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• W2101794645 abstract "Human TAF II 55 (hTAF II 55), a component of the general transcription factor TFIID, is the only general transcription factor encoded by an intronless gene identified thus far. Analysis of the TATA‐less hTAF II 55 promoter‐proximal sequence reveals putative binding sites for STAT‐1, MEF2, E2F, Sp1, AP2, AREB6 and E47. Using chromatin immunoprecipitation, DNase I footprinting and electrophoretic mobility shift assays, we demonstrate that Sp1 and AP2 can bind simultaneously to juxtaposed Sp1‐ and AP2‐binding sites in the hTAF II 55 promoter‐proximal region and functionally modulate hTAF II 55 promoter activity, as evidenced by reporter gene assays performed in transiently transfected human C‐33A and insect SL2 cell lines. Interestingly, removal of all the promoter‐proximal Sp1‐binding sites does not impair the function of the hTAF II 55 core promoter. Moreover, a 52‐bp DNA fragment containing only the hTAF II 55 initiator (Inr) and downstream promoter element (DPE) is able to support Gal4–VP16‐mediated activation in vivo and in vitro . Our data suggest that Sp1, although it plays an enhancing role in hTAF II 55 gene expression, is not essential for hTAF II 55 core promoter activity. Interestingly, mutations introduced at the Inr and DPE differentially affect the selection of transcription start sites, suggesting that these two core promoter elements play a non‐redundant role in the function of TATA‐less promoters." @default.
• W2101794645 created "2016-06-24" @default.
• W2101794645 creator A5002452221 @default.
• W2101794645 creator A5087326265 @default.
• W2101794645 date "2002-10-01" @default.
• W2101794645 modified "2023-10-04" @default.
• W2101794645 title "Sp1 and AP2 regulate but do not constitute TATA-less human TAFII55 core promoter activity" @default.
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• W2101794645 doi "https://doi.org/10.1093/nar/gkf537" @default.
• W2101794645 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/140537" @default.
• W2101794645 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12364593" @default.
• W2101794645 hasPublicationYear "2002" @default.
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