Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100598464> ?p ?o ?g. }
- W2100598464 endingPage "605" @default.
- W2100598464 startingPage "593" @default.
- W2100598464 abstract "Fibroblast growth factor 3 (FGF3) was originally identified as the mouse proto-oncogene Int-2, which is activated by proviral insertion in tumors induced by mouse mammary tumor virus. To facilitate the biological characterization of the ligand, we have analyzed its homologue in Xenopus laevis, XFGF3. Here we confirm that the X. laevis genome contains two distinct FGF3 alleles, neither of which is capable of encoding the NH2-terminally extended forms specified by the mouse and human FGF3 genes. Unlike the mammalian proteins, XFGF3 is efficiently secreted as a Mr 31,000 glycoprotein, gp31, which undergoes proteolytic cleavage to produce an NH2-terminally truncated product, gp27. Processing removes a segment of 18 amino acids immediately distal to the signal peptide that is not present in the mammalian homologues. By inserting an epitope-tag adjacent to the cleavage site, we show that a substantial amount of the gp27 is generated intracellularly, although processing can also occur in the extracellular matrix. Two residues are also removed from the COOH terminus. To compare the biological properties of the different forms, cDNAs were constructed that selectively give rise to the larger, gp31, or smaller, gp27, forms of XFGF3. As judged by their ability to cause morphological transformation of NIH3T3 cells, their mitogenicity on specific cell types, and their affinity for the IIIb and IIIc isoforms of Xenopus FGF receptors, gp27 has a much higher biological activity than gp31. Sequence comparison revealed an intriguing similar cleavage motif immediately downstream of the signal peptide cleavage site in the NH2-terminus of mouse and human FGF3. Analysis of secreted mutant mouse FGF3 confirmed an additional NH2-terminal processing at the corresponding sequence motif. NH2-terminal trimming of Xenopus and mammalian FGF3s may therefore be a prerequisite of optimal biological activity." @default.
- W2100598464 created "2016-06-24" @default.
- W2100598464 creator A5012669553 @default.
- W2100598464 creator A5026207602 @default.
- W2100598464 creator A5036312326 @default.
- W2100598464 creator A5051758709 @default.
- W2100598464 creator A5080435657 @default.
- W2100598464 creator A5088156880 @default.
- W2100598464 creator A5091718117 @default.
- W2100598464 date "2000-11-01" @default.
- W2100598464 modified "2023-09-23" @default.
- W2100598464 title "NH2-terminal cleavage of xenopus fibroblast growth factor 3 is necessary for optimal biological activity and receptor binding." @default.
- W2100598464 cites W132416300 @default.
- W2100598464 cites W1432640003 @default.
- W2100598464 cites W1495121347 @default.
- W2100598464 cites W1498373517 @default.
- W2100598464 cites W1499670070 @default.
- W2100598464 cites W1513451331 @default.
- W2100598464 cites W1514636046 @default.
- W2100598464 cites W1592656278 @default.
- W2100598464 cites W1799346688 @default.
- W2100598464 cites W1826524642 @default.
- W2100598464 cites W1854803455 @default.
- W2100598464 cites W1928956084 @default.
- W2100598464 cites W1933680278 @default.
- W2100598464 cites W1937327614 @default.
- W2100598464 cites W1949259756 @default.
- W2100598464 cites W1953638641 @default.
- W2100598464 cites W1981718544 @default.
- W2100598464 cites W1985457327 @default.
- W2100598464 cites W1993531220 @default.
- W2100598464 cites W1996294835 @default.
- W2100598464 cites W1998131878 @default.
- W2100598464 cites W2024180542 @default.
- W2100598464 cites W2026178507 @default.
- W2100598464 cites W2034534896 @default.
- W2100598464 cites W2043328403 @default.
- W2100598464 cites W2055114143 @default.
- W2100598464 cites W2058525519 @default.
- W2100598464 cites W2079583639 @default.
- W2100598464 cites W2082012658 @default.
- W2100598464 cites W2082528610 @default.
- W2100598464 cites W2082842791 @default.
- W2100598464 cites W2083186952 @default.
- W2100598464 cites W2088775512 @default.
- W2100598464 cites W2090040908 @default.
- W2100598464 cites W2102768625 @default.
- W2100598464 cites W2117928953 @default.
- W2100598464 cites W2118830070 @default.
- W2100598464 cites W2123924871 @default.
- W2100598464 cites W2128897447 @default.
- W2100598464 cites W2138773713 @default.
- W2100598464 cites W2149356058 @default.
- W2100598464 cites W2149738106 @default.
- W2100598464 cites W2150400066 @default.
- W2100598464 cites W2158228414 @default.
- W2100598464 cites W2159175125 @default.
- W2100598464 cites W2168847939 @default.
- W2100598464 cites W2183350970 @default.
- W2100598464 cites W2215417236 @default.
- W2100598464 cites W230344177 @default.
- W2100598464 cites W2337645172 @default.
- W2100598464 cites W2406309171 @default.
- W2100598464 cites W2417999762 @default.
- W2100598464 cites W2470905000 @default.
- W2100598464 cites W67169912 @default.
- W2100598464 cites W748860 @default.
- W2100598464 cites W79330750 @default.
- W2100598464 cites W81411684 @default.
- W2100598464 cites W84045147 @default.
- W2100598464 cites W958175540 @default.
- W2100598464 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11095248" @default.
- W2100598464 hasPublicationYear "2000" @default.
- W2100598464 type Work @default.
- W2100598464 sameAs 2100598464 @default.
- W2100598464 citedByCount "1" @default.
- W2100598464 crossrefType "journal-article" @default.
- W2100598464 hasAuthorship W2100598464A5012669553 @default.
- W2100598464 hasAuthorship W2100598464A5026207602 @default.
- W2100598464 hasAuthorship W2100598464A5036312326 @default.
- W2100598464 hasAuthorship W2100598464A5051758709 @default.
- W2100598464 hasAuthorship W2100598464A5080435657 @default.
- W2100598464 hasAuthorship W2100598464A5088156880 @default.
- W2100598464 hasAuthorship W2100598464A5091718117 @default.
- W2100598464 hasConcept C104317684 @default.
- W2100598464 hasConcept C10858879 @default.
- W2100598464 hasConcept C108625454 @default.
- W2100598464 hasConcept C151730666 @default.
- W2100598464 hasConcept C153911025 @default.
- W2100598464 hasConcept C159654299 @default.
- W2100598464 hasConcept C167625842 @default.
- W2100598464 hasConcept C170493617 @default.
- W2100598464 hasConcept C175156509 @default.
- W2100598464 hasConcept C195616568 @default.
- W2100598464 hasConcept C2779281246 @default.
- W2100598464 hasConcept C2779535977 @default.
- W2100598464 hasConcept C43369102 @default.
- W2100598464 hasConcept C53345823 @default.