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- W2100801924 abstract "In this study, a sensitive, specific assay for the determination of TNP-470 and its two major metabolites M-IV (also know as AGM-1883) and M-II in human plasma is reported. The assay involves liquid-liquid extraction of acidified plasma followed by reversed-phase high-performance liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry. A liquid-liquid extraction using an organic solvent mixture (methyl-tert-butyl-ether-hexane, 1:1, v/v) is used in place of solid-phase extraction because it provides consistent recoveries for all analytes, including the internal standard. Retention times for the analytes and internal standard are less than 7 min. Within- and between-day precision is < or = 6.5% and < or = 13.3% relative standard deviation, respectively, for the three analytes. The lower limits of quantitation are 0.25, 0.5, and 1.0 ng/mL for TNP-470, M-IV, and M-II, respectively." @default.
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- W2100801924 date "2002-04-01" @default.
- W2100801924 modified "2023-09-28" @default.
- W2100801924 title "Assay of TNP-470 and Its Two Major Metabolites in Human Plasma by High-Performance Liquid Chromatography-Mass Spectrometry" @default.
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- W2100801924 doi "https://doi.org/10.1093/chromsci/40.4.214" @default.
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