Matches in SemOpenAlex for { <https://semopenalex.org/work/W2101567225> ?p ?o ?g. }
- W2101567225 endingPage "e13761" @default.
- W2101567225 startingPage "e13761" @default.
- W2101567225 abstract "The light-gated cation channel Channelrhodopsin-2 (ChR2) is a powerful and versatile tool for controlling neuronal activity. Currently available versions of ChR2 either distribute uniformly throughout the plasma membrane or are localised specifically to somatodendritic or synaptic domains. Localising ChR2 instead to the axon initial segment (AIS) could prove an extremely useful addition to the optogenetic repertoire, targeting the channel directly to the site of action potential initiation, and limiting depolarisation and associated calcium entry elsewhere in the neuron. Here, we describe a ChR2 construct that we localised specifically to the AIS by adding the ankyrinG-binding loop of voltage-gated sodium channels (Na(v)II-III) to its intracellular terminus. Expression of ChR2-YFP-Na(v)II-III did not significantly affect the passive or active electrical properties of cultured rat hippocampal neurons. However, the tiny ChR2 currents and small membrane depolarisations resulting from AIS targeting meant that optogenetic control of action potential firing with ChR2-YFP-Na(v)II-III was unsuccessful in baseline conditions. We did succeed in stimulating action potentials with light in some ChR2-YFP-Na(v)II-III-expressing neurons, but only when blocking KCNQ voltage-gated potassium channels. We discuss possible alternative approaches to obtaining precise control of neuronal spiking with AIS-targeted optogenetic constructs and propose potential uses for our ChR2-YFP-Na(v)II-III probe where subthreshold modulation of action potential initiation is desirable." @default.
- W2101567225 created "2016-06-24" @default.
- W2101567225 creator A5047455423 @default.
- W2101567225 creator A5065791692 @default.
- W2101567225 date "2010-10-29" @default.
- W2101567225 modified "2023-09-30" @default.
- W2101567225 title "Channelrhodopsin-2 Localised to the Axon Initial Segment" @default.
- W2101567225 cites W1599277155 @default.
- W2101567225 cites W1606137504 @default.
- W2101567225 cites W1652102492 @default.
- W2101567225 cites W1863753209 @default.
- W2101567225 cites W1963937305 @default.
- W2101567225 cites W1969483526 @default.
- W2101567225 cites W1971618024 @default.
- W2101567225 cites W1973967768 @default.
- W2101567225 cites W1980737345 @default.
- W2101567225 cites W1986142798 @default.
- W2101567225 cites W1988862252 @default.
- W2101567225 cites W1989092549 @default.
- W2101567225 cites W1989809732 @default.
- W2101567225 cites W1991444801 @default.
- W2101567225 cites W1992406434 @default.
- W2101567225 cites W1992770435 @default.
- W2101567225 cites W1995275820 @default.
- W2101567225 cites W1995454338 @default.
- W2101567225 cites W1997809327 @default.
- W2101567225 cites W1998765922 @default.
- W2101567225 cites W1999311112 @default.
- W2101567225 cites W2000681405 @default.
- W2101567225 cites W2002812952 @default.
- W2101567225 cites W2006393959 @default.
- W2101567225 cites W2008758574 @default.
- W2101567225 cites W2010975466 @default.
- W2101567225 cites W2011973432 @default.
- W2101567225 cites W2012041126 @default.
- W2101567225 cites W2012147706 @default.
- W2101567225 cites W2015026123 @default.
- W2101567225 cites W2016706151 @default.
- W2101567225 cites W2021951036 @default.
- W2101567225 cites W2023969516 @default.
- W2101567225 cites W2024281115 @default.
- W2101567225 cites W2029428999 @default.
- W2101567225 cites W2034064942 @default.
- W2101567225 cites W2034208991 @default.
- W2101567225 cites W2034516685 @default.
- W2101567225 cites W2036048932 @default.
- W2101567225 cites W2037018268 @default.
- W2101567225 cites W2037594700 @default.
- W2101567225 cites W2038294038 @default.
- W2101567225 cites W2039347400 @default.
- W2101567225 cites W2039623701 @default.
- W2101567225 cites W2043204092 @default.
- W2101567225 cites W2043358402 @default.
- W2101567225 cites W2044098590 @default.
- W2101567225 cites W2046053065 @default.
- W2101567225 cites W2046501957 @default.
- W2101567225 cites W2048635483 @default.
- W2101567225 cites W2049779229 @default.
- W2101567225 cites W2052191318 @default.
- W2101567225 cites W2053568595 @default.
- W2101567225 cites W2055127006 @default.
- W2101567225 cites W2057222381 @default.
- W2101567225 cites W2057253193 @default.
- W2101567225 cites W2059485600 @default.
- W2101567225 cites W2065416066 @default.
- W2101567225 cites W2067330717 @default.
- W2101567225 cites W2068467384 @default.
- W2101567225 cites W2070691915 @default.
- W2101567225 cites W2071676133 @default.
- W2101567225 cites W2079282983 @default.
- W2101567225 cites W2079747449 @default.
- W2101567225 cites W2082160346 @default.
- W2101567225 cites W2083932518 @default.
- W2101567225 cites W2084811348 @default.
- W2101567225 cites W2085393352 @default.
- W2101567225 cites W2086837225 @default.
- W2101567225 cites W2087805590 @default.
- W2101567225 cites W2088595917 @default.
- W2101567225 cites W2088944569 @default.
- W2101567225 cites W2089845241 @default.
- W2101567225 cites W2091056275 @default.
- W2101567225 cites W2092166405 @default.
- W2101567225 cites W2096424464 @default.
- W2101567225 cites W2104755463 @default.
- W2101567225 cites W2106580108 @default.
- W2101567225 cites W2107738760 @default.
- W2101567225 cites W2108234653 @default.
- W2101567225 cites W2108846059 @default.
- W2101567225 cites W2110665798 @default.
- W2101567225 cites W2112938540 @default.
- W2101567225 cites W2115859705 @default.
- W2101567225 cites W2118950242 @default.
- W2101567225 cites W2119070939 @default.
- W2101567225 cites W2125095601 @default.
- W2101567225 cites W2125760448 @default.
- W2101567225 cites W2125773804 @default.
- W2101567225 cites W2127183925 @default.
- W2101567225 cites W2127780691 @default.