FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100398370> ?p ?o ?g. }

• W2100398370 endingPage "101" @default.
• W2100398370 startingPage "96" @default.
• W2100398370 abstract "ABSTRACT The adherence of Porphyromonas gingivalis to host cells is likely a prerequisite step in the pathogenesis of P. gingivalis -induced periodontal disease. P. gingivalis binds to and invades epithelial cells, and fimbriae are shown to be involved in this process. Little is known regarding epithelial receptor(s) involved in binding of P. gingivalis fimbriae. Using an overlay assay with purified P. gingivalis fimbriae as a probe, two major epithelial cell proteins with masses of 50 and 40 kDa were identified by immunoblotting with fimbria-specific antibodies. Iodinated purified fimbriae also bound to the same two epithelial cell proteins. An affinity chromatography technique was utilized to isolate and purify the epithelial components to which P. gingivalis fimbriae bind. Purified fimbriae were coupled to CNBr-activated Sepharose-4B, and the solubilized epithelial cell extract proteins bound to the immobilized fimbriae were isolated from the column. A major 50-kDa component and a minor 40-kDa component were purified and could be digested with trypsin, suggesting that they were proteins. These affinity-eluted 50- and 40-kDa proteins were then subjected to amino-terminal sequencing, and no sequence could be determined, suggesting that these proteins have blocked amino-terminal residues. CNBr digestion of the 50-kDa component resulted in an internal sequence homologous to that of Keratin I molecules. Further evidence that P. gingivalis fimbriae bind to cytokeratin molecule(s) comes from studies showing that multicytokeratin rabbit polyclonal antibodies cross-react with the affinity-purified 50-kDa epithelial cell surface component. Also, binding of purified P. gingivalis fimbriae to epithelial components can be inhibited in an overlay assay by multicytokeratin rabbit polyclonal antibodies. Furthermore, we showed that biotinylated purified fimbriae bind to purified human epidermal keratin in an overlay assay. These studies suggest that the surface-accessible epithelial cytokeratins may act as receptor(s) for P. gingivalis fimbriae. We hypothesize that adherence of P. gingivalis fimbriae to cytokeratin may be important for colonization of oral mucous membranes and possibly also for activation of epithelial cells." @default.
• W2100398370 created "2016-06-24" @default.
• W2100398370 creator A5019676251 @default.
• W2100398370 creator A5050435224 @default.
• W2100398370 creator A5090175941 @default.
• W2100398370 date "2002-01-01" @default.
• W2100398370 modified "2023-09-27" @default.
• W2100398370 title "<i>Porphyromonas gingivalis</i> Fimbriae Bind to Cytokeratin of Epithelial Cells" @default.
• W2100398370 cites W1508068435 @default.
• W2100398370 cites W1544973994 @default.
• W2100398370 cites W1591346024 @default.
• W2100398370 cites W167158724 @default.
• W2100398370 cites W1785582311 @default.
• W2100398370 cites W1790840706 @default.
• W2100398370 cites W1961692317 @default.
• W2100398370 cites W1967120118 @default.
• W2100398370 cites W1981077894 @default.
• W2100398370 cites W1984785242 @default.
• W2100398370 cites W1998735143 @default.
• W2100398370 cites W2018793626 @default.
• W2100398370 cites W2020406808 @default.
• W2100398370 cites W2033785962 @default.
• W2100398370 cites W2058689337 @default.
• W2100398370 cites W2084266000 @default.
• W2100398370 cites W2101108802 @default.
• W2100398370 cites W2106067571 @default.
• W2100398370 cites W2111125148 @default.
• W2100398370 cites W2113949809 @default.
• W2100398370 cites W2141827258 @default.
• W2100398370 cites W2142200441 @default.
• W2100398370 cites W2144517739 @default.
• W2100398370 cites W2145054467 @default.
• W2100398370 cites W2149914301 @default.
