FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100459042> ?p ?o ?g. }

• W2100459042 endingPage "12" @default.
• W2100459042 startingPage "12" @default.
• W2100459042 abstract "Long-terminal repeat (LTR) retrotransposons have complex modes of mobility involving reverse transcription of their RNA genomes in cytoplasmic virus-like particles (VLPs) and integration of the cDNA copies into the host genome. The limited coding capacity of retrotransposons necessitates an extensive reliance on host co-factors; however, it has been challenging to identify co-factors that are required for endogenous retrotransposon mobility because retrotransposition is such a rare event. To circumvent the low frequency of Ty1 LTR-retrotransposon mobility in Saccharomyces cerevisiae, we used iterative synthetic genetic array (SGA) analysis to isolate host mutations that reduce retrotransposition. Query strains that harbor a chromosomal Ty1his3AI reporter element and either the rtt101Δ or med1Δ mutation, both of which confer a hypertransposition phenotype, were mated to 4,847 haploid ORF deletion strains. Retrotransposition was measured in the double mutant progeny, and a set of 275 ORF deletions that suppress the hypertransposition phenotypes of both rtt101Δ and med1Δ were identified. The corresponding set of 275 retrotransposition host factors (RHFs) includes 45 previously identified Ty1 or Ty3 co-factors. More than half of the RHF genes have statistically robust human homologs (E < 1 x 10-10). The level of unintegrated Ty1 cDNA in 181 rhfΔ single mutants was altered <2-fold, suggesting that the corresponding co-factors stimulate retrotransposition at a step after cDNA synthesis. However, deletion of 43 RHF genes, including specific ribosomal protein and ribosome biogenesis genes and RNA degradation, modification and transport genes resulted in low Ty1 cDNA levels. The level of Ty1 Gag but not RNA was reduced in ribosome biogenesis mutants bud21Δ, hcr1Δ, loc1Δ, and puf6Δ. Ty1 retrotransposition is dependent on multiple co-factors acting at different steps in the replication cycle. Human orthologs of these RHFs are potential, or in a few cases, presumptive HIV-1 co-factors in human cells. RHF genes whose absence results in decreased Ty1 cDNA include characterized RNA metabolism and modification genes, consistent with their having roles in early steps in retrotransposition such as expression, nuclear export, translation, localization, or packaging of Ty1 RNA. Our results suggest that Bud21, Hcr1, Loc1, and Puf6 promote efficient synthesis or stability of Ty1 Gag." @default.
• W2100459042 created "2016-06-24" @default.
• W2100459042 creator A5003536421 @default.
• W2100459042 creator A5070054150 @default.
• W2100459042 creator A5072512858 @default.
• W2100459042 creator A5077466253 @default.
• W2100459042 creator A5080249499 @default.
• W2100459042 date "2012-01-01" @default.
• W2100459042 modified "2023-10-15" @default.
• W2100459042 title "Host co-factors of the retrovirus-like transposon Ty1" @default.
• W2100459042 cites W1492171676 @default.
• W2100459042 cites W1587089803 @default.
• W2100459042 cites W1601258312 @default.
• W2100459042 cites W1858119869 @default.
• W2100459042 cites W1952178886 @default.
• W2100459042 cites W1953861638 @default.
• W2100459042 cites W1968977901 @default.
• W2100459042 cites W1969855294 @default.
• W2100459042 cites W1972703973 @default.
• W2100459042 cites W1972852906 @default.
• W2100459042 cites W1976539988 @default.
• W2100459042 cites W1978141733 @default.
• W2100459042 cites W1978895170 @default.
• W2100459042 cites W1979039733 @default.
• W2100459042 cites W1981531064 @default.
• W2100459042 cites W1982741055 @default.
• W2100459042 cites W1984158753 @default.
• W2100459042 cites W2000254114 @default.
• W2100459042 cites W2000805168 @default.
• W2100459042 cites W2001876820 @default.
• W2100459042 cites W2003730339 @default.
• W2100459042 cites W2006018650 @default.
• W2100459042 cites W2007998870 @default.
• W2100459042 cites W2013303176 @default.
• W2100459042 cites W2013706075 @default.
• W2100459042 cites W2022473619 @default.
• W2100459042 cites W2023420720 @default.
• W2100459042 cites W2029388127 @default.
• W2100459042 cites W2030256602 @default.
• W2100459042 cites W2035865387 @default.
• W2100459042 cites W2043249063 @default.
• W2100459042 cites W2045667084 @default.
• W2100459042 cites W2045862142 @default.
• W2100459042 cites W2046722451 @default.
• W2100459042 cites W2050228332 @default.
• W2100459042 cites W2051541780 @default.
• W2100459042 cites W2063490358 @default.
• W2100459042 cites W2063587388 @default.
• W2100459042 cites W2067552255 @default.
• W2100459042 cites W2068880841 @default.
• W2100459042 cites W2072080280 @default.
• W2100459042 cites W2072083511 @default.
• W2100459042 cites W2084509375 @default.
• W2100459042 cites W2090077122 @default.
• W2100459042 cites W2093373040 @default.
• W2100459042 cites W2100152753 @default.
• W2100459042 cites W2101107183 @default.
• W2100459042 cites W2109997253 @default.
• W2100459042 cites W2114192711 @default.
• W2100459042 cites W2119596584 @default.
• W2100459042 cites W2122713795 @default.
• W2100459042 cites W2130024434 @default.
• W2100459042 cites W2131194288 @default.
• W2100459042 cites W2132665479 @default.
• W2100459042 cites W2136312358 @default.
• W2100459042 cites W2137472791 @default.
• W2100459042 cites W2140152984 @default.
• W2100459042 cites W2143341850 @default.
• W2100459042 cites W2146140517 @default.
• W2100459042 cites W2146503498 @default.
• W2100459042 cites W2152722934 @default.
• W2100459042 cites W2153606842 @default.
• W2100459042 cites W2158963222 @default.
• W2100459042 cites W2159460045 @default.
• W2100459042 cites W2161409320 @default.
• W2100459042 cites W2162828523 @default.
• W2100459042 cites W2163198099 @default.
• W2100459042 cites W2163796139 @default.
• W2100459042 cites W2165178639 @default.
• W2100459042 cites W2166077200 @default.
• W2100459042 cites W2167734189 @default.
• W2100459042 cites W2170812692 @default.
• W2100459042 cites W2170863549 @default.
• W2100459042 cites W2171792346 @default.
• W2100459042 cites W2172042920 @default.
• W2100459042 cites W2764789340 @default.
• W2100459042 cites W629335773 @default.
• W2100459042 cites W2739239841 @default.
• W2100459042 doi "https://doi.org/10.1186/1759-8753-3-12" @default.
• W2100459042 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3522557" @default.
• W2100459042 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22856544" @default.
• W2100459042 hasPublicationYear "2012" @default.
• W2100459042 type Work @default.
• W2100459042 sameAs 2100459042 @default.
• W2100459042 citedByCount "32" @default.
• W2100459042 countsByYear W21004590422014 @default.
• W2100459042 countsByYear W21004590422015 @default.
• W2100459042 countsByYear W21004590422016 @default.

• next