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- W2101090830 abstract "University of Medical SciencesŚw. Marii Magdaleny 1461-861 Poznan, Poland*corresponding author: tel.: +4861 6659550, fax: +4861 6659551, e-mail: agnieszka.gryszczynska@iwnirz.plSummaryThe purpose of presented study was the comparison of flavan-3-ol content in the roots of Rhodiola kirilowii and Rhodiola rosea with use of UPLC-MS/MS method. Two kinds of ex-tract were prepared: aqueous extract and 50% v/v ethanol extract. The worked out UPLC MS/MS method allowed to determine the content of five flavan-3-ols: (+)-catechin, (-)-epi-catechin, (-)-epigallocatechin, (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG). The obtained results shown that the content of measured catechins was higher in R kirilowii roots than in R rosea. Both Rhodiola roots contain EGCG as a main proanthocya-nidin compound – the content in R kirilowii roots is about 0.14%. Our results indicate that application of ultra performance liquid chromatograph connected to a tandem mass spectrometer (UPLC MS/MS method) allows to determine the proanthocyanidins content in tested samples with satisfactory precision and can be used in the Rhodiola sp. phyto-chemical investigations.Key words: Rhodiola kirilowii, Rhodiola rosea, flavan-3-ol content, UPLC-MS/MS method, (-)-epi-gallocatechin gallate (EGCG)" @default.
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- W2101090830 date "2012-01-01" @default.
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- W2101090830 title "Comparison of proanthocyanidins content in Rhodiola kirilowii and Rhodiola rosea roots – application of UPLC-MS/MS method" @default.
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