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- W2100378948 abstract "Genomic DNA was purified from adult female Boophilus annulatus ticks by freezing in liquid nitrogen and grinding followed by Proteinase K digestion, phenol/ chloroform/ isoamyl alcohol and ethanol precipitation. Sequences from the purified DNA were chosen for amplification by PCR with primers chosen on the basis of published B. microplus Bm86 gene, or with primers designed to amplify 0.2 kb, 0.3 kb, 1.3 Kb and 2.2 kb DNA. The four amplicons were cloned in pMOSBlue vector to transform MOSBlue competent cells. The transfected colonies were picked up, propagated to increase the yield of r-plasmids. The r-plasmids were recovered by bacterial cell lysis and linearized by EcoRV restriction enzyme to recover the cloned DNA inserts. Cloning of the DNA inserts in pTARGET expression vector followed by transformation in JM109 competent bacterial cells. The aim was to develop protein expression system of immunogens that can be used as a vaccine against Boophilus annulatus." @default.
- W2100378948 created "2016-06-24" @default.
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- W2100378948 date "2004-01-01" @default.
- W2100378948 modified "2023-09-23" @default.
- W2100378948 title "Approaches towards developing a vaccine against Boophilus annulatus (Acari-Ixodidae): 1-Identification of a gene for expression of gut cell surface proteins" @default.
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