Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100256719> ?p ?o ?g. }
- W2100256719 endingPage "423" @default.
- W2100256719 startingPage "415" @default.
- W2100256719 abstract "Summary. The hepatitis B virus (HBV) replicates via an error-prone reverse transcriptase generating potential drug-resistant quasispecies. The degree of HBV variability in liver vs peripheral blood mononuclear cells (PBMC) in patients on long-term suppressive antivirals is unclear. We characterized HBV replication, drug resistance and molecular diversity in patients with plasma HBV DNA undetectable by clinical assays. Explant liver (n = 9), PBMC (n = 6) and plasma (n = 7) from nine such patients undergoing liver transplantation were evaluated for HBV genomes by sensitive PCR/nucleic acid hybridization assay. Cases with HBV DNA in liver and PBMC were tested for covalently closed circular DNA (HBV cccDNA). HBV polymerase (P) amplicons were cloned, sequenced and both P and overlapping surface (S) gene sequences were analysed. HBV DNA was detected in 43% (3/7) of plasma, 100% (9/9) of liver and 83% (5/6) of PBMC samples. HBV cccDNA was detected in all liver and one PBMC sample. Four patients had a clinical diagnosis of resistance. HBV P gene sequencing revealed 100% wild type (wt) in plasma (2/2), 83% wt in PBMC (5/6) but livers of 3/9 (33%) contained wt and 6/9 (66%) carried resistance to lamivudine and/or adefovir. The translated S gene revealed no changes affecting HBV antigenicity. Sequences from livers with antiviral resistant mutants revealed greater interpatient quasispecies diversity. Despite apparent HBV suppression, the liver continues to support HBV replication and extrahepatic HBV can be detected. PBMC may be a sanctuary for wt virus during antiviral therapy, while the liver harbours more drug-resistant viruses. Drug resistance correlates with intrahepatic viral diversity." @default.
- W2100256719 created "2016-06-24" @default.
- W2100256719 creator A5012272573 @default.
- W2100256719 creator A5019470247 @default.
- W2100256719 creator A5026774458 @default.
- W2100256719 creator A5067220244 @default.
- W2100256719 creator A5071197238 @default.
- W2100256719 creator A5074081305 @default.
- W2100256719 creator A5086633019 @default.
- W2100256719 date "2011-04-24" @default.
- W2100256719 modified "2023-10-05" @default.
- W2100256719 title "Molecular characterization of intrahepatic and extrahepatic hepatitis B virus (HBV) reservoirs in patients on suppressive antiviral therapy" @default.
- W2100256719 cites W1964151379 @default.
- W2100256719 cites W1968526085 @default.
- W2100256719 cites W1986260234 @default.
- W2100256719 cites W1988062715 @default.
- W2100256719 cites W1995085389 @default.
- W2100256719 cites W1999298724 @default.
- W2100256719 cites W2001976371 @default.
- W2100256719 cites W2008241184 @default.
- W2100256719 cites W2010307710 @default.
- W2100256719 cites W2015414255 @default.
- W2100256719 cites W2019586157 @default.
- W2100256719 cites W2021505199 @default.
- W2100256719 cites W2031210994 @default.
- W2100256719 cites W2032455812 @default.
- W2100256719 cites W2032616034 @default.
- W2100256719 cites W2035652598 @default.
- W2100256719 cites W2039736893 @default.
- W2100256719 cites W2040597370 @default.
- W2100256719 cites W2043090634 @default.
- W2100256719 cites W2044965443 @default.
- W2100256719 cites W2051589473 @default.
- W2100256719 cites W2052178073 @default.
- W2100256719 cites W2063847769 @default.
- W2100256719 cites W2068151431 @default.
- W2100256719 cites W2075595885 @default.
- W2100256719 cites W2076506726 @default.
- W2100256719 cites W2079618952 @default.
- W2100256719 cites W2080222370 @default.
- W2100256719 cites W2081812637 @default.
- W2100256719 cites W2081894443 @default.
- W2100256719 cites W2086867812 @default.
- W2100256719 cites W2107863973 @default.
- W2100256719 cites W2110899053 @default.
- W2100256719 cites W2110941134 @default.
- W2100256719 cites W2111255922 @default.
- W2100256719 cites W2113135059 @default.
- W2100256719 cites W2118230692 @default.
- W2100256719 cites W2123774331 @default.
- W2100256719 cites W2125023549 @default.
- W2100256719 cites W2126494596 @default.
- W2100256719 cites W2139610800 @default.
- W2100256719 cites W2142861964 @default.
- W2100256719 cites W2152378881 @default.
- W2100256719 cites W2158240321 @default.
- W2100256719 cites W2166206937 @default.
- W2100256719 cites W2171270480 @default.
- W2100256719 cites W4319704847 @default.
- W2100256719 doi "https://doi.org/10.1111/j.1365-2893.2010.01321.x" @default.
- W2100256719 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4142495" @default.
- W2100256719 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20626626" @default.
- W2100256719 hasPublicationYear "2011" @default.
- W2100256719 type Work @default.
- W2100256719 sameAs 2100256719 @default.
- W2100256719 citedByCount "62" @default.
- W2100256719 countsByYear W21002567192012 @default.
- W2100256719 countsByYear W21002567192013 @default.
- W2100256719 countsByYear W21002567192014 @default.
- W2100256719 countsByYear W21002567192015 @default.
- W2100256719 countsByYear W21002567192016 @default.
- W2100256719 countsByYear W21002567192017 @default.
- W2100256719 countsByYear W21002567192018 @default.
- W2100256719 countsByYear W21002567192019 @default.
- W2100256719 countsByYear W21002567192020 @default.
- W2100256719 countsByYear W21002567192021 @default.
- W2100256719 countsByYear W21002567192023 @default.
- W2100256719 crossrefType "journal-article" @default.
- W2100256719 hasAuthorship W2100256719A5012272573 @default.
- W2100256719 hasAuthorship W2100256719A5019470247 @default.
- W2100256719 hasAuthorship W2100256719A5026774458 @default.
- W2100256719 hasAuthorship W2100256719A5067220244 @default.
- W2100256719 hasAuthorship W2100256719A5071197238 @default.
- W2100256719 hasAuthorship W2100256719A5074081305 @default.
- W2100256719 hasAuthorship W2100256719A5086633019 @default.
- W2100256719 hasBestOaLocation W21002567192 @default.
- W2100256719 hasConcept C126322002 @default.
- W2100256719 hasConcept C137061746 @default.
- W2100256719 hasConcept C14500911 @default.
- W2100256719 hasConcept C153911025 @default.
- W2100256719 hasConcept C159047783 @default.
- W2100256719 hasConcept C189490182 @default.
- W2100256719 hasConcept C202751555 @default.
- W2100256719 hasConcept C2522874641 @default.
- W2100256719 hasConcept C2776408679 @default.
- W2100256719 hasConcept C2777382497 @default.
- W2100256719 hasConcept C2777410769 @default.
- W2100256719 hasConcept C2777869810 @default.