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• W2100456890 abstract "Tobacco mosaic tobamovirus (TMV) is one of the most destructive virus threatening worldwide tobacco production. Use of host resistance is the best method of control. The N -gene was introgressed into tobacco from Nicotiana glutinosa to confer hypersensitive resistance to TMV. Phenotypic selection of TMV resistant germplasm is expensive, slow and unreliable. Use of N -gene specific primers is efficient in selecting TMV resistant germplasm in marker-assisted breeding. This study aimed at assessing the utility of N -gene specific primers in flue cured and dark-fire cured tobacco breeding materials in Zimbabwe. Four specific primers namely N1/N2, AS1/AS2, E1/E2 and SD1/SD2 were used to detect the N -gene in flue cured and dark-fire cured tobacco. DNA was extracted from young leaves of tobacco plants and quantified by a spectrophotometer. Polymerase chain reaction (PCR) mix and amplifying conditions for the four specific primer pairs were optimized. Results show that out of the four sets of primers used, AS1/AS2 and SD1/SD2 did not produce expected band products, while N1/N2 and E1/E2 detected the N -gene in flue cured and dark-fire cured tobacco. Therefore, the use of N1/N2 and E1/E2 primers will be relatively cheap, effective and quick in the foreground selection of the N -gene. Keywords: N -gene, specific primers, resistance, molecular markers, Nicotiana tabacum . African Journal of Biotechnology Vol. 12(24), pp. 3783-3789" @default.
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• W2100456890 date "2013-06-12" @default.
• W2100456890 modified "2023-09-28" @default.
• W2100456890 title "Potential of marker-assisted selection for Tobacco mosaic tobamovirus resistance in tobacco breeding" @default.
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• W2100456890 doi "https://doi.org/10.5897/ajb2013.12309" @default.
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