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• W2101680510 abstract "The recent development of yeast artificial chromosome (YAC) vectors has provided a system for cloning fragments that are over ten times larger than those that can be cloned in more established systems. We have developed a method for the rapid isolation of terminal sequences from YAC clones. The YAC clone is digested with a range of restriction enzymes, a common linker is ligated to the DNA fragments and terminal sequences are amplified using a vector specific primer and a linker specific primer. Sequence data derived from these terminal specific products can be used to design primers for a further round of screening to isolate overlapping clones. The method also provides a convenient method of generating Sequence Tagged Sites for the mapping of complex genomes." @default.
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• W2101680510 date "1990-01-01" @default.
• W2101680510 modified "2023-10-13" @default.
• W2101680510 title "<i>Cis</i>-acting elements required for RNA polymerase II and III transcription in the human U2 and U6 snRNA promoters" @default.
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• W2101680510 doi "https://doi.org/10.1093/nar/18.10.2891" @default.
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