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• W2100654893 abstract "Two bacmid-derived Autographa californica Multiple-capsid Nucleopolyhedrovirus genotypes – that differ only in a short tag sequence for differential PCR recognition – were generated. By electron microscopy, these genotypes were found to have identical polyhedra morphology. Mixtures of quantified polyhedra were made and used to validate a SYBR Green I-based quantitative real-time PCR (qPCR) to determine genotype frequencies in mixed genotype populations. The PCR could accurately quantify genotype ratios over a range of 8 orders of magnitude. Only a small correction of the genotype ratio was necessary to obtain a valid result. Low levels of aspecific background (a fluorescent signal when the template corresponding with the primer set used is not present) were measured in these validation experiments and in a typical laboratory setup. A small fitness difference between the genotypes generated was observed in a median lethal dose bioassay. The bacmid-derived virus genotypes generated and the qPCR assays are valuable tools for studying the population biology of baculoviruses." @default.
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• W2100654893 date "2008-03-01" @default.
• W2100654893 modified "2023-10-15" @default.
• W2100654893 title "Development of a quantitative real-time PCR for determination of genotype frequencies for studies in baculovirus population biology" @default.
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• W2100654893 doi "https://doi.org/10.1016/j.jviromet.2007.10.022" @default.
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