FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2100693118> ?p ?o ?g. }

• W2100693118 endingPage "1072" @default.
• W2100693118 startingPage "1057" @default.
• W2100693118 abstract "Mounting environmental concerns associated with the use of petroleum-based chemical manufacturing practices has generated significant interest in the development of biological alternatives for the production of propionate. However, biological platforms for propionate production have been limited to strict anaerobes, such as Propionibacteria and select Clostridia. In this work, we demonstrated high-level heterologous production of propionate under microaerobic conditions in engineered Escherichia coli. Activation of the native Sleeping beauty mutase (Sbm) operon not only transformed E. coli to be propionogenic (i.e., propionate-producing) but also introduced an intracellular flux competition between the traditional C2-fermentative pathway and the novel C3-fermentative pathway. Dissimilation of the major carbon source of glycerol was identified to critically affect such flux competition and, therefore, propionate synthesis. As a result, the propionogenic E. coli was further engineered by inactivation or overexpression of various genes involved in the glycerol dissimilation pathways and their individual genetic effects on propionate production were investigated. Generally, knocking out genes involved in glycerol dissimilation (except glpA) can minimize levels of solventogenesis and shift more dissimilated carbon flux toward the C3-fermentative pathway. For optimal propionate production with high C3:C2-fermentative product ratios, glycerol dissimilation should be channeled through the respiratory pathway and, upon suppressed solventogenesis with minimal production of highly reduced alcohols, the alternative NADH-consuming route associated with propionate synthesis can be critical for more flexible redox balancing. With the implementation of various biochemical and genetic strategies, high propionate titers of more than 11 g/L with high yields up to 0.4 g-propionate/g-glycerol (accounting for ~50 % of dissimilated glycerol) were achieved, demonstrating the potential for industrial application. To our knowledge, this represents the most effective engineered microbial system for propionate production with titers and yields comparable to those achieved by anaerobic batch cultivation of various native propionate-producing strains of Propionibacteria." @default.
• W2100693118 created "2016-06-24" @default.
• W2100693118 creator A5001705178 @default.
• W2100693118 creator A5048660260 @default.
• W2100693118 creator A5056830639 @default.
• W2100693118 creator A5059914775 @default.
• W2100693118 creator A5079546995 @default.
• W2100693118 date "2015-07-01" @default.
• W2100693118 modified "2023-09-24" @default.
• W2100693118 title "Engineering <i>Escherichia coli</i> for high-level production of propionate" @default.
• W2100693118 cites W1482015224 @default.
• W2100693118 cites W1776978090 @default.
• W2100693118 cites W1781531090 @default.
• W2100693118 cites W1969529366 @default.
• W2100693118 cites W1985604744 @default.
• W2100693118 cites W1987005877 @default.
• W2100693118 cites W1989719822 @default.
• W2100693118 cites W1997970206 @default.
• W2100693118 cites W2002403955 @default.
• W2100693118 cites W2014361215 @default.
• W2100693118 cites W2023586880 @default.
• W2100693118 cites W2028054553 @default.
• W2100693118 cites W2031146914 @default.
• W2100693118 cites W2034748505 @default.
• W2100693118 cites W2038072588 @default.
• W2100693118 cites W2041567438 @default.
• W2100693118 cites W2042814467 @default.
• W2100693118 cites W2047529895 @default.
• W2100693118 cites W2051737550 @default.
• W2100693118 cites W2061838053 @default.
• W2100693118 cites W2064312004 @default.
• W2100693118 cites W2069568056 @default.
• W2100693118 cites W2074344367 @default.
• W2100693118 cites W2080021479 @default.
• W2100693118 cites W2089407963 @default.
• W2100693118 cites W2111967267 @default.
• W2100693118 cites W2112737296 @default.
• W2100693118 cites W2116137883 @default.
• W2100693118 cites W2127384727 @default.
• W2100693118 cites W2132417094 @default.
• W2100693118 cites W2132602091 @default.
• W2100693118 cites W2138530870 @default.
• W2100693118 cites W2142449740 @default.
• W2100693118 cites W2148033653 @default.
• W2100693118 cites W2148238060 @default.
• W2100693118 cites W2152875113 @default.
• W2100693118 cites W215452269 @default.
• W2100693118 cites W2155642589 @default.
• W2100693118 cites W2161580117 @default.
• W2100693118 cites W2163147351 @default.
• W2100693118 cites W2164870120 @default.
• W2100693118 cites W261856527 @default.
• W2100693118 cites W4244151339 @default.
• W2100693118 doi "https://doi.org/10.1007/s10295-015-1627-4" @default.
• W2100693118 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25948049" @default.
• W2100693118 hasPublicationYear "2015" @default.
• W2100693118 type Work @default.
• W2100693118 sameAs 2100693118 @default.
• W2100693118 citedByCount "29" @default.
• W2100693118 countsByYear W21006931182016 @default.
• W2100693118 countsByYear W21006931182017 @default.
• W2100693118 countsByYear W21006931182019 @default.
• W2100693118 countsByYear W21006931182020 @default.
• W2100693118 countsByYear W21006931182021 @default.
• W2100693118 countsByYear W21006931182022 @default.
• W2100693118 countsByYear W21006931182023 @default.
• W2100693118 crossrefType "journal-article" @default.
• W2100693118 hasAuthorship W2100693118A5001705178 @default.
• W2100693118 hasAuthorship W2100693118A5048660260 @default.
• W2100693118 hasAuthorship W2100693118A5056830639 @default.
• W2100693118 hasAuthorship W2100693118A5059914775 @default.
• W2100693118 hasAuthorship W2100693118A5079546995 @default.
• W2100693118 hasConcept C104317684 @default.
• W2100693118 hasConcept C138885662 @default.
• W2100693118 hasConcept C181199279 @default.
• W2100693118 hasConcept C185592680 @default.
• W2100693118 hasConcept C2780014933 @default.
• W2100693118 hasConcept C2780881558 @default.
• W2100693118 hasConcept C41895202 @default.
• W2100693118 hasConcept C547475151 @default.
• W2100693118 hasConcept C55493867 @default.
• W2100693118 hasConcept C6350086 @default.
• W2100693118 hasConcept C80316690 @default.
• W2100693118 hasConcept C86803240 @default.
• W2100693118 hasConcept C89423630 @default.
• W2100693118 hasConceptScore W2100693118C104317684 @default.
• W2100693118 hasConceptScore W2100693118C138885662 @default.
• W2100693118 hasConceptScore W2100693118C181199279 @default.
• W2100693118 hasConceptScore W2100693118C185592680 @default.
• W2100693118 hasConceptScore W2100693118C2780014933 @default.
• W2100693118 hasConceptScore W2100693118C2780881558 @default.
• W2100693118 hasConceptScore W2100693118C41895202 @default.
• W2100693118 hasConceptScore W2100693118C547475151 @default.
• W2100693118 hasConceptScore W2100693118C55493867 @default.
• W2100693118 hasConceptScore W2100693118C6350086 @default.
• W2100693118 hasConceptScore W2100693118C80316690 @default.
• W2100693118 hasConceptScore W2100693118C86803240 @default.
• W2100693118 hasConceptScore W2100693118C89423630 @default.

• next