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• W2100973952 abstract "Alcohol dehydrogenase beta subunit (ADH2) Arg47His and aldehyde dehydrogenase 2 (ALDH2) Glu487Lys were genotyped by a duplex polymerase chain reaction (PCR) with confronting two-pair primers (PCR-CTPP), which allows DNA amplification with one-tube PCR including eight primers, and subsequent electrophoresis.Several PCR conditions were tested to establish the optimal conditions for distinguishing the allele-specific bands for the two polymorphisms. Under the optimal PCR conditions, 454 Japanese health check-up examinees were genotyped.The allele-specific bands were successfully amplified under the optimal conditions of the duplex PCR-CTPP. The genotype distributions were within the Hardy-Weinberg equilibrium. The bands produced by the duplex PCR-CTPP genotyping were clearer than those produced by PCR-CTPP, conducted solely for ADH2.ADH2 Arg47His and ALDH2 Glu487Lys were successfully genotyped by this newly developed duplex PCR-CTPP, an inexpensive and time-saving genotyping tool, which will be useful in epidemiological studies on alcoholism, as well as risk estimation of alcohol-related diseases." @default.
• W2100973952 created "2016-06-24" @default.
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• W2100973952 date "2003-08-12" @default.
• W2100973952 modified "2023-09-28" @default.
• W2100973952 title "DUPLEX POLYMERASE CHAIN REACTION WITH CONFRONTING TWO-PAIR PRIMERS (PCR-CTPP) FOR GENOTYPING ALCOHOL DEHYDROGENASE SUBUNIT (ADH2) AND ALDEHYDE DEHYDROGENASE 2 (ALDH2)" @default.
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• W2100973952 doi "https://doi.org/10.1093/alcalc/agg096" @default.
• W2100973952 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12915514" @default.
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