Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100459505> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2100459505 endingPage "44" @default.
- W2100459505 startingPage "35" @default.
- W2100459505 abstract "Monoclonal antibodies (mAbs) against Actinobacillus pleuropneumoniae serotype 2 (reference strain Shope 4226 and field isolate F46) were produced. Twelve hybridoma clones were selected against both strains, and all the antibodies secreted were found to be reactive with whole-cell antigen of the homologous strain in ELISA, whereas only one mAb was reactive in slide agglutination test. The predominant antibody classes were IgG2b and IgG3, although IgG1 and IgM were also obtained. Immunoblot assay showed that mAbs could recognize a ladder band profile which is in accordance with the O-antigen of lipopolysaccharide. Most of the epitopes involved were resistant to proteinase K and also to boiling in the presence of sodium dodecyl sulfate and reducing conditions, but they were sensitive to periodic acid. The 12 mAbs recognized neither reference strains of the remaining A. pleuropneumoniae serotypes nor other taxonomically related Gram-negative organisms. The suitability of mAbs for serotyping of field isolates was also examined, and a high correlation (97.4%) was found between the results previously established by indirect hemagglutination with polyclonal rabbit sera and those obtained by ELISA with mAbs. The panel of mAbs described in this study was found to be extremely useful for identifying field isolates belonging to serotype 2 and could be used as a complementary serotyping method." @default.
- W2100459505 created "2016-06-24" @default.
- W2100459505 creator A5039066982 @default.
- W2100459505 creator A5043851987 @default.
- W2100459505 creator A5051217733 @default.
- W2100459505 creator A5079383946 @default.
- W2100459505 creator A5081321172 @default.
- W2100459505 creator A5089458290 @default.
- W2100459505 date "1995-03-01" @default.
- W2100459505 modified "2023-09-27" @default.
- W2100459505 title "Characterization of monoclonal antibodies to O-antigen of lipopolysaccharide ofActinobacillus pleuropneumoniaeserotype 2 and their use in the classification of field isolates" @default.
- W2100459505 cites W1605306231 @default.
- W2100459505 cites W1754615032 @default.
- W2100459505 cites W177015965 @default.
- W2100459505 cites W1922098314 @default.
- W2100459505 cites W1971621578 @default.
- W2100459505 cites W2026443485 @default.
- W2100459505 cites W2027616634 @default.
- W2100459505 cites W2028290631 @default.
- W2100459505 cites W2030522563 @default.
- W2100459505 cites W2036430011 @default.
- W2100459505 cites W2079026396 @default.
- W2100459505 cites W2082903549 @default.
- W2100459505 cites W2136033496 @default.
- W2100459505 cites W2147370341 @default.
- W2100459505 cites W2413871895 @default.
- W2100459505 cites W2416879522 @default.
- W2100459505 cites W4293247451 @default.
- W2100459505 cites W92814152 @default.
- W2100459505 doi "https://doi.org/10.1111/j.1574-695x.1995.tb00076.x" @default.
- W2100459505 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7541271" @default.
- W2100459505 hasPublicationYear "1995" @default.
- W2100459505 type Work @default.
- W2100459505 sameAs 2100459505 @default.
- W2100459505 citedByCount "2" @default.
- W2100459505 crossrefType "journal-article" @default.
- W2100459505 hasAuthorship W2100459505A5039066982 @default.
- W2100459505 hasAuthorship W2100459505A5043851987 @default.
- W2100459505 hasAuthorship W2100459505A5051217733 @default.
- W2100459505 hasAuthorship W2100459505A5079383946 @default.
- W2100459505 hasAuthorship W2100459505A5081321172 @default.
- W2100459505 hasAuthorship W2100459505A5089458290 @default.
- W2100459505 hasBestOaLocation W21004595051 @default.
- W2100459505 hasConcept C10389963 @default.
- W2100459505 hasConcept C143508840 @default.
- W2100459505 hasConcept C147483822 @default.
- W2100459505 hasConcept C159047783 @default.
- W2100459505 hasConcept C159654299 @default.
- W2100459505 hasConcept C195616568 @default.
- W2100459505 hasConcept C203014093 @default.
- W2100459505 hasConcept C2779026604 @default.
- W2100459505 hasConcept C2780861368 @default.
- W2100459505 hasConcept C34343649 @default.
- W2100459505 hasConcept C45189115 @default.
- W2100459505 hasConcept C542903549 @default.
- W2100459505 hasConcept C73878171 @default.
- W2100459505 hasConcept C86803240 @default.
- W2100459505 hasConcept C89423630 @default.
- W2100459505 hasConcept C956191 @default.
- W2100459505 hasConceptScore W2100459505C10389963 @default.
- W2100459505 hasConceptScore W2100459505C143508840 @default.
- W2100459505 hasConceptScore W2100459505C147483822 @default.
- W2100459505 hasConceptScore W2100459505C159047783 @default.
- W2100459505 hasConceptScore W2100459505C159654299 @default.
- W2100459505 hasConceptScore W2100459505C195616568 @default.
- W2100459505 hasConceptScore W2100459505C203014093 @default.
- W2100459505 hasConceptScore W2100459505C2779026604 @default.
- W2100459505 hasConceptScore W2100459505C2780861368 @default.
- W2100459505 hasConceptScore W2100459505C34343649 @default.
- W2100459505 hasConceptScore W2100459505C45189115 @default.
- W2100459505 hasConceptScore W2100459505C542903549 @default.
- W2100459505 hasConceptScore W2100459505C73878171 @default.
- W2100459505 hasConceptScore W2100459505C86803240 @default.
- W2100459505 hasConceptScore W2100459505C89423630 @default.
- W2100459505 hasConceptScore W2100459505C956191 @default.
- W2100459505 hasIssue "1" @default.
- W2100459505 hasLocation W21004595051 @default.
- W2100459505 hasLocation W21004595052 @default.
- W2100459505 hasOpenAccess W2100459505 @default.
- W2100459505 hasPrimaryLocation W21004595051 @default.
- W2100459505 hasRelatedWork W1483259183 @default.
- W2100459505 hasRelatedWork W2002744208 @default.
- W2100459505 hasRelatedWork W2002752743 @default.
- W2100459505 hasRelatedWork W2036320803 @default.
- W2100459505 hasRelatedWork W2100459505 @default.
- W2100459505 hasRelatedWork W2146703554 @default.
- W2100459505 hasRelatedWork W2386118910 @default.
- W2100459505 hasRelatedWork W2403172758 @default.
- W2100459505 hasRelatedWork W2412025079 @default.
- W2100459505 hasRelatedWork W3203022778 @default.
- W2100459505 hasVolume "11" @default.
- W2100459505 isParatext "false" @default.
- W2100459505 isRetracted "false" @default.
- W2100459505 magId "2100459505" @default.
- W2100459505 workType "article" @default.