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- W2100463719 abstract "A simple design of a label-free colorimetric sensor for coralyne was demonstrated, which is based on the coralyne-modulated peroxidase-like split G-quadruplex (split G4) DNAzyme via A2–coralyne–A2 coordination. In the present work, two specific oligonucleotide strands were used. Upon addition of coralyne, it could specifically bind to A18 sequences with an apparent binding constant of 1.05 × 105 M−1, which induced the formation of an anti-parallel homo-adenine duplex, accompanied by the proximity of two G-rich sequences responsible for the formation of split G4. In the presence of hemin, this split G4 was able to catalyze the H2O2-mediated oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic-acid) diammonium salt (ABTS–H2O2 system), resulting in a maximal absorbance at approximately 420 nm. A linear response from 0.06 to 10 μM was obtained for coralyne, with a linear correlation coefficient of 0.9987. The detection limit was estimated to be 19 nM based on a S/N of 3." @default.
- W2100463719 created "2016-06-24" @default.
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- W2100463719 date "2013-01-01" @default.
- W2100463719 modified "2023-10-02" @default.
- W2100463719 title "Label-free colorimetric detection of coralyne utilizing peroxidase-like split G-quadruplex DNAzyme" @default.
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- W2100463719 doi "https://doi.org/10.1039/c3ay40796a" @default.
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