FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2101428477> ?p ?o ?g. }

• W2101428477 endingPage "414" @default.
• W2101428477 startingPage "403" @default.
• W2101428477 abstract "Abstract To understand how the photoreceptor protein rhodopsin performs in its role as a receptor, its structure needs to be determined at the atomic level. Upon receiving a photon of light, rhodopsin undergoes a change in conformation that allows it to bind and activate the C-protein, transducin. An important future goal should be to determine the structure of both the inactive and the photoactivated state of rhodopsin, R*. This should provide the groundwork necessary for experiments on how rhodopsin achieves its signaling state R*, and how R* functions to activate transducin. To do this, the crystal structure of both rhodopsin and R* must be determined. Few membrane proteins have been successfully crystallized, so this is not a trivial undertaking. Two- or three dimensional crystals of rhodopsin must be prepared that are well ordered, to produce a high-resolution structure. Rhodopsin must be purified to homogeneity and the appropriate detergent(s) selected for crystallization experiments. Long-term thermal stability of the rhodopsin-detergent complex must be achieved in the presence of a precipitant. Two-dimensional crystals may offer advantages in investigating the structure of R*, but the structure obtained may be limited in resolution. It is necessary to work with rhodopsin in the dark, unless suitable light-stable retinal derivatives are developed. Protein engineering of rhodopsin offers attractive opportunities to improve its ability to crystallize, but is presently hindered by the absence of a high-yielding expression system. Knowledge of the structure of rhodopsin will have general importance. Because rhodopsin is a member of the family of C-protein-coupled receptors, knowledge of the structure and the mechanism of action of rhodopsin suggests by analogy how other members of the receptor family may function." @default.
• W2101428477 created "2016-06-24" @default.
• W2101428477 creator A5075791730 @default.
• W2101428477 date "1995-09-01" @default.
• W2101428477 modified "2023-09-27" @default.
• W2101428477 title "Future directions for rhodopsin structure and function studies" @default.
• W2101428477 cites W10086421 @default.
• W2101428477 cites W101220866 @default.
• W2101428477 cites W1034474538 @default.
• W2101428477 cites W1044930 @default.
• W2101428477 cites W1168957640 @default.
• W2101428477 cites W1245933497 @default.
• W2101428477 cites W1274782733 @default.
• W2101428477 cites W1277716736 @default.
• W2101428477 cites W13782808 @default.
• W2101428477 cites W138382208 @default.
• W2101428477 cites W1445935732 @default.
• W2101428477 cites W1451251438 @default.
• W2101428477 cites W1480773806 @default.
• W2101428477 cites W1483634733 @default.
• W2101428477 cites W1485501181 @default.
• W2101428477 cites W1488151070 @default.
• W2101428477 cites W1488367823 @default.
• W2101428477 cites W1492780137 @default.
• W2101428477 cites W1494856533 @default.
• W2101428477 cites W1494933378 @default.
• W2101428477 cites W1501713341 @default.
• W2101428477 cites W1503305141 @default.
• W2101428477 cites W1504397462 @default.
• W2101428477 cites W1506213416 @default.
• W2101428477 cites W1506764244 @default.
• W2101428477 cites W1508171779 @default.
• W2101428477 cites W1508376267 @default.
• W2101428477 cites W1508886543 @default.
• W2101428477 cites W1510130280 @default.
• W2101428477 cites W1514044463 @default.
• W2101428477 cites W1514240524 @default.
• W2101428477 cites W1515512367 @default.
• W2101428477 cites W1515625266 @default.
• W2101428477 cites W1517947547 @default.
• W2101428477 cites W1518396254 @default.
• W2101428477 cites W1522870006 @default.
• W2101428477 cites W1525024462 @default.
• W2101428477 cites W1525041374 @default.
• W2101428477 cites W1525241413 @default.
• W2101428477 cites W1525266792 @default.
• W2101428477 cites W1528353095 @default.
• W2101428477 cites W1529483913 @default.
• W2101428477 cites W1530456903 @default.
• W2101428477 cites W1533887373 @default.
• W2101428477 cites W1533937831 @default.
• W2101428477 cites W1534114330 @default.
• W2101428477 cites W1535774104 @default.
• W2101428477 cites W1536183747 @default.
• W2101428477 cites W1538055012 @default.
• W2101428477 cites W1549334326 @default.
• W2101428477 cites W1549583981 @default.
• W2101428477 cites W1551037687 @default.
• W2101428477 cites W1551152196 @default.
• W2101428477 cites W1554226168 @default.
• W2101428477 cites W1554470867 @default.
• W2101428477 cites W1556609530 @default.
• W2101428477 cites W1557961309 @default.
• W2101428477 cites W1558585768 @default.
• W2101428477 cites W1558733231 @default.
• W2101428477 cites W1558921401 @default.
• W2101428477 cites W1562615854 @default.
• W2101428477 cites W1563310899 @default.
• W2101428477 cites W1564406528 @default.
• W2101428477 cites W1565985941 @default.
• W2101428477 cites W1566050966 @default.
• W2101428477 cites W1566199310 @default.
• W2101428477 cites W1566233194 @default.
• W2101428477 cites W1567325248 @default.
• W2101428477 cites W1567653349 @default.
• W2101428477 cites W1570035827 @default.
• W2101428477 cites W1570444949 @default.
• W2101428477 cites W1570762629 @default.
• W2101428477 cites W1571044863 @default.
• W2101428477 cites W1571104445 @default.
• W2101428477 cites W1571668808 @default.
• W2101428477 cites W1575279402 @default.
• W2101428477 cites W1575693343 @default.
• W2101428477 cites W1575883584 @default.
• W2101428477 cites W1576313910 @default.
• W2101428477 cites W1576457008 @default.
• W2101428477 cites W1577989916 @default.
• W2101428477 cites W1579091371 @default.
• W2101428477 cites W1581444291 @default.
• W2101428477 cites W1583253515 @default.
• W2101428477 cites W1584284659 @default.
• W2101428477 cites W1584684122 @default.
• W2101428477 cites W1587185311 @default.
• W2101428477 cites W1589816259 @default.
• W2101428477 cites W1590595627 @default.
• W2101428477 cites W1590951841 @default.
• W2101428477 cites W1591746786 @default.
• W2101428477 cites W1592692300 @default.

• next