• W2100398370 cites W2161126388 @default.
• W2100398370 cites W2167491944 @default.
• W2100398370 cites W2189002985 @default.
• W2100398370 cites W4250547753 @default.
• W2100398370 doi "https://doi.org/10.1128/iai.70.1.96-101.2002" @default.
• W2100398370 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/127629" @default.
• W2100398370 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11748168" @default.
• W2100398370 hasPublicationYear "2002" @default.
• W2100398370 type Work @default.
• W2100398370 sameAs 2100398370 @default.
• W2100398370 citedByCount "57" @default.
• W2100398370 countsByYear W21003983702012 @default.
• W2100398370 countsByYear W21003983702013 @default.
• W2100398370 countsByYear W21003983702014 @default.
• W2100398370 countsByYear W21003983702015 @default.
• W2100398370 countsByYear W21003983702017 @default.
• W2100398370 countsByYear W21003983702019 @default.
• W2100398370 countsByYear W21003983702021 @default.
• W2100398370 countsByYear W21003983702022 @default.
• W2100398370 crossrefType "journal-article" @default.
• W2100398370 hasAuthorship W2100398370A5019676251 @default.
• W2100398370 hasAuthorship W2100398370A5050435224 @default.
• W2100398370 hasAuthorship W2100398370A5090175941 @default.
• W2100398370 hasBestOaLocation W21003983701 @default.
• W2100398370 hasConcept C104317684 @default.
• W2100398370 hasConcept C153911025 @default.
• W2100398370 hasConcept C159654299 @default.
• W2100398370 hasConcept C203014093 @default.
• W2100398370 hasConcept C2776357993 @default.
• W2100398370 hasConcept C2780183776 @default.
• W2100398370 hasConcept C2910768159 @default.
• W2100398370 hasConcept C39103817 @default.
• W2100398370 hasConcept C523546767 @default.
• W2100398370 hasConcept C54355233 @default.
• W2100398370 hasConcept C547475151 @default.
• W2100398370 hasConcept C55493867 @default.
• W2100398370 hasConcept C86803240 @default.
• W2100398370 hasConcept C89423630 @default.
• W2100398370 hasConcept C956191 @default.
• W2100398370 hasConceptScore W2100398370C104317684 @default.
• W2100398370 hasConceptScore W2100398370C153911025 @default.
• W2100398370 hasConceptScore W2100398370C159654299 @default.
• W2100398370 hasConceptScore W2100398370C203014093 @default.
• W2100398370 hasConceptScore W2100398370C2776357993 @default.
• W2100398370 hasConceptScore W2100398370C2780183776 @default.
• W2100398370 hasConceptScore W2100398370C2910768159 @default.
• W2100398370 hasConceptScore W2100398370C39103817 @default.
• W2100398370 hasConceptScore W2100398370C523546767 @default.
• W2100398370 hasConceptScore W2100398370C54355233 @default.
• W2100398370 hasConceptScore W2100398370C547475151 @default.
• W2100398370 hasConceptScore W2100398370C55493867 @default.
• W2100398370 hasConceptScore W2100398370C86803240 @default.
• W2100398370 hasConceptScore W2100398370C89423630 @default.
• W2100398370 hasConceptScore W2100398370C956191 @default.
• W2100398370 hasIssue "1" @default.
• W2100398370 hasLocation W21003983701 @default.
• W2100398370 hasLocation W21003983702 @default.
• W2100398370 hasLocation W21003983703 @default.
• W2100398370 hasOpenAccess W2100398370 @default.
• W2100398370 hasPrimaryLocation W21003983701 @default.
• W2100398370 hasRelatedWork W1508068435 @default.
• W2100398370 hasRelatedWork W1920685537 @default.
• W2100398370 hasRelatedWork W1994699969 @default.
• W2100398370 hasRelatedWork W2001837605 @default.
• W2100398370 hasRelatedWork W2009378504 @default.

• next