FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100347303> ?p ?o ?g. }

• W2100347303 abstract "Detection and quantification of plant pathogens in the presence of inhibitory substances can be a challenge especially with plant and environmental samples. Real-time quantitative PCR has enabled high-throughput detection and quantification of pathogens; however, its quantitative use is linked to standardized reference materials, and its sensitivity to inhibitors can lead to lower quantification accuracy. Droplet digital PCR has been proposed as a method to overcome these drawbacks. Its absolute quantification does not rely on standards and its tolerance to inhibitors has been demonstrated mostly in clinical samples. Such features would be of great use in agricultural and environmental fields, therefore our study compared the performance of droplet digital PCR method when challenged with inhibitors common to plant and environmental samples and compared it with quantitative PCR. Transfer of an existing Pepper mild mottle virus assay from reverse transcription real-time quantitative PCR to reverse transcription droplet digital PCR was straight forward. When challenged with complex matrices (seeds, plants, soil, wastewater) and selected purified inhibitors droplet digital PCR showed higher resilience to inhibition for the quantification of an RNA virus (Pepper mild mottle virus), compared to reverse transcription real-time quantitative PCR. This study confirms the improved detection and quantification of the PMMoV RT-ddPCR in the presence of inhibitors that are commonly found in samples of seeds, plant material, soil, and wastewater. Together with absolute quantification, independent of standard reference materials, this makes droplet digital PCR a valuable tool for detection and quantification of pathogens in inhibition prone samples." @default.
• W2100347303 created "2016-06-24" @default.
• W2100347303 creator A5020802355 @default.
• W2100347303 creator A5034420465 @default.
• W2100347303 creator A5046418214 @default.
• W2100347303 creator A5067362205 @default.
• W2100347303 creator A5079038168 @default.
• W2100347303 date "2014-12-01" @default.
• W2100347303 modified "2023-10-01" @default.
• W2100347303 title "Reverse transcriptase droplet digital PCR shows high resilience to PCR inhibitors from plant, soil and water samples" @default.
• W2100347303 cites W2016545534 @default.
• W2100347303 cites W2018901651 @default.
• W2100347303 cites W2022091521 @default.
• W2100347303 cites W2025234940 @default.
• W2100347303 cites W2028914711 @default.
• W2100347303 cites W2035613941 @default.
• W2100347303 cites W2038181471 @default.
• W2100347303 cites W2042194227 @default.
• W2100347303 cites W2042547496 @default.
• W2100347303 cites W2045195138 @default.
• W2100347303 cites W2046753373 @default.
• W2100347303 cites W2048237985 @default.
• W2100347303 cites W2058060507 @default.
• W2100347303 cites W2072239957 @default.
• W2100347303 cites W2093403490 @default.
• W2100347303 cites W2099066165 @default.
• W2100347303 cites W2099340333 @default.
• W2100347303 cites W2106058008 @default.
• W2100347303 cites W2112992429 @default.
• W2100347303 cites W2118537438 @default.
• W2100347303 cites W2137175730 @default.
• W2100347303 cites W2138321075 @default.
• W2100347303 cites W2143146123 @default.
• W2100347303 cites W2151575975 @default.
• W2100347303 cites W2159267296 @default.
• W2100347303 cites W2168327674 @default.
• W2100347303 cites W2316397625 @default.
• W2100347303 doi "https://doi.org/10.1186/s13007-014-0042-6" @default.
• W2100347303 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4307183" @default.
• W2100347303 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25628753" @default.
• W2100347303 hasPublicationYear "2014" @default.
• W2100347303 type Work @default.
• W2100347303 sameAs 2100347303 @default.
• W2100347303 citedByCount "165" @default.
• W2100347303 countsByYear W21003473032015 @default.
• W2100347303 countsByYear W21003473032016 @default.
• W2100347303 countsByYear W21003473032017 @default.
• W2100347303 countsByYear W21003473032018 @default.
• W2100347303 countsByYear W21003473032019 @default.
• W2100347303 countsByYear W21003473032020 @default.
• W2100347303 countsByYear W21003473032021 @default.
• W2100347303 countsByYear W21003473032022 @default.
• W2100347303 countsByYear W21003473032023 @default.
• W2100347303 crossrefType "journal-article" @default.
• W2100347303 hasAuthorship W2100347303A5020802355 @default.
• W2100347303 hasAuthorship W2100347303A5034420465 @default.
• W2100347303 hasAuthorship W2100347303A5046418214 @default.
• W2100347303 hasAuthorship W2100347303A5067362205 @default.
• W2100347303 hasAuthorship W2100347303A5079038168 @default.
• W2100347303 hasBestOaLocation W21003473031 @default.
• W2100347303 hasConcept C104317684 @default.
• W2100347303 hasConcept C150194340 @default.
• W2100347303 hasConcept C156719811 @default.
• W2100347303 hasConcept C185592680 @default.
• W2100347303 hasConcept C43617362 @default.
• W2100347303 hasConcept C4692481 @default.
• W2100347303 hasConcept C48023723 @default.
• W2100347303 hasConcept C48126324 @default.
• W2100347303 hasConcept C49105822 @default.
• W2100347303 hasConcept C55493867 @default.
• W2100347303 hasConcept C67705224 @default.
• W2100347303 hasConcept C70721500 @default.
• W2100347303 hasConcept C86803240 @default.
• W2100347303 hasConceptScore W2100347303C104317684 @default.
• W2100347303 hasConceptScore W2100347303C150194340 @default.
• W2100347303 hasConceptScore W2100347303C156719811 @default.
• W2100347303 hasConceptScore W2100347303C185592680 @default.
• W2100347303 hasConceptScore W2100347303C43617362 @default.
• W2100347303 hasConceptScore W2100347303C4692481 @default.
• W2100347303 hasConceptScore W2100347303C48023723 @default.
• W2100347303 hasConceptScore W2100347303C48126324 @default.
• W2100347303 hasConceptScore W2100347303C49105822 @default.
• W2100347303 hasConceptScore W2100347303C55493867 @default.
• W2100347303 hasConceptScore W2100347303C67705224 @default.
• W2100347303 hasConceptScore W2100347303C70721500 @default.
• W2100347303 hasConceptScore W2100347303C86803240 @default.
• W2100347303 hasIssue "1" @default.
• W2100347303 hasLocation W21003473031 @default.
• W2100347303 hasLocation W21003473032 @default.
• W2100347303 hasLocation W21003473033 @default.
• W2100347303 hasLocation W21003473034 @default.
• W2100347303 hasOpenAccess W2100347303 @default.
• W2100347303 hasPrimaryLocation W21003473031 @default.
• W2100347303 hasRelatedWork W118890732 @default.
• W2100347303 hasRelatedWork W1970369006 @default.
• W2100347303 hasRelatedWork W2012602645 @default.
• W2100347303 hasRelatedWork W2957594148 @default.
• W2100347303 hasRelatedWork W2977502671 @default.
• W2100347303 hasRelatedWork W3025384328 @default.
• W2100347303 hasRelatedWork W3184903862 @default.

• next