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• W2100510491 abstract "A method is described for the estimation of the true width of fibrillar or rod-like structures from electron micrographs of metal-shadowed preparations. The method is based on variations in the image width as a function of the angle (ß) between the long axis of the fibril and the direction of the shadow in the plane of the preparation. The image width when ß = 0° practically represents the real width of the elongated particle but is often indistinguishable from the background. The fibril image width is conveniently measured at ß values between 15° and 90°. The true width is obtained by plotting the image width versus sin ß and extrapolating to ß = 0°. Latex spheres are sprayed with the fibrils or rods to indicate the direction of shadow. Tobacco mosaic virus (TMV) was used as a model structure because of its known constant diameter of 150 A (5). The width (in the case of TMV equal to the diameter) found by the present method was 150 A ± 8 A." @default.
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• W2100510491 date "1963-05-01" @default.
• W2100510491 modified "2023-10-16" @default.
• W2100510491 title "THE USE OF SHADOW-CASTING TECHNIQUE FOR MEASUREMENT OF THE WIDTH OF ELONGATED PARTICLES" @default.
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• W2100510491 doi "https://doi.org/10.1083/jcb.17.2.321" @default.
• W2100510491 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2106203" @default.
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