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- W2100589600 endingPage "e20489" @default.
- W2100589600 startingPage "e20489" @default.
- W2100589600 abstract "Mitosis is a fundamental process in the development of all organisms. The mitotic spindle guides the cell through mitosis as it mediates the segregation of chromosomes, the orientation of the cleavage furrow, and the progression of cell division. Birth defects and tissue-specific cancers often result from abnormalities in mitotic events. Here, we report a proteomic study of the mitotic spindle from Chinese Hamster Ovary (CHO) cells. Four different isolations of metaphase spindles were subjected to Multi-dimensional Protein Identification Technology (MudPIT) analysis and tandem mass spectrometry. We identified 1155 proteins and used Gene Ontology (GO) analysis to categorize proteins into cellular component groups. We then compared our data to the previously published CHO midbody proteome and identified proteins that are unique to the CHO spindle. Our data represent the first mitotic spindle proteome in CHO cells, which augments the list of mitotic spindle components from mammalian cells." @default.
- W2100589600 created "2016-06-24" @default.
- W2100589600 creator A5019665230 @default.
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- W2100589600 creator A5060754111 @default.
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- W2100589600 date "2011-05-27" @default.
- W2100589600 modified "2023-10-14" @default.
- W2100589600 title "Mitotic Spindle Proteomics in Chinese Hamster Ovary Cells" @default.
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- W2100589600 doi "https://doi.org/10.1371/journal.pone.0020489" @default.
- W2100589600 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3103581" @default.
- W2100589600 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21647379" @default.
- W2100589600 hasPublicationYear "2011" @default.
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