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• W2101255471 abstract "Sixty-eigth out of 530 different S. aureus field strains isolated from subclinical cases of bovine mastitis from Germany (n = 26), Indonesia (n = 16), Mexico (n = 16) and Brazil (n = 10), respectively, were selected to be studied in the present work. The strains were tested phenotypically as well as genotypically for the presence of penicillin G- and oxacillin-resistance. For the primary phenotypical species identification of the 530 S. aureus strains, plasmacoagulase-test and Api 32 Staph system was applied. This was confirmed by molecular detection of the S. aureus specific genes encoding 23 S rRNA, thermostable nuclease ( nuc ), clumping factor ( clf A), coagulase ( coa ) and protein A region Xr ( spa ). The selection of the 68 strains was carried out by the random selection of one strain per herd; additionally, only strains with different macrorestriction profiles were included here. Genotypic resistance to semisynthetic penicillins (methicillin/oxacillin) and penicillin G was studied through the identification of mec A- and bla Z-genes, respectively. The mec A gene was detected in only one S. aureus isolate from Brazil, which was not phenotypically resistant against methcillin, as shown by the use of standard disc diffusion method, BBL-Chromoagar and MIC-determination by Vitek II. In contrast penicillin-resistance of strains based on the presence of the bla Z-gene could be ob­served in 50 (73.5%) of the investigated strains. However, only 40 (58.8%) of these 50 blaZ-positive strains were phenotypica­lly penicillin-resistant. According to the presented data, resistance to semisynthetic penicillins in S. aureus field strains seems to be not a major problem in dairy herds of the investigated countries despite the long-term use of these antibiotics in the field." @default.
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• W2101255471 date "2011-09-27" @default.
• W2101255471 modified "2023-09-23" @default.
• W2101255471 title "Resistencia a penicilina G y oxacilina, de cepas de Staphylococcus aureus aisladas de mastitis bovina subclínica" @default.
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