Matches in SemOpenAlex for { <https://semopenalex.org/work/W2101410077> ?p ?o ?g. }
- W2101410077 endingPage "186" @default.
- W2101410077 startingPage "173" @default.
- W2101410077 abstract "Abstract Fluorescence, CD, and activity measurements were used to characterize the different conformational states of horseradish peroxidase A1 induced by thermal unfolding. Picosecond time‐resolved fluorescence studies showed a three‐exponential decay dominated by a picosecond lifetime component resulting from energy transfer from tryptophan to heme. Upon thermal unfolding a decrease in the preexponential factor of the picosecond lifetime and an increase in the quantum yield were observed approaching the characteristics observed for apoHRPA1. The fraction of heme‐quenched fluorophore decreased to 0.4 after unfolding as shown by acrylamide quenching. A new unfolding pathway for HRPA1 was proposed and the effect of the low molecular weight solutes trehalose, sorbitol, and melezitose on this pathway was analyzed. Native HRPA1 unfolds with an intermediate between the native and the unfolded conformation. The unfolded conformation can refold to the native state or to a native‐like conformation with no calcium ions upon cooling or can give an irreversible denatured state. The refolded conformation with no calcium ions was clearly identified in a second thermal scan in the presence of EDTA and shows secondary and tertiary structures, heme reincorporation in the cavity, and at least 59% of activity. Melezitose stabilized the refolded Ca 2+ ‐depleted protein and induced a more complex mechanism for heme disruption. The effect of sorbitol and trehalose were mainly characterized by an increase in the temperature of unfolding. © 2004 Wiley Periodicals, Inc. Biopolymers, 2004" @default.
- W2101410077 created "2016-06-24" @default.
- W2101410077 creator A5030800506 @default.
- W2101410077 creator A5046383668 @default.
- W2101410077 creator A5078249721 @default.
- W2101410077 creator A5080615404 @default.
- W2101410077 creator A5081316321 @default.
- W2101410077 creator A5087887712 @default.
- W2101410077 date "2004-07-21" @default.
- W2101410077 modified "2023-10-14" @default.
- W2101410077 title "Conformational states of HRPA1 induced by thermal unfolding: Effect of low molecular weight solutes" @default.
- W2101410077 cites W1498222524 @default.
- W2101410077 cites W1536654439 @default.
- W2101410077 cites W1550362445 @default.
- W2101410077 cites W1581948411 @default.
- W2101410077 cites W1582395028 @default.
- W2101410077 cites W1601666612 @default.
- W2101410077 cites W1606676886 @default.
- W2101410077 cites W1656759821 @default.
- W2101410077 cites W1967817108 @default.
- W2101410077 cites W1972161729 @default.
- W2101410077 cites W1972595839 @default.
- W2101410077 cites W1973989038 @default.
- W2101410077 cites W1987208202 @default.
- W2101410077 cites W1988063569 @default.
- W2101410077 cites W2006824200 @default.
- W2101410077 cites W2006982266 @default.
- W2101410077 cites W2007157911 @default.
- W2101410077 cites W2015550998 @default.
- W2101410077 cites W2016815768 @default.
- W2101410077 cites W2025969336 @default.
- W2101410077 cites W2028491723 @default.
- W2101410077 cites W2029926648 @default.
- W2101410077 cites W2031694098 @default.
- W2101410077 cites W2040883347 @default.
- W2101410077 cites W2041879090 @default.
- W2101410077 cites W2055676171 @default.
- W2101410077 cites W2055678974 @default.
- W2101410077 cites W2055955041 @default.
- W2101410077 cites W2062261789 @default.
- W2101410077 cites W2068010309 @default.
- W2101410077 cites W2078890539 @default.
- W2101410077 cites W2085501958 @default.
- W2101410077 cites W2087769394 @default.
- W2101410077 cites W2088218075 @default.
- W2101410077 cites W2089037490 @default.
- W2101410077 cites W2089357027 @default.
- W2101410077 cites W2098807743 @default.
- W2101410077 cites W2129995310 @default.
- W2101410077 cites W2158585378 @default.
- W2101410077 cites W2162612379 @default.
- W2101410077 cites W2165898505 @default.
- W2101410077 cites W2311358531 @default.
- W2101410077 cites W2410824620 @default.
- W2101410077 cites W4376595447 @default.
- W2101410077 cites W3142063049 @default.
- W2101410077 doi "https://doi.org/10.1002/bip.20100" @default.
- W2101410077 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15356871" @default.
- W2101410077 hasPublicationYear "2004" @default.
- W2101410077 type Work @default.
- W2101410077 sameAs 2101410077 @default.
- W2101410077 citedByCount "9" @default.
- W2101410077 countsByYear W21014100772013 @default.
- W2101410077 crossrefType "journal-article" @default.
- W2101410077 hasAuthorship W2101410077A5030800506 @default.
- W2101410077 hasAuthorship W2101410077A5046383668 @default.
- W2101410077 hasAuthorship W2101410077A5078249721 @default.
- W2101410077 hasAuthorship W2101410077A5080615404 @default.
- W2101410077 hasAuthorship W2101410077A5081316321 @default.
- W2101410077 hasAuthorship W2101410077A5087887712 @default.
- W2101410077 hasConcept C121332964 @default.
- W2101410077 hasConcept C121745418 @default.
- W2101410077 hasConcept C12554922 @default.
- W2101410077 hasConcept C181199279 @default.
- W2101410077 hasConcept C185592680 @default.
- W2101410077 hasConcept C19472624 @default.
- W2101410077 hasConcept C2776217839 @default.
- W2101410077 hasConcept C2778452849 @default.
- W2101410077 hasConcept C55493867 @default.
- W2101410077 hasConcept C62520636 @default.
- W2101410077 hasConcept C75473681 @default.
- W2101410077 hasConcept C8010536 @default.
- W2101410077 hasConcept C86803240 @default.
- W2101410077 hasConcept C91881484 @default.
- W2101410077 hasConcept C96305047 @default.
- W2101410077 hasConceptScore W2101410077C121332964 @default.
- W2101410077 hasConceptScore W2101410077C121745418 @default.
- W2101410077 hasConceptScore W2101410077C12554922 @default.
- W2101410077 hasConceptScore W2101410077C181199279 @default.
- W2101410077 hasConceptScore W2101410077C185592680 @default.
- W2101410077 hasConceptScore W2101410077C19472624 @default.
- W2101410077 hasConceptScore W2101410077C2776217839 @default.
- W2101410077 hasConceptScore W2101410077C2778452849 @default.
- W2101410077 hasConceptScore W2101410077C55493867 @default.
- W2101410077 hasConceptScore W2101410077C62520636 @default.
- W2101410077 hasConceptScore W2101410077C75473681 @default.
- W2101410077 hasConceptScore W2101410077C8010536 @default.
- W2101410077 hasConceptScore W2101410077C86803240 @default.