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• W2100386498 abstract "A conductometric biosensor was constructed with a sol-gel immobilized urease on a microfabricated interdigitated array (IDA) gold electrode. Tetramethoxysilane (TMOS) was used as a sol-gel precursor for the immobilization of the urease and the performance of the resulting urea biosensor was controlled through the water content in the acid-catalyzed hydrolysis of the sol-gel stock solution. The sol-gel-derived urea biosensor showed relatively wide dynamic range of 0.2–50 mM in 5 mM imidazole-HC1 buffer at pH 7.5. Furthermore, the urea biosensor exhibited good sensor-to-sensor reproducibility (6.5 %) and storage stability (50 % of its original activity retained after 3 weeks) when stored in 5 mM imidazole-HC1 buffer at 4 °C. The urea biosensor was successfully applied for the urea determination in urine sample by employing the differential measurement format consisting of an active IDA conductometric sensor containing sol-gel-immobilized urease and a reference IDA conductometric sensor containing bovine serum albumin layer instead of the urease." @default.
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• W2100386498 date "2000-01-01" @default.
• W2100386498 modified "2023-09-23" @default.
• W2100386498 title "Microfabricated Conductometric Urea Biosensor Based on Sol-Gel Immobilized Urease" @default.
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• W2100386498 doi "https://doi.org/10.1002/(sici)1521-4109(20000101)12:1<78::aid-elan78>3.0.co;2-b" @default.
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