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- W2100706494 abstract "Purpose: To develop and validate an improved method by capillary zone electrophoresis with photodiode array detection for the fingerprint analysis of Ligusticum chuanxiong Hort. (Rhizoma Chuanxiong).Methods: The optimum high performance capillary electrophoresis (HPCE) conditions were 30 mM borax containing 5 %v/v acetonitrile at pH 9.6, direct injection of water extract at 0.5 psi for 5s, 25kV running voltage at positive power, 20 ℃ capillary temperature and 295 nm detection. Between injections, capillary was rinsed using 0.1M sodium hydroxide solution for 4 min, with HPCE-grade water for 4 min and re-equilibrated with running buffer for 2 min at 20 psi, moreover, the running buffer was replaced after three consecutive injections.Results: Baseline separation of most of the 17 detectable peaks in the water extract could be completed within 21 min.The relative standard deviations (RSD) of precision, reproducibility, and stability were < 2.79 % for retention time and 8.68 % for peak area of the main peaks.Conclusion: The developed method is simple, fast, reliable and environment-friendly for the fingerprint analysis of Rhizoma Chuanxiong water extract.Keywords: Capillary electrophoresis, Rhizoma chuanxiong, Water extract, Fingerprint analysis, Diode array" @default.
- W2100706494 created "2016-06-24" @default.
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- W2100706494 date "2014-09-11" @default.
- W2100706494 modified "2023-10-18" @default.
- W2100706494 title "Development and Validation of Improved Method for Fingerprint Analysis of <i>Rhizoma Chuanxiong</i> by Capillary Zone Electrophoresis with Ultraviolet-Diode Array Detection" @default.
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- W2100706494 doi "https://doi.org/10.4314/tjpr.v13i5.18" @default.
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