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- W2100847580 abstract "A simple competitive strategy was designed for the sensitive detection of sequence-specific DNA by combining endonuclease-assisted target recycling and electrochemical stripping analysis of silver nanoparticles (AgNPs). The AgNP-tagged carbon nanospheres were synthesized by means of in situ reduction of Ag+ adsorbed onto a negatively charged polyelectrolyte layer and functionalized with streptavidin for binding biotin-labeled DNA strands. The labeled strand was captured on the DNA sensor surface by competitive hybridization of biotinated primer 1 and its cleaved product. The cleaved product could be amplified in homogeneous solution by endonuclease-assisted target recycling with a Y-shaped junction DNA structure, thus leading to the correlation of the stripping signal to the target concentration. The functionalized nanosphere was characterized with X-ray photoelectron spectroscopy, scanning electron microscopy, and transmission electron microscopy. The proposed method showed a linear range from 0.1 to 1000 fM with a limit of detection of 0.066 fM (3σ) and good selectivity for base discrimination. The designed strategy provided a sensitive tool for DNA analysis and could be widely applied in bioanalysis and biomedicine." @default.
- W2100847580 created "2016-06-24" @default.
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- W2100847580 date "2012-09-17" @default.
- W2100847580 modified "2023-10-16" @default.
- W2100847580 title "A Competitive Strategy Coupled with Endonuclease-Assisted Target Recycling for DNA Detection Using Silver-Nanoparticle-Tagged Carbon Nanospheres as Labels" @default.
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- W2100847580 doi "https://doi.org/10.1002/chem.201201307" @default.
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