the yeast U2 analogue." @default.
• W2100179968 created "2016-06-24" @default.
• W2100179968 creator A5017437583 @default.
• W2100179968 creator A5071683556 @default.
• W2100179968 creator A5086927387 @default.
• W2100179968 date "1987-04-01" @default.
• W2100179968 modified "2023-10-16" @default.
• W2100179968 title "Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA" @default.
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• W2100179968 doi "https://doi.org/10.1016/0092-8674(87)90564-2" @default.
• W2100179968 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3552247" @default.
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