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- W2100705886 abstract "A systematic optimization of the HPLC separation of a complex mixture containing urinary steroids (anabolics and corticoids), boldenone and bolasterone (synthetic anabolics) by micellar liquid chromatography has been carried out. The isocratic micellar mobile phases (from binary to quaternary) consisted of sodium dodecyl sulphate and organic modifiers such as acetonitrile, tetrahydrofuran, propanol, butanol or pentanol. The effect of the organic modifiers, surfactant concentration, temperature, ionic strength and flow-rate on the separation has been studied. A micellar mobile phase made of 5% propanol and 40 mM surfactant allowed the separation of 13 steroids in about 23 min. A bivariant optimization method for the micellar mobile phase surfactant-propanol corroborated the above results. The separations obtained show good perspectives for future developments." @default.
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- W2100705886 date "2003-09-01" @default.
- W2100705886 modified "2023-09-30" @default.
- W2100705886 title "Method development for corticosteroids and anabolic steroids by micellar liquid chromatography" @default.
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- W2100705886 doi "https://doi.org/10.1016/s1570-0232(03)00426-4" @default.
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