FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100648049> ?p ?o ?g. }

• W2100648049 endingPage "740" @default.
• W2100648049 startingPage "731" @default.
• W2100648049 abstract "The tumor suppressor p53 is conformationally unstable at physiological temperature. Even the activated p53delta30 variant, which lacks the self-inhibiting carboxy terminal domain, has a half-life of only 8 min at 37 degrees C in vitro. We have developed a genetic approach to identify p53 variants that stabilize the active conformation. The human p53delta30 gene was randomly mutated, and the resulting library was expressed in Escherichia coli under conditions that apparently denatured the parental protein. Stable p53 variants were identified based on their ability to specifically bind a p53 consensus site. The initial thermostable variants were randomly recombined by DNA shuffling, and substitutions that were functionally additive or synergistic were identified in a second more stringent round of screening. The DNA binding activity of N239Y/N268D/E336V p53delta30 variant has a half-life of 100 min at 37 degrees C, 12 times longer than that of the parental protein. The thermostable variants should be more amenable to crystallographic studies and more effective in gene therapies than the wild-type protein." @default.
• W2100648049 created "2016-06-24" @default.
• W2100648049 creator A5008641700 @default.
• W2100648049 creator A5089627980 @default.
• W2100648049 date "2008-12-31" @default.
• W2100648049 modified "2023-09-26" @default.
• W2100648049 title "In vitro evolution of thermostable p53 variants" @default.
• W2100648049 cites W1903324674 @default.
• W2100648049 cites W1971966311 @default.
• W2100648049 cites W1986233163 @default.
• W2100648049 cites W1990406980 @default.
• W2100648049 cites W1991494214 @default.
• W2100648049 cites W1992456676 @default.
• W2100648049 cites W1996899753 @default.
• W2100648049 cites W1999851893 @default.
• W2100648049 cites W2003236156 @default.
• W2100648049 cites W2006607764 @default.
• W2100648049 cites W2012183441 @default.
• W2100648049 cites W2015006770 @default.
• W2100648049 cites W2015574756 @default.
• W2100648049 cites W2020527010 @default.
• W2100648049 cites W2030091498 @default.
• W2100648049 cites W2033806655 @default.
• W2100648049 cites W2042316019 @default.
• W2100648049 cites W2051182140 @default.
• W2100648049 cites W205240979 @default.
• W2100648049 cites W2062202414 @default.
• W2100648049 cites W2068691401 @default.
• W2100648049 cites W2071086763 @default.
• W2100648049 cites W2074222004 @default.
• W2100648049 cites W2081115041 @default.
• W2100648049 cites W2089289562 @default.
• W2100648049 cites W2093059615 @default.
• W2100648049 cites W2096276671 @default.
• W2100648049 cites W2126679757 @default.
• W2100648049 cites W2135636849 @default.
• W2100648049 cites W2147620559 @default.
• W2100648049 cites W2150710841 @default.
• W2100648049 cites W2359374960 @default.
• W2100648049 cites W2404990193 @default.
• W2100648049 cites W2407819947 @default.
• W2100648049 doi "https://doi.org/10.1110/ps.8.4.731" @default.
• W2100648049 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2144309" @default.
• W2100648049 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10211819" @default.
• W2100648049 hasPublicationYear "2008" @default.
• W2100648049 type Work @default.
• W2100648049 sameAs 2100648049 @default.
• W2100648049 citedByCount "28" @default.
• W2100648049 countsByYear W21006480492012 @default.
• W2100648049 countsByYear W21006480492014 @default.
• W2100648049 countsByYear W21006480492015 @default.
• W2100648049 countsByYear W21006480492016 @default.
• W2100648049 countsByYear W21006480492020 @default.
• W2100648049 crossrefType "journal-article" @default.
• W2100648049 hasAuthorship W2100648049A5008641700 @default.
• W2100648049 hasAuthorship W2100648049A5089627980 @default.
• W2100648049 hasBestOaLocation W21006480491 @default.
• W2100648049 hasConcept C104317684 @default.
• W2100648049 hasConcept C143065580 @default.
• W2100648049 hasConcept C153911025 @default.
• W2100648049 hasConcept C179185449 @default.
• W2100648049 hasConcept C185592680 @default.
• W2100648049 hasConcept C202751555 @default.
• W2100648049 hasConcept C2779454882 @default.
• W2100648049 hasConcept C501734568 @default.
• W2100648049 hasConcept C54355233 @default.
• W2100648049 hasConcept C547475151 @default.
• W2100648049 hasConcept C552990157 @default.
• W2100648049 hasConcept C55493867 @default.
• W2100648049 hasConcept C86803240 @default.
• W2100648049 hasConcept C9418097 @default.
• W2100648049 hasConceptScore W2100648049C104317684 @default.
• W2100648049 hasConceptScore W2100648049C143065580 @default.
• W2100648049 hasConceptScore W2100648049C153911025 @default.
• W2100648049 hasConceptScore W2100648049C179185449 @default.
• W2100648049 hasConceptScore W2100648049C185592680 @default.
• W2100648049 hasConceptScore W2100648049C202751555 @default.
• W2100648049 hasConceptScore W2100648049C2779454882 @default.
• W2100648049 hasConceptScore W2100648049C501734568 @default.
• W2100648049 hasConceptScore W2100648049C54355233 @default.
• W2100648049 hasConceptScore W2100648049C547475151 @default.
• W2100648049 hasConceptScore W2100648049C552990157 @default.
• W2100648049 hasConceptScore W2100648049C55493867 @default.
• W2100648049 hasConceptScore W2100648049C86803240 @default.
• W2100648049 hasConceptScore W2100648049C9418097 @default.
• W2100648049 hasIssue "4" @default.
• W2100648049 hasLocation W21006480491 @default.
• W2100648049 hasLocation W21006480492 @default.
• W2100648049 hasLocation W21006480493 @default.
• W2100648049 hasLocation W21006480494 @default.
• W2100648049 hasOpenAccess W2100648049 @default.
• W2100648049 hasPrimaryLocation W21006480491 @default.
• W2100648049 hasRelatedWork W1493361871 @default.
• W2100648049 hasRelatedWork W1548421761 @default.
• W2100648049 hasRelatedWork W1937520340 @default.
• W2100648049 hasRelatedWork W2002128513 @default.
• W2100648049 hasRelatedWork W2080910126 @default.
• W2100648049 hasRelatedWork W2358656917 @default.

• next