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- W2100782488 endingPage "2990" @default.
- W2100782488 startingPage "2981" @default.
- W2100782488 abstract "ABSTRACT The asymptomatic, chronic carrier state of Salmonella enterica serovar Typhi occurs in the bile-rich gallbladder and is frequently associated with the presence of cholesterol gallstones. We have previously demonstrated that salmonellae form biofilms on human gallstones and cholesterol-coated surfaces in vitro and that bile-induced biofilm formation on cholesterol gallstones promotes gallbladder colonization and maintenance of the carrier state. Random transposon mutants of S. enterica serovar Typhimurium were screened for impaired adherence to and biofilm formation on cholesterol-coated Eppendorf tubes but not on glass and plastic surfaces. We identified 49 mutants with this phenotype. The results indicate that genes involved in flagellum biosynthesis and structure primarily mediated attachment to cholesterol. Subsequent analysis suggested that the presence of the flagellar filament enhanced binding and biofilm formation in the presence of bile, while flagellar motility and expression of type 1 fimbriae were unimportant. Purified Salmonella flagellar proteins used in a modified enzyme-linked immunosorbent assay (ELISA) showed that FliC was the critical subunit mediating binding to cholesterol. These studies provide a better understanding of early events during biofilm development, specifically how salmonellae bind to cholesterol, and suggest a target for therapies that may alleviate biofilm formation on cholesterol gallstones and the chronic carrier state." @default.
- W2100782488 created "2016-06-24" @default.
- W2100782488 creator A5007923924 @default.
- W2100782488 creator A5012664199 @default.
- W2100782488 creator A5082003915 @default.
- W2100782488 date "2010-06-15" @default.
- W2100782488 modified "2023-10-03" @default.
- W2100782488 title "Flagellated but Not Hyperfimbriated <i>Salmonella enterica</i> Serovar Typhimurium Attaches to and Forms Biofilms on Cholesterol-Coated Surfaces" @default.
